Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro.
The culture conditions for the yield of a polysaccharide (PCPS) produced by Paecilomyces cicadae (Miquel) Samson on solid-state fermentation were investigated using response surface methodology (RSM). Plackett-Burman design (PBD) was applied to screen out significant factors, followed by the paths o...
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| Format: | Article |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3911990?pdf=render |
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| author | Xueyong Ren Liang He Junwen Cheng Jianmin Chang |
| author_facet | Xueyong Ren Liang He Junwen Cheng Jianmin Chang |
| author_sort | Xueyong Ren |
| collection | DOAJ |
| description | The culture conditions for the yield of a polysaccharide (PCPS) produced by Paecilomyces cicadae (Miquel) Samson on solid-state fermentation were investigated using response surface methodology (RSM). Plackett-Burman design (PBD) was applied to screen out significant factors, followed by the paths of steepest ascent to move to the nearest region of maximum response. Then Box-Behnken design (BBD) was conducted to optimize the final levels of the culture conditions. After analyzing the regression equation and the response surface contour plots, relative humidity 56.07%, inoculum 13.51 mL/100 g and temperature 27.09°C were found to be the optimal key parameters for PCPS production. The maximum predicted yield of PCPS was 10.76 mg/g under the optimized conditions. The resulting PCPS (FPCPS) generated at optimal conditions was purified by chromatography column and found to be composed of mannose (43.2%), rhamnose (32.1%), xylose (14.5%) and arabinose (10.2%). Based on the size exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS) analysis, FPCPS adopted a Gaussian coil conformation in 0.1 M NaNO3 solution with 3.75 × 10(6) g/mol of the weight-average molar mass (Mw) and 41.1 nm of the root-mean square radius (Rg(2))z (1/2). Furthermore, both of the polysaccharides were revealed to have strong antioxidant activities by evaluating in DPPH radical, superoxide radicals and hydroxyl radical assay. These data suggest the polysaccharides of Paecilomyces cicadae (Miquel) Samson produced by solid-state fermentation could be explored as potential natural antioxidants. |
| first_indexed | 2024-12-11T20:06:32Z |
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| id | doaj.art-0646823a56564b759dd9ca9e6ef426dd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T20:06:32Z |
| publishDate | 2014-01-01 |
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| spelling | doaj.art-0646823a56564b759dd9ca9e6ef426dd2022-12-22T00:52:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8757810.1371/journal.pone.0087578Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro.Xueyong RenLiang HeJunwen ChengJianmin ChangThe culture conditions for the yield of a polysaccharide (PCPS) produced by Paecilomyces cicadae (Miquel) Samson on solid-state fermentation were investigated using response surface methodology (RSM). Plackett-Burman design (PBD) was applied to screen out significant factors, followed by the paths of steepest ascent to move to the nearest region of maximum response. Then Box-Behnken design (BBD) was conducted to optimize the final levels of the culture conditions. After analyzing the regression equation and the response surface contour plots, relative humidity 56.07%, inoculum 13.51 mL/100 g and temperature 27.09°C were found to be the optimal key parameters for PCPS production. The maximum predicted yield of PCPS was 10.76 mg/g under the optimized conditions. The resulting PCPS (FPCPS) generated at optimal conditions was purified by chromatography column and found to be composed of mannose (43.2%), rhamnose (32.1%), xylose (14.5%) and arabinose (10.2%). Based on the size exclusion chromatography combined with multi-angle laser light scattering (SEC-MALLS) analysis, FPCPS adopted a Gaussian coil conformation in 0.1 M NaNO3 solution with 3.75 × 10(6) g/mol of the weight-average molar mass (Mw) and 41.1 nm of the root-mean square radius (Rg(2))z (1/2). Furthermore, both of the polysaccharides were revealed to have strong antioxidant activities by evaluating in DPPH radical, superoxide radicals and hydroxyl radical assay. These data suggest the polysaccharides of Paecilomyces cicadae (Miquel) Samson produced by solid-state fermentation could be explored as potential natural antioxidants.http://europepmc.org/articles/PMC3911990?pdf=render |
| spellingShingle | Xueyong Ren Liang He Junwen Cheng Jianmin Chang Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro. PLoS ONE |
| title | Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro. |
| title_full | Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro. |
| title_fullStr | Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro. |
| title_full_unstemmed | Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro. |
| title_short | Optimization of the solid-state fermentation and properties of a polysaccharide from Paecilomyces cicadae (Miquel) Samson and its antioxidant activities in vitro. |
| title_sort | optimization of the solid state fermentation and properties of a polysaccharide from paecilomyces cicadae miquel samson and its antioxidant activities in vitro |
| url | http://europepmc.org/articles/PMC3911990?pdf=render |
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