Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification
Abstract At a crime scene, investigators are faced with a multitude of traces. Among them, biological traces are of primary interest for the rapid genetic-based identification of individuals. “Touch DNA” consists of invisible biological traces left by the simple contact of a person’s skin with objec...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-45366-y |
| _version_ | 1797452556807438336 |
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| author | Mathilde Recipon Rémy Agniel Johanne Leroy-Dudal Thibaud Fritz Franck Carreiras Francis Hermitte Sylvain Hubac Olivier Gallet Sabrina Kellouche |
| author_facet | Mathilde Recipon Rémy Agniel Johanne Leroy-Dudal Thibaud Fritz Franck Carreiras Francis Hermitte Sylvain Hubac Olivier Gallet Sabrina Kellouche |
| author_sort | Mathilde Recipon |
| collection | DOAJ |
| description | Abstract At a crime scene, investigators are faced with a multitude of traces. Among them, biological traces are of primary interest for the rapid genetic-based identification of individuals. “Touch DNA” consists of invisible biological traces left by the simple contact of a person’s skin with objects. To date, these traces remain undetectable with the current methods available in the field. This study proposes a proof-of-concept for the original detection of touch DNA by targeting cell-derived fragments in addition to DNA. More specifically, adhesive-structure proteins (laminin, keratin) as well as carbohydrate patterns (mannose, galactose) have been detected with keratinocyte cells derived from a skin and fingermark touch-DNA model over two months in outdoor conditions. Better still, this combinatory detection strategy is compatible with DNA profiling. This proof-of-concept work paves the way for the optimization of tools that can detect touch DNA, which remains a real challenge in helping investigators and the delivery of justice. |
| first_indexed | 2024-03-09T15:10:24Z |
| format | Article |
| id | doaj.art-0650c5291924447fa101ff41629b3a2c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:10:24Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0650c5291924447fa101ff41629b3a2c2023-11-26T13:23:22ZengNature PortfolioScientific Reports2045-23222023-10-0113111510.1038/s41598-023-45366-yTargeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identificationMathilde Recipon0Rémy Agniel1Johanne Leroy-Dudal2Thibaud Fritz3Franck Carreiras4Francis Hermitte5Sylvain Hubac6Olivier Gallet7Sabrina Kellouche8Equipe de Recherche sur les Relations Matrice Extracellulaire‐Cellules, ERRMECe, (EA1391), Groupe Matrice Extracellulaire et Physiopathologie (MECuP), Institut des Matériaux, I-MAT (FD4122), CY Cergy Paris UniversitéEquipe de Recherche sur les Relations Matrice Extracellulaire‐Cellules, ERRMECe, (EA1391), Groupe Matrice Extracellulaire et Physiopathologie (MECuP), Institut des Matériaux, I-MAT (FD4122), CY Cergy Paris UniversitéEquipe de Recherche sur les Relations Matrice Extracellulaire‐Cellules, ERRMECe, (EA1391), Groupe Matrice Extracellulaire et Physiopathologie (MECuP), Institut des Matériaux, I-MAT (FD4122), CY Cergy Paris UniversitéInstitut de Recherche Criminelle de la Gendarmerie NationaleEquipe de Recherche sur les Relations Matrice Extracellulaire‐Cellules, ERRMECe, (EA1391), Groupe Matrice Extracellulaire et Physiopathologie (MECuP), Institut des Matériaux, I-MAT (FD4122), CY Cergy Paris UniversitéInstitut de Recherche Criminelle de la Gendarmerie NationaleInstitut de Recherche Criminelle de la Gendarmerie NationaleEquipe de Recherche sur les Relations Matrice Extracellulaire‐Cellules, ERRMECe, (EA1391), Groupe Matrice Extracellulaire et Physiopathologie (MECuP), Institut des Matériaux, I-MAT (FD4122), CY Cergy Paris UniversitéEquipe de Recherche sur les Relations Matrice Extracellulaire‐Cellules, ERRMECe, (EA1391), Groupe Matrice Extracellulaire et Physiopathologie (MECuP), Institut des Matériaux, I-MAT (FD4122), CY Cergy Paris UniversitéAbstract At a crime scene, investigators are faced with a multitude of traces. Among them, biological traces are of primary interest for the rapid genetic-based identification of individuals. “Touch DNA” consists of invisible biological traces left by the simple contact of a person’s skin with objects. To date, these traces remain undetectable with the current methods available in the field. This study proposes a proof-of-concept for the original detection of touch DNA by targeting cell-derived fragments in addition to DNA. More specifically, adhesive-structure proteins (laminin, keratin) as well as carbohydrate patterns (mannose, galactose) have been detected with keratinocyte cells derived from a skin and fingermark touch-DNA model over two months in outdoor conditions. Better still, this combinatory detection strategy is compatible with DNA profiling. This proof-of-concept work paves the way for the optimization of tools that can detect touch DNA, which remains a real challenge in helping investigators and the delivery of justice.https://doi.org/10.1038/s41598-023-45366-y |
| spellingShingle | Mathilde Recipon Rémy Agniel Johanne Leroy-Dudal Thibaud Fritz Franck Carreiras Francis Hermitte Sylvain Hubac Olivier Gallet Sabrina Kellouche Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification Scientific Reports |
| title | Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification |
| title_full | Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification |
| title_fullStr | Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification |
| title_full_unstemmed | Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification |
| title_short | Targeting cell-derived markers to improve the detection of invisible biological traces for the purpose of genetic-based criminal identification |
| title_sort | targeting cell derived markers to improve the detection of invisible biological traces for the purpose of genetic based criminal identification |
| url | https://doi.org/10.1038/s41598-023-45366-y |
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