The Antimicrobial Peptide LJ-hep2 from <i>Lateolabrax japonicus</i> Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo
Hepcidin is widely present in many kinds of fish and is an important innate immune factor. A variety of HAMP2-type hepcidins have strong antimicrobial activity and immunomodulatory functions and are expected to be developed as substitutes for antibiotics. In this study, the antimicrobial activity of...
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MDPI AG
2022-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/10/651 |
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| author | Ruihao Gong Zhe An Weibin Zhang Fangyi Chen Ke-Jian Wang |
| author_facet | Ruihao Gong Zhe An Weibin Zhang Fangyi Chen Ke-Jian Wang |
| author_sort | Ruihao Gong |
| collection | DOAJ |
| description | Hepcidin is widely present in many kinds of fish and is an important innate immune factor. A variety of HAMP2-type hepcidins have strong antimicrobial activity and immunomodulatory functions and are expected to be developed as substitutes for antibiotics. In this study, the antimicrobial activity of Hepc2 from Japanese seabass (<i>Lateolabrax japonicus</i>) (designated as LJ-hep2) was investigated using its recombinant precursor protein (rLJ-hep2) expressed in <i>Pichia pastoris</i> and a chemically synthesized mature peptide (LJ-hep2<sub>(66–86)</sub>). The results showed that both rLJ-hep2 and synthetic LJ-hep2<sub>(66–86)</sub> displayed broad antimicrobial spectrum with potent activity against gram-negative and gram-positive bacteria, and fungi. Especially, LJ-hep2<sub>(66–86)</sub> had stronger antimicrobial activity and exhibited potent activity against several clinically isolated multidrug-resistant bacteria, including <i>Acinetobacter baumannii</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i> and <i>Enterococcus faecium</i>. Moreover, LJ-hep2<sub>(66–86)</sub> exerted rapid bactericidal kinetic (killed tested bacteria within 2 h), induced significant morphological changes and promoted agglutination of <i>E. coli</i>, <i>P. aeruginosa</i> and <i>Aeromonas hydrophila</i>. The activity of LJ-hep2<sub>(66–86)</sub> against <i>E. coli</i>, <i>P. aeruginosa</i> and <i>A. hydrophila</i> was stable and remained active when heated for 30 min. In addition, LJ-hep2<sub>(66–86)</sub> exhibited no cytotoxicity to the mammalian cell line HEK293T and fish cell lines (EPC and ZF4). In vivo study showed that LJ-hep2<sub>(66–86)</sub> could improve the survival rate of marine medaka (<i>Oryzias melastigma</i>) by about 40% under the challenge of <i>A. hydrophila</i>, indicating its immunoprotective function. Taken together, both rLJ-hep2 and LJ-hep2<sub>(66–86)</sub> have good prospects to be used as potential antimicrobial agents in aquaculture and medicine in the future. |
| first_indexed | 2024-03-09T09:45:25Z |
| format | Article |
| id | doaj.art-0645bbd56b904588a22196210dc8fc03 |
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| language | English |
| last_indexed | 2024-03-09T09:45:25Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-0645bbd56b904588a22196210dc8fc032023-12-02T00:36:26ZengMDPI AGMarine Drugs1660-33972022-10-01201065110.3390/md20100651The Antimicrobial Peptide LJ-hep2 from <i>Lateolabrax japonicus</i> Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In VivoRuihao Gong0Zhe An1Weibin Zhang2Fangyi Chen3Ke-Jian Wang4State Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean & Earth Sciences, Xiamen University, Xiamen 361102, ChinaHepcidin is widely present in many kinds of fish and is an important innate immune factor. A variety of HAMP2-type hepcidins have strong antimicrobial activity and immunomodulatory functions and are expected to be developed as substitutes for antibiotics. In this study, the antimicrobial activity of Hepc2 from Japanese seabass (<i>Lateolabrax japonicus</i>) (designated as LJ-hep2) was investigated using its recombinant precursor protein (rLJ-hep2) expressed in <i>Pichia pastoris</i> and a chemically synthesized mature peptide (LJ-hep2<sub>(66–86)</sub>). The results showed that both rLJ-hep2 and synthetic LJ-hep2<sub>(66–86)</sub> displayed broad antimicrobial spectrum with potent activity against gram-negative and gram-positive bacteria, and fungi. Especially, LJ-hep2<sub>(66–86)</sub> had stronger antimicrobial activity and exhibited potent activity against several clinically isolated multidrug-resistant bacteria, including <i>Acinetobacter baumannii</i>, <i>Escherichia coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i> and <i>Enterococcus faecium</i>. Moreover, LJ-hep2<sub>(66–86)</sub> exerted rapid bactericidal kinetic (killed tested bacteria within 2 h), induced significant morphological changes and promoted agglutination of <i>E. coli</i>, <i>P. aeruginosa</i> and <i>Aeromonas hydrophila</i>. The activity of LJ-hep2<sub>(66–86)</sub> against <i>E. coli</i>, <i>P. aeruginosa</i> and <i>A. hydrophila</i> was stable and remained active when heated for 30 min. In addition, LJ-hep2<sub>(66–86)</sub> exhibited no cytotoxicity to the mammalian cell line HEK293T and fish cell lines (EPC and ZF4). In vivo study showed that LJ-hep2<sub>(66–86)</sub> could improve the survival rate of marine medaka (<i>Oryzias melastigma</i>) by about 40% under the challenge of <i>A. hydrophila</i>, indicating its immunoprotective function. Taken together, both rLJ-hep2 and LJ-hep2<sub>(66–86)</sub> have good prospects to be used as potential antimicrobial agents in aquaculture and medicine in the future.https://www.mdpi.com/1660-3397/20/10/651<i>Lateolabrax japonicus</i>hepcidinantimicrobial peptideantimicrobial activitymultidrug-resistant bacteriaimmunoprotective effect |
| spellingShingle | Ruihao Gong Zhe An Weibin Zhang Fangyi Chen Ke-Jian Wang The Antimicrobial Peptide LJ-hep2 from <i>Lateolabrax japonicus</i> Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo Marine Drugs <i>Lateolabrax japonicus</i> hepcidin antimicrobial peptide antimicrobial activity multidrug-resistant bacteria immunoprotective effect |
| title | The Antimicrobial Peptide LJ-hep2 from <i>Lateolabrax japonicus</i> Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo |
| title_full | The Antimicrobial Peptide LJ-hep2 from <i>Lateolabrax japonicus</i> Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo |
| title_fullStr | The Antimicrobial Peptide LJ-hep2 from <i>Lateolabrax japonicus</i> Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo |
| title_full_unstemmed | The Antimicrobial Peptide LJ-hep2 from <i>Lateolabrax japonicus</i> Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo |
| title_short | The Antimicrobial Peptide LJ-hep2 from <i>Lateolabrax japonicus</i> Exerting Activities against Multiple Pathogenic Bacteria and Immune Protection In Vivo |
| title_sort | antimicrobial peptide lj hep2 from i lateolabrax japonicus i exerting activities against multiple pathogenic bacteria and immune protection in vivo |
| topic | <i>Lateolabrax japonicus</i> hepcidin antimicrobial peptide antimicrobial activity multidrug-resistant bacteria immunoprotective effect |
| url | https://www.mdpi.com/1660-3397/20/10/651 |
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