Effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in rats
Objective To observe the injury of intestinal mucosal barrier and the expression of autophagy protein in colon tissue of rats with chronic renal failure. Methods Thirty SPF healthy male SD rats were randomly divided into sham operation group (10 rats) and model group (20 rats). The model group was g...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2022-10-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-10-1487.pdf |
| _version_ | 1827390628298752000 |
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| author | ZHANG Zi-yuan, FANG Jing-ai, LI Su-fen, HU Ya-ling, ZHANG Xiao-dong, LIU Wen-yuan, LIU Xue-jun |
| author_facet | ZHANG Zi-yuan, FANG Jing-ai, LI Su-fen, HU Ya-ling, ZHANG Xiao-dong, LIU Wen-yuan, LIU Xue-jun |
| author_sort | ZHANG Zi-yuan, FANG Jing-ai, LI Su-fen, HU Ya-ling, ZHANG Xiao-dong, LIU Wen-yuan, LIU Xue-jun |
| collection | DOAJ |
| description | Objective To observe the injury of intestinal mucosal barrier and the expression of autophagy protein in colon tissue of rats with chronic renal failure. Methods Thirty SPF healthy male SD rats were randomly divided into sham operation group (10 rats) and model group (20 rats). The model group was given adenine 200 mg/(kg·d) by gavage for 4 weeks after left nephrectomy. The general conditions of rats were observed. Serum, renal tissue and colon tissue samples were collected at 4 and 8 weeks after gavage. The levels of serum creatinine, urea nitrogen, TNF-α, IL-6 and LPS were measured by ELISA. Renal and colon tissue were examined by HE staining and microscopy. The protein expressions of LC3, beclin-1, ZO-1 and claudin-1 were detected by Western blot. Results Compared with the sham operation group, the model group rats gradually showed a slow response to external stimuli, slow action, withered and yellow hair color, dull luster, pale conjunctiva and slow weight gain. The levels of serum creatinine, urea nitrogen and inflammatory factors in the model group were all higher than those in the sham operation group, and increased at 8 weeks compared with 4 weeks (P＜0.05). Renal pathology in the model group showed renal tubular dilatation, lymphatic and monocyte infiltration, glomerulosclerosis and interstitial fibrosis. Histopathology of colon in model group showed atrophy and collapse of intestinal villi, infiltration of inflammatory cells in mucosal layer and aggravation of pathological changes, which was more serious at 8 weeks than at 4 weeks. The expression of LC3Ⅱ/Ⅰ, Beclin-1, ZO-1 and Claudin-1 in the model group decreased as compared to those of sham operation group(P＜0.05), and much decreased at 8 weeks as compared with 4 weeks (P＜0.05). Conclusions Autophagy disorder may be a potential mechanism causing intestinal mucosal barrier injury in rats with chronic renal failure. |
| first_indexed | 2024-03-08T16:54:32Z |
| format | Article |
| id | doaj.art-064de51634f94388af94a642cdc21c1d |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:54:32Z |
| publishDate | 2022-10-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-064de51634f94388af94a642cdc21c1d2024-01-05T02:36:29ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252022-10-0142101487149110.16352/j.issn.1001-6325.2022.10.1487Effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in ratsZHANG Zi-yuan, FANG Jing-ai, LI Su-fen, HU Ya-ling, ZHANG Xiao-dong, LIU Wen-yuan, LIU Xue-jun01. Department of Nephrology; 2. Department of Gynaecology; 3. Department of Geriatrics, the First Hospital of Shanxi Medical University, Taiyuan 030001; ;4. Shanxi Medical University, Taiyuan 030001, ChinaObjective To observe the injury of intestinal mucosal barrier and the expression of autophagy protein in colon tissue of rats with chronic renal failure. Methods Thirty SPF healthy male SD rats were randomly divided into sham operation group (10 rats) and model group (20 rats). The model group was given adenine 200 mg/(kg·d) by gavage for 4 weeks after left nephrectomy. The general conditions of rats were observed. Serum, renal tissue and colon tissue samples were collected at 4 and 8 weeks after gavage. The levels of serum creatinine, urea nitrogen, TNF-α, IL-6 and LPS were measured by ELISA. Renal and colon tissue were examined by HE staining and microscopy. The protein expressions of LC3, beclin-1, ZO-1 and claudin-1 were detected by Western blot. Results Compared with the sham operation group, the model group rats gradually showed a slow response to external stimuli, slow action, withered and yellow hair color, dull luster, pale conjunctiva and slow weight gain. The levels of serum creatinine, urea nitrogen and inflammatory factors in the model group were all higher than those in the sham operation group, and increased at 8 weeks compared with 4 weeks (P＜0.05). Renal pathology in the model group showed renal tubular dilatation, lymphatic and monocyte infiltration, glomerulosclerosis and interstitial fibrosis. Histopathology of colon in model group showed atrophy and collapse of intestinal villi, infiltration of inflammatory cells in mucosal layer and aggravation of pathological changes, which was more serious at 8 weeks than at 4 weeks. The expression of LC3Ⅱ/Ⅰ, Beclin-1, ZO-1 and Claudin-1 in the model group decreased as compared to those of sham operation group(P＜0.05), and much decreased at 8 weeks as compared with 4 weeks (P＜0.05). Conclusions Autophagy disorder may be a potential mechanism causing intestinal mucosal barrier injury in rats with chronic renal failure.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-10-1487.pdfautophagy|chronic renal failure|intestinal mucosal barrier |
| spellingShingle | ZHANG Zi-yuan, FANG Jing-ai, LI Su-fen, HU Ya-ling, ZHANG Xiao-dong, LIU Wen-yuan, LIU Xue-jun Effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in rats Jichu yixue yu linchuang autophagy|chronic renal failure|intestinal mucosal barrier |
| title | Effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in rats |
| title_full | Effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in rats |
| title_fullStr | Effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in rats |
| title_full_unstemmed | Effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in rats |
| title_short | Effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in rats |
| title_sort | effects of chronic renal failure on intestinal mucosal barrier and expression of autophagy related proteins in rats |
| topic | autophagy|chronic renal failure|intestinal mucosal barrier |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2022-42-10-1487.pdf |
| work_keys_str_mv | AT zhangziyuanfangjingailisufenhuyalingzhangxiaodongliuwenyuanliuxuejun effectsofchronicrenalfailureonintestinalmucosalbarrierandexpressionofautophagyrelatedproteinsinrats |