Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environment
Research on the microbiota associated with marine invertebrates is important for understanding host physiology and the relationship between the host and the environment. In this study, the microbiota of the green mussel Perna viridis was characterized at the tissue scale using 16S rRNA gene high-thr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1366305/full |
| _version_ | 1797214728955625472 |
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| author | Liying Chen Liying Chen Dai Li Yawei Shen Yawei Shen Zhuo Li Huanhuan Hao Caihuan Ke Caihuan Ke Zhang Meng Danqing Feng |
| author_facet | Liying Chen Liying Chen Dai Li Yawei Shen Yawei Shen Zhuo Li Huanhuan Hao Caihuan Ke Caihuan Ke Zhang Meng Danqing Feng |
| author_sort | Liying Chen |
| collection | DOAJ |
| description | Research on the microbiota associated with marine invertebrates is important for understanding host physiology and the relationship between the host and the environment. In this study, the microbiota of the green mussel Perna viridis was characterized at the tissue scale using 16S rRNA gene high-throughput sequencing and compared with the microbiota of the surrounding environment. Different mussel tissues were sampled, along with two environmental samples (the mussel's attachment substratum and seawater). The results showed that the phyla Proteobacteria, Bacteroidetes, and Spirochaetae were dominant in mussel tissues. The bacterial community composition at the family level varied among the tissues of P. viridis. Although the microbiota of P. viridis clearly differed from that of the surrounding seawater, the composition and diversity of the microbial community of the foot and outer shell surface were similar to those of the substratum, indicating their close relationship with the substratum. KEGG prediction analysis indicated that the bacteria harbored by P. viridis were enriched in the degradation of aromatic compounds, osmoregulation, and carbohydrate oxidation and fermentation, processes that may be important in P. viridis physiology. Our study provides new insights into the tissue-scale characteristics of mussel microbiomes and the intricate connection between mussels and their environment. |
| first_indexed | 2024-04-24T11:18:48Z |
| format | Article |
| id | doaj.art-06513756a5994e42bd43ee28043804ac |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T11:18:48Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-06513756a5994e42bd43ee28043804ac2024-04-11T04:56:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-04-011510.3389/fmicb.2024.13663051366305Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environmentLiying Chen0Liying Chen1Dai Li2Yawei Shen3Yawei Shen4Zhuo Li5Huanhuan Hao6Caihuan Ke7Caihuan Ke8Zhang Meng9Danqing Feng10State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaChina Nuclear Power Engineering Co., Ltd, Beijing, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaChina Nuclear Power Engineering Co., Ltd, Beijing, ChinaState Key Laboratory of Mariculture Breeding, College of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaResearch on the microbiota associated with marine invertebrates is important for understanding host physiology and the relationship between the host and the environment. In this study, the microbiota of the green mussel Perna viridis was characterized at the tissue scale using 16S rRNA gene high-throughput sequencing and compared with the microbiota of the surrounding environment. Different mussel tissues were sampled, along with two environmental samples (the mussel's attachment substratum and seawater). The results showed that the phyla Proteobacteria, Bacteroidetes, and Spirochaetae were dominant in mussel tissues. The bacterial community composition at the family level varied among the tissues of P. viridis. Although the microbiota of P. viridis clearly differed from that of the surrounding seawater, the composition and diversity of the microbial community of the foot and outer shell surface were similar to those of the substratum, indicating their close relationship with the substratum. KEGG prediction analysis indicated that the bacteria harbored by P. viridis were enriched in the degradation of aromatic compounds, osmoregulation, and carbohydrate oxidation and fermentation, processes that may be important in P. viridis physiology. Our study provides new insights into the tissue-scale characteristics of mussel microbiomes and the intricate connection between mussels and their environment.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1366305/fullmusselPerna viridistissue-associated microbiota16S rRNA genemicrobiota-environment relationship |
| spellingShingle | Liying Chen Liying Chen Dai Li Yawei Shen Yawei Shen Zhuo Li Huanhuan Hao Caihuan Ke Caihuan Ke Zhang Meng Danqing Feng Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environment Frontiers in Microbiology mussel Perna viridis tissue-associated microbiota 16S rRNA gene microbiota-environment relationship |
| title | Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environment |
| title_full | Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environment |
| title_fullStr | Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environment |
| title_full_unstemmed | Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environment |
| title_short | Microbiota characterization of the green mussel Perna viridis at the tissue scale and its relationship with the environment |
| title_sort | microbiota characterization of the green mussel perna viridis at the tissue scale and its relationship with the environment |
| topic | mussel Perna viridis tissue-associated microbiota 16S rRNA gene microbiota-environment relationship |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1366305/full |
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