Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage Polarization
Mesenchymal stem cells from bone marrow have powerful immunomodulatory capabilities. The interactions between jaw periosteal cells (JPCs) and macrophages are not only relevant for the application of JPCs in regenerative medicine, but this understanding could also help treating diseases like osteonec...
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MDPI AG
2021-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/9/4310 |
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| author | Fang He Felix Umrath Siegmar Reinert Dorothea Alexander |
| author_facet | Fang He Felix Umrath Siegmar Reinert Dorothea Alexander |
| author_sort | Fang He |
| collection | DOAJ |
| description | Mesenchymal stem cells from bone marrow have powerful immunomodulatory capabilities. The interactions between jaw periosteal cells (JPCs) and macrophages are not only relevant for the application of JPCs in regenerative medicine, but this understanding could also help treating diseases like osteonecrosis of the jaw. In previous studies, we analyzed, for the first time, immunomodulatory features of 2D- and 3D-cultured JPCs. In the present work, the effects of JPCs on the polarization state of macrophages in contact coculture were analyzed. To improve the macrophage polarization study, different concentrations of PMA (5 nM, 25 nM, and 150 nM) or different medium supplementations (10% FBS, 10% hPL and 5% hPL) were compared. Further, in order to analyze the effects of JPCs on macrophage polarization, JPCs and PMA-stimulated THP-1 cells were cocultured under LPS/IFN-γ or IL-4/IL-13 stimulatory conditions. Surface marker expression of M1 and M2 macrophages were analyzed under the different culture supplementations in order to investigate the immunomodulatory properties of JPCs. Our results showed that 5 nM PMA can conduct an effective macrophage polarization. The analyses of morphological parameters and surface marker expression showed more distinct M1/M2 phenotypes over FBS supplementation when using 5% hPL during macrophage polarization. In the coculture, immunomodulatory properties of JPCs improved significantly under 5% hPL supplementation compared to other supplementations. We concluded that, under the culture condition with 5% hPL, JPCs were able to effectively induce THP-1-derived macrophage polarization. |
| first_indexed | 2024-03-10T12:06:19Z |
| format | Article |
| id | doaj.art-064cdfdc67a04d6cbfc2d009afc7c20e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:06:19Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-064cdfdc67a04d6cbfc2d009afc7c20e2023-11-21T16:29:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01229431010.3390/ijms22094310Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage PolarizationFang He0Felix Umrath1Siegmar Reinert2Dorothea Alexander3Department of Oral and Maxillofacial Surgery, University Hospital Tübingen, 72076 Tübingen, GermanyDepartment of Oral and Maxillofacial Surgery, University Hospital Tübingen, 72076 Tübingen, GermanyDepartment of Oral and Maxillofacial Surgery, University Hospital Tübingen, 72076 Tübingen, GermanyDepartment of Oral and Maxillofacial Surgery, University Hospital Tübingen, 72076 Tübingen, GermanyMesenchymal stem cells from bone marrow have powerful immunomodulatory capabilities. The interactions between jaw periosteal cells (JPCs) and macrophages are not only relevant for the application of JPCs in regenerative medicine, but this understanding could also help treating diseases like osteonecrosis of the jaw. In previous studies, we analyzed, for the first time, immunomodulatory features of 2D- and 3D-cultured JPCs. In the present work, the effects of JPCs on the polarization state of macrophages in contact coculture were analyzed. To improve the macrophage polarization study, different concentrations of PMA (5 nM, 25 nM, and 150 nM) or different medium supplementations (10% FBS, 10% hPL and 5% hPL) were compared. Further, in order to analyze the effects of JPCs on macrophage polarization, JPCs and PMA-stimulated THP-1 cells were cocultured under LPS/IFN-γ or IL-4/IL-13 stimulatory conditions. Surface marker expression of M1 and M2 macrophages were analyzed under the different culture supplementations in order to investigate the immunomodulatory properties of JPCs. Our results showed that 5 nM PMA can conduct an effective macrophage polarization. The analyses of morphological parameters and surface marker expression showed more distinct M1/M2 phenotypes over FBS supplementation when using 5% hPL during macrophage polarization. In the coculture, immunomodulatory properties of JPCs improved significantly under 5% hPL supplementation compared to other supplementations. We concluded that, under the culture condition with 5% hPL, JPCs were able to effectively induce THP-1-derived macrophage polarization.https://www.mdpi.com/1422-0067/22/9/4310jaw periosteal cellsimmunomodulationmacrophage polarizationfetal bovine serumhuman platelet lysatecoculture |
| spellingShingle | Fang He Felix Umrath Siegmar Reinert Dorothea Alexander Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage Polarization International Journal of Molecular Sciences jaw periosteal cells immunomodulation macrophage polarization fetal bovine serum human platelet lysate coculture |
| title | Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage Polarization |
| title_full | Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage Polarization |
| title_fullStr | Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage Polarization |
| title_full_unstemmed | Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage Polarization |
| title_short | Jaw Periosteum-Derived Mesenchymal Stem Cells Regulate THP-1-Derived Macrophage Polarization |
| title_sort | jaw periosteum derived mesenchymal stem cells regulate thp 1 derived macrophage polarization |
| topic | jaw periosteal cells immunomodulation macrophage polarization fetal bovine serum human platelet lysate coculture |
| url | https://www.mdpi.com/1422-0067/22/9/4310 |
| work_keys_str_mv | AT fanghe jawperiosteumderivedmesenchymalstemcellsregulatethp1derivedmacrophagepolarization AT felixumrath jawperiosteumderivedmesenchymalstemcellsregulatethp1derivedmacrophagepolarization AT siegmarreinert jawperiosteumderivedmesenchymalstemcellsregulatethp1derivedmacrophagepolarization AT dorotheaalexander jawperiosteumderivedmesenchymalstemcellsregulatethp1derivedmacrophagepolarization |