The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling Cattle
The purpose of this study was to analyze the transcriptome of <i>MyoD1</i> gene knockout MDBK cells (bovine kidney cells) using high-throughput sequencing. For the first time, CRISPR/CAS9 technology was used to construct a <i>MyoD1</i> knockout in MDBK cells and transcriptome...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/19/2571 |
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| author | Di Zhou Yan Wang Rong Yang Fu Wang Zhonghai Zhao Xin Wang Lingling Xie Xingzhou Tian Guoze Wang Bo Li Yu Gong |
| author_facet | Di Zhou Yan Wang Rong Yang Fu Wang Zhonghai Zhao Xin Wang Lingling Xie Xingzhou Tian Guoze Wang Bo Li Yu Gong |
| author_sort | Di Zhou |
| collection | DOAJ |
| description | The purpose of this study was to analyze the transcriptome of <i>MyoD1</i> gene knockout MDBK cells (bovine kidney cells) using high-throughput sequencing. For the first time, CRISPR/CAS9 technology was used to construct a <i>MyoD1</i> knockout in MDBK cells and transcriptome sequence analysis was used to examine <i>MyoD1</i>-related target gene expression. Transcriptome sequencing indicated the presence of 723 differentially expressed genes (DEGs) by comparing wild type and <i>MyoD1</i> knockout MDBK cells and included 178 upregulated and 72 downregulated genes. The DEGs are mainly enriched in Pl-3-kinase and AKT, p53 signaling pathways. Quantitative RT-PCR confirmed that <i>PDE1B</i>, <i>ADAMTS1</i>, <i>DPT,</i> and <i>CCND2</i> were highly expressed in the leg muscle, longissimus dorsi, and shoulder of Guanling cattle, and <i>CCND2</i> was inhibited after <i>MyoD1</i> knockout, suggesting it may be a key downstream gene of <i>MyoD1</i> and associated with muscle formation and differentiation in Guanling cattle. This provides experimental data for subsequent studies on the regulatory mechanisms of muscle differentiation in Guanling cattle. |
| first_indexed | 2024-03-09T22:08:04Z |
| format | Article |
| id | doaj.art-064e4e775a504c6baccdc9840cc7e664 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:08:04Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-064e4e775a504c6baccdc9840cc7e6642023-11-23T19:35:55ZengMDPI AGAnimals2076-26152022-09-011219257110.3390/ani12192571The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling CattleDi Zhou0Yan Wang1Rong Yang2Fu Wang3Zhonghai Zhao4Xin Wang5Lingling Xie6Xingzhou Tian7Guoze Wang8Bo Li9Yu Gong10Guizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, ChinaGuizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, ChinaGuizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, ChinaGuizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, ChinaGuizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, ChinaGuizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, ChinaGuizhou Testing Center for Livestock and Poultry Germplasm, Guiyang 550018, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, ChinaKey Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, ChinaGuizhou Livestock and Poultry Genetic Resources Management Station, Guiyang 550001, ChinaGuizhou Livestock and Poultry Genetic Resources Management Station, Guiyang 550001, ChinaThe purpose of this study was to analyze the transcriptome of <i>MyoD1</i> gene knockout MDBK cells (bovine kidney cells) using high-throughput sequencing. For the first time, CRISPR/CAS9 technology was used to construct a <i>MyoD1</i> knockout in MDBK cells and transcriptome sequence analysis was used to examine <i>MyoD1</i>-related target gene expression. Transcriptome sequencing indicated the presence of 723 differentially expressed genes (DEGs) by comparing wild type and <i>MyoD1</i> knockout MDBK cells and included 178 upregulated and 72 downregulated genes. The DEGs are mainly enriched in Pl-3-kinase and AKT, p53 signaling pathways. Quantitative RT-PCR confirmed that <i>PDE1B</i>, <i>ADAMTS1</i>, <i>DPT,</i> and <i>CCND2</i> were highly expressed in the leg muscle, longissimus dorsi, and shoulder of Guanling cattle, and <i>CCND2</i> was inhibited after <i>MyoD1</i> knockout, suggesting it may be a key downstream gene of <i>MyoD1</i> and associated with muscle formation and differentiation in Guanling cattle. This provides experimental data for subsequent studies on the regulatory mechanisms of muscle differentiation in Guanling cattle.https://www.mdpi.com/2076-2615/12/19/2571Guanling cattleCRISPR/CAS9transcriptome<i>MyoD1</i> |
| spellingShingle | Di Zhou Yan Wang Rong Yang Fu Wang Zhonghai Zhao Xin Wang Lingling Xie Xingzhou Tian Guoze Wang Bo Li Yu Gong The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling Cattle Animals Guanling cattle CRISPR/CAS9 transcriptome <i>MyoD1</i> |
| title | The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling Cattle |
| title_full | The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling Cattle |
| title_fullStr | The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling Cattle |
| title_full_unstemmed | The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling Cattle |
| title_short | The <i>MyoD1</i> Promoted Muscle Differentiation and Generation by Activating <i>CCND2</i> in Guanling Cattle |
| title_sort | i myod1 i promoted muscle differentiation and generation by activating i ccnd2 i in guanling cattle |
| topic | Guanling cattle CRISPR/CAS9 transcriptome <i>MyoD1</i> |
| url | https://www.mdpi.com/2076-2615/12/19/2571 |
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