Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteria

Abstract Endophytic bacteria (EB) isolated from healthy cucumber plant tissues (e.g., root, stem, leaves) were evaluated as possible biological control of Pseudomonas syringae pv. lachrymans, the causative agent of angular leaf spot disease in cucumber. In this study, 24 endophytic bacteria were sel...

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Main Authors: Mustafa Akbaba, Hatice Ozaktan
Format: Article
Language:English
Published: SpringerOpen 2018-02-01
Series:Egyptian Journal of Biological Pest Control
Subjects:
Online Access:http://link.springer.com/article/10.1186/s41938-017-0020-1
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author Mustafa Akbaba
Hatice Ozaktan
author_facet Mustafa Akbaba
Hatice Ozaktan
author_sort Mustafa Akbaba
collection DOAJ
description Abstract Endophytic bacteria (EB) isolated from healthy cucumber plant tissues (e.g., root, stem, leaves) were evaluated as possible biological control of Pseudomonas syringae pv. lachrymans, the causative agent of angular leaf spot disease in cucumber. In this study, 24 endophytic bacteria were selected for tests based on biocontrol traits such as indole-3-acetic acid and siderophore production, solubilization of phosphate, and inhibition growth of P. syringae pv. lachrymans in vitro. Some of the selected endophytes successfully inhibited the pathogen were tested in pots. In the pot experiment, two isolates (CB361-80) and (CC372-83) were selected as the most promising biocontrol agents tested against the pathogen. According to the identification carried out by using 16S rRNA primers and sequence analysis, the two selected endophyte isolates (CB361-80) and (CC372-83) showed 99% similarity to Ochrobactrum pseudintermedium and Pantoea agglomerans, respectively. Two EB isolates colonized seeds at the rate of 3 × 106–7.1 × 107 CFU/g 1 day after seed bacterization. The population of the two EB isolates in cucumber tissues were found to be about 2 × 104–7.5 × 104 CFU/g in roots and 3.8 × 104–2.2 × 105 CFU/g in shoots 45 days after seed bacterization. The populations of the two isolates in the root and shoot tissues did not decrease following the inoculation of the pathogenic bacterium. The endophytic strains CB361-80 (Ochrobactrum sp.) and CC372-83 (Pantoea sp.) successfully colonized the cucumber tissues.
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spelling doaj.art-064599ddb91640c8a48d6c47333b05332022-12-21T18:37:36ZengSpringerOpenEgyptian Journal of Biological Pest Control2536-93422018-02-0128111010.1186/s41938-017-0020-1Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteriaMustafa Akbaba0Hatice Ozaktan1Department of Plant Protection, Faculty of Agriculture, University of EgeDepartment of Plant Protection, Faculty of Agriculture, University of EgeAbstract Endophytic bacteria (EB) isolated from healthy cucumber plant tissues (e.g., root, stem, leaves) were evaluated as possible biological control of Pseudomonas syringae pv. lachrymans, the causative agent of angular leaf spot disease in cucumber. In this study, 24 endophytic bacteria were selected for tests based on biocontrol traits such as indole-3-acetic acid and siderophore production, solubilization of phosphate, and inhibition growth of P. syringae pv. lachrymans in vitro. Some of the selected endophytes successfully inhibited the pathogen were tested in pots. In the pot experiment, two isolates (CB361-80) and (CC372-83) were selected as the most promising biocontrol agents tested against the pathogen. According to the identification carried out by using 16S rRNA primers and sequence analysis, the two selected endophyte isolates (CB361-80) and (CC372-83) showed 99% similarity to Ochrobactrum pseudintermedium and Pantoea agglomerans, respectively. Two EB isolates colonized seeds at the rate of 3 × 106–7.1 × 107 CFU/g 1 day after seed bacterization. The population of the two EB isolates in cucumber tissues were found to be about 2 × 104–7.5 × 104 CFU/g in roots and 3.8 × 104–2.2 × 105 CFU/g in shoots 45 days after seed bacterization. The populations of the two isolates in the root and shoot tissues did not decrease following the inoculation of the pathogenic bacterium. The endophytic strains CB361-80 (Ochrobactrum sp.) and CC372-83 (Pantoea sp.) successfully colonized the cucumber tissues.http://link.springer.com/article/10.1186/s41938-017-0020-1Biological controlCucumberEndophytic colonizationPseudomonas syringae pv. lachrymansOchrobactrum pseudintermediumPantoea agglomerans
spellingShingle Mustafa Akbaba
Hatice Ozaktan
Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteria
Egyptian Journal of Biological Pest Control
Biological control
Cucumber
Endophytic colonization
Pseudomonas syringae pv. lachrymans
Ochrobactrum pseudintermedium
Pantoea agglomerans
title Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteria
title_full Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteria
title_fullStr Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteria
title_full_unstemmed Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteria
title_short Biocontrol of angular leaf spot disease and colonization of cucumber (Cucumis sativus L.) by endophytic bacteria
title_sort biocontrol of angular leaf spot disease and colonization of cucumber cucumis sativus l by endophytic bacteria
topic Biological control
Cucumber
Endophytic colonization
Pseudomonas syringae pv. lachrymans
Ochrobactrum pseudintermedium
Pantoea agglomerans
url http://link.springer.com/article/10.1186/s41938-017-0020-1
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