Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac Detection
Driven by the increasing concern about the risk of diclofenac (DCF) residues as water pollutants in the aqueous environment and the growing need for its trace determination, a simple but sensitive electrochemical aptasensor for the trace detection of DCF was developed. To construct the aptasensor, t...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-01-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2021.812909/full |
| _version_ | 1818976263505707008 |
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| author | Yi Zou Sophie Griveau Armelle Ringuedé Fethi Bedioui Cyrille Richard Cyrine Slim |
| author_facet | Yi Zou Sophie Griveau Armelle Ringuedé Fethi Bedioui Cyrille Richard Cyrine Slim |
| author_sort | Yi Zou |
| collection | DOAJ |
| description | Driven by the increasing concern about the risk of diclofenac (DCF) residues as water pollutants in the aqueous environment and the growing need for its trace determination, a simple but sensitive electrochemical aptasensor for the trace detection of DCF was developed. To construct the aptasensor, the amine-terminated DCF aptamer was covalently immobilized on the surface of the carboxylic acid–functionalized multi-walled carbon nanotube (f-MWCNT)–modified glassy carbon electrode (GCE) through EDC/NHS chemistry. The f-MWCNTs provide a reliable matrix for aptamer immobilization with high grafting density, while the aptamer serves as a biorecognition probe for DCF. The obtained aptasensor was incubated with DCF solutions at different concentrations and was then investigated by electrochemical impedance spectroscopy (EIS). It displays two linear ranges of concentration for DCF detection, from 250 fM to 1pM and from 1 pM to 500 nM with an extremely low detection limit of 162 fM. Also, the developed biosensor shows great reproducibility, acceptable stability, and reliable selectivity. Therefore, it offers a simple but effective aptasensor construction strategy for trace detection of DCF and is anticipated to show great potential for environmental applications. |
| first_indexed | 2024-12-20T16:09:04Z |
| format | Article |
| id | doaj.art-0648206cd3a64a09858616f9ceeb6c54 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-12-20T16:09:04Z |
| publishDate | 2022-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-0648206cd3a64a09858616f9ceeb6c542022-12-21T19:34:04ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462022-01-01910.3389/fchem.2021.812909812909Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac DetectionYi Zou0Sophie Griveau1Armelle Ringuedé2Fethi Bedioui3Cyrille Richard4Cyrine Slim5Institute of Chemistry for Life and Health Sciences (i-CLeHS), Chimie ParisTech, PSL Research University, CNRS, Paris, FranceInstitute of Chemistry for Life and Health Sciences (i-CLeHS), Chimie ParisTech, PSL Research University, CNRS, Paris, FranceInstitut de Recherche de Chimie de Paris (IRCP), PSL Research University, CNRS, Chimie ParisTech, Paris, FranceInstitute of Chemistry for Life and Health Sciences (i-CLeHS), Chimie ParisTech, PSL Research University, CNRS, Paris, FranceUniversité de Paris, CNRS, INSERM, UTCBS, Paris, FranceInstitute of Chemistry for Life and Health Sciences (i-CLeHS), Chimie ParisTech, PSL Research University, CNRS, Paris, FranceDriven by the increasing concern about the risk of diclofenac (DCF) residues as water pollutants in the aqueous environment and the growing need for its trace determination, a simple but sensitive electrochemical aptasensor for the trace detection of DCF was developed. To construct the aptasensor, the amine-terminated DCF aptamer was covalently immobilized on the surface of the carboxylic acid–functionalized multi-walled carbon nanotube (f-MWCNT)–modified glassy carbon electrode (GCE) through EDC/NHS chemistry. The f-MWCNTs provide a reliable matrix for aptamer immobilization with high grafting density, while the aptamer serves as a biorecognition probe for DCF. The obtained aptasensor was incubated with DCF solutions at different concentrations and was then investigated by electrochemical impedance spectroscopy (EIS). It displays two linear ranges of concentration for DCF detection, from 250 fM to 1pM and from 1 pM to 500 nM with an extremely low detection limit of 162 fM. Also, the developed biosensor shows great reproducibility, acceptable stability, and reliable selectivity. Therefore, it offers a simple but effective aptasensor construction strategy for trace detection of DCF and is anticipated to show great potential for environmental applications.https://www.frontiersin.org/articles/10.3389/fchem.2021.812909/fullelectrochemical aptasensoraptamerfunctionalized multi-walled carbon nanotubesdiclofenacimpedimetric detection |
| spellingShingle | Yi Zou Sophie Griveau Armelle Ringuedé Fethi Bedioui Cyrille Richard Cyrine Slim Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac Detection Frontiers in Chemistry electrochemical aptasensor aptamer functionalized multi-walled carbon nanotubes diclofenac impedimetric detection |
| title | Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac Detection |
| title_full | Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac Detection |
| title_fullStr | Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac Detection |
| title_full_unstemmed | Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac Detection |
| title_short | Functionalized Multi-Walled Carbon Nanotube–Based Aptasensors for Diclofenac Detection |
| title_sort | functionalized multi walled carbon nanotube based aptasensors for diclofenac detection |
| topic | electrochemical aptasensor aptamer functionalized multi-walled carbon nanotubes diclofenac impedimetric detection |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2021.812909/full |
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