Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong
ABSTRACTClostridioides difficile infection (CDI) is a common cause of nosocomial diarrhea and can sometimes lead to pseudo-membranous colitis and toxic megacolon. We previously reported that the PCR ribotype 002 was a common C. difficile ribotype in Hong Kong that was associated with increased morta...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2020-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2020.1739564 |
| _version_ | 1827320737417920512 |
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| author | Ka Yi Kong Thomas N. Y. Kwong Hung Chan Kristine Wong Samuel S. Y. Wong Anu P. Chaparala Raphael C. Y. Chan Lin Zhang Joseph J. Y. Sung Jun Yu Peter M. Hawkey Margaret Ip William K. K. Wu Sunny H. Wong |
| author_facet | Ka Yi Kong Thomas N. Y. Kwong Hung Chan Kristine Wong Samuel S. Y. Wong Anu P. Chaparala Raphael C. Y. Chan Lin Zhang Joseph J. Y. Sung Jun Yu Peter M. Hawkey Margaret Ip William K. K. Wu Sunny H. Wong |
| author_sort | Ka Yi Kong |
| collection | DOAJ |
| description | ABSTRACTClostridioides difficile infection (CDI) is a common cause of nosocomial diarrhea and can sometimes lead to pseudo-membranous colitis and toxic megacolon. We previously reported that the PCR ribotype 002 was a common C. difficile ribotype in Hong Kong that was associated with increased mortality. In this study, we assessed in vitro bacteriological characteristics and in vivo virulence of ribotype 002 compared to other common ribotypes, including ribotypes 012, 014 and 046. We observed significantly higher toxin A (p < 0.05) and toxin B (p < 0.05) production, sporulation (p < 0.001) and germination rates (p < 0.0001) in ribotype 002 than other common ribotypes. In a murine model of C. difficile infection, ribotype 002 caused significantly more weight loss (p < 0.001) and histological damage (p < 0.001) than other common ribotypes. These findings may have contributed to the higher prevalence and mortality observed, and provided mechanistic insights that can help public surveillance and develop novel therapeutics to combat against this infection. |
| first_indexed | 2024-03-07T17:24:45Z |
| format | Article |
| id | doaj.art-064d86e13f41488ba6c118bd21e19b70 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-04-25T00:51:15Z |
| publishDate | 2020-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-064d86e13f41488ba6c118bd21e19b702024-03-11T16:04:22ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512020-01-019163163810.1080/22221751.2020.1739564Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong KongKa Yi Kong0Thomas N. Y. Kwong1Hung Chan2Kristine Wong3Samuel S. Y. Wong4Anu P. Chaparala5Raphael C. Y. Chan6Lin Zhang7Joseph J. Y. Sung8Jun Yu9Peter M. Hawkey10Margaret Ip11William K. K. Wu12Sunny H. Wong13Institute of Digestive Disease, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARInstitute of Digestive Disease, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARDepartment of Anaesthesia and Intensive Care, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARDivision of Biological Sciences, University of California San Diego, San Diego, CA, USAInstitute of Digestive Disease, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARDivision of Biological Sciences, University of California San Diego, San Diego, CA, USADepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong SARDepartment of Anaesthesia and Intensive Care, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARInstitute of Digestive Disease, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARInstitute of Digestive Disease, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARInstitute of Microbiology and Infection, School of Biosciences, University of Birmingham, Birmingham, UKDepartment of Microbiology, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARState Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARInstitute of Digestive Disease, Department of Medicine and Therapeutics, The Chinese University of Hong Kong, Hong Kong, Hong Kong SARABSTRACTClostridioides difficile infection (CDI) is a common cause of nosocomial diarrhea and can sometimes lead to pseudo-membranous colitis and toxic megacolon. We previously reported that the PCR ribotype 002 was a common C. difficile ribotype in Hong Kong that was associated with increased mortality. In this study, we assessed in vitro bacteriological characteristics and in vivo virulence of ribotype 002 compared to other common ribotypes, including ribotypes 012, 014 and 046. We observed significantly higher toxin A (p < 0.05) and toxin B (p < 0.05) production, sporulation (p < 0.001) and germination rates (p < 0.0001) in ribotype 002 than other common ribotypes. In a murine model of C. difficile infection, ribotype 002 caused significantly more weight loss (p < 0.001) and histological damage (p < 0.001) than other common ribotypes. These findings may have contributed to the higher prevalence and mortality observed, and provided mechanistic insights that can help public surveillance and develop novel therapeutics to combat against this infection.https://www.tandfonline.com/doi/10.1080/22221751.2020.1739564Clostridioides difficileribotypesporulationgerminationtoxins |
| spellingShingle | Ka Yi Kong Thomas N. Y. Kwong Hung Chan Kristine Wong Samuel S. Y. Wong Anu P. Chaparala Raphael C. Y. Chan Lin Zhang Joseph J. Y. Sung Jun Yu Peter M. Hawkey Margaret Ip William K. K. Wu Sunny H. Wong Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong Emerging Microbes and Infections Clostridioides difficile ribotype sporulation germination toxins |
| title | Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong |
| title_full | Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong |
| title_fullStr | Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong |
| title_full_unstemmed | Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong |
| title_short | Biological characteristics associated with virulence in Clostridioides difficile ribotype 002 in Hong Kong |
| title_sort | biological characteristics associated with virulence in clostridioides difficile ribotype 002 in hong kong |
| topic | Clostridioides difficile ribotype sporulation germination toxins |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2020.1739564 |
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