Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT
Chronic allograft rejection is in part mediated by host T cells that recognize allogeneic antigens on transplanted tissue. One factor that determines the outcome of a T cell response is clonal size, while another is the effector quality. Studies of alloimmune predictors of transplant graft survival...
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| Format: | Article |
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MDPI AG
2012-05-01
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| Series: | Cells |
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| Online Access: | http://www.mdpi.com/2073-4409/1/2/127 |
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| author | Magdalena Tary-Lehmann Paul V. Lehmann Nicole L. Yonkers Benigno Rodriguez Kimberly A. Milkovich Wenji Zhang Donald D. Anthony |
| author_facet | Magdalena Tary-Lehmann Paul V. Lehmann Nicole L. Yonkers Benigno Rodriguez Kimberly A. Milkovich Wenji Zhang Donald D. Anthony |
| author_sort | Magdalena Tary-Lehmann |
| collection | DOAJ |
| description | Chronic allograft rejection is in part mediated by host T cells that recognize allogeneic antigens on transplanted tissue. One factor that determines the outcome of a T cell response is clonal size, while another is the effector quality. Studies of alloimmune predictors of transplant graft survival have most commonly focused on only one measure of the alloimmune response. Because differing qualities and frequencies of the allospecific T cell response may provide distinctly different information we analyzed the relationship between frequency of soluble antigen and allo-antigen specific memory IFN-g secreting CD4 and CD8 T cells, their ability to secrete IL-2, and their proliferative capacity, while accounting for cognate and bystander proliferation. The results show proliferative responses primarily reflect on IL-2 production by antigen-specific T cells, and that proliferating cells in such assays entail a considerable fraction of bystander cells. On the other hand, proliferation (and IL-2 production) did not reflect on the frequency of IFN-γ producing memory cells, a finding particularly accentuated in the CD8 T cell compartment. These data provide rationale for considering both frequency and effector function of pre-transplant T cell reactivity when analyzing immune predictors of graft rejection. |
| first_indexed | 2024-03-12T10:39:15Z |
| format | Article |
| id | doaj.art-064bd7db0e514726a8c12558b9b72b6e |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-12T10:39:15Z |
| publishDate | 2012-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-064bd7db0e514726a8c12558b9b72b6e2023-09-02T08:19:58ZengMDPI AGCells2073-44092012-05-011212714010.3390/cells1020127Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOTMagdalena Tary-LehmannPaul V. LehmannNicole L. YonkersBenigno RodriguezKimberly A. MilkovichWenji ZhangDonald D. AnthonyChronic allograft rejection is in part mediated by host T cells that recognize allogeneic antigens on transplanted tissue. One factor that determines the outcome of a T cell response is clonal size, while another is the effector quality. Studies of alloimmune predictors of transplant graft survival have most commonly focused on only one measure of the alloimmune response. Because differing qualities and frequencies of the allospecific T cell response may provide distinctly different information we analyzed the relationship between frequency of soluble antigen and allo-antigen specific memory IFN-g secreting CD4 and CD8 T cells, their ability to secrete IL-2, and their proliferative capacity, while accounting for cognate and bystander proliferation. The results show proliferative responses primarily reflect on IL-2 production by antigen-specific T cells, and that proliferating cells in such assays entail a considerable fraction of bystander cells. On the other hand, proliferation (and IL-2 production) did not reflect on the frequency of IFN-γ producing memory cells, a finding particularly accentuated in the CD8 T cell compartment. These data provide rationale for considering both frequency and effector function of pre-transplant T cell reactivity when analyzing immune predictors of graft rejection.http://www.mdpi.com/2073-4409/1/2/127T cellproliferationcytokineELISPOT |
| spellingShingle | Magdalena Tary-Lehmann Paul V. Lehmann Nicole L. Yonkers Benigno Rodriguez Kimberly A. Milkovich Wenji Zhang Donald D. Anthony Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT Cells T cell proliferation cytokine ELISPOT |
| title | Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT |
| title_full | Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT |
| title_fullStr | Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT |
| title_full_unstemmed | Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT |
| title_short | Dissecting the T Cell Response: Proliferation Assays vs. Cytokine Signatures by ELISPOT |
| title_sort | dissecting the t cell response proliferation assays vs cytokine signatures by elispot |
| topic | T cell proliferation cytokine ELISPOT |
| url | http://www.mdpi.com/2073-4409/1/2/127 |
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