Effective Utilization of <i>Vaccinium virgatum</i> Aiton Stems as Functional Materials: Major Constituent Analysis and Bioactivity Evaluation
We previously reported that rabbit-eye blueberry (<i>Vaccinium virgatum</i> Aiton) leaves exhibit multiple functions. In this study, we evaluated whether <i>V. virgatum</i> stems can also be used as functional materials similar to leaves and clarified the major constituents a...
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MDPI AG
2022-02-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/4/568 |
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| author | Hisahiro Kai Kazuhiro Sugamoto Saki Toshima Yo Goto Takayuki Nakayama Kazuhiro Morishita Hisato Kunitake |
| author_facet | Hisahiro Kai Kazuhiro Sugamoto Saki Toshima Yo Goto Takayuki Nakayama Kazuhiro Morishita Hisato Kunitake |
| author_sort | Hisahiro Kai |
| collection | DOAJ |
| description | We previously reported that rabbit-eye blueberry (<i>Vaccinium virgatum</i> Aiton) leaves exhibit multiple functions. In this study, we evaluated whether <i>V. virgatum</i> stems can also be used as functional materials similar to leaves and clarified the major constituents and their biological activity (antioxidant activity and anti–adult T cell leukemia (ATL) activity). Water extracts of <i>V. virgatum</i> stems were separated into 19 fractions using a Diaion HP-20 open column. Sugars and organic acids were detected in the highly water-soluble fractions. Polyphenols and proanthocyanidin were detected in the hydrous methanol-soluble fractions. In biological activity evaluations, a difference in antioxidant activity was observed in the water-containing methanol-eluted fractions, and fractions exhibiting anti-ATL activity differed depending on cell type. These results suggest that blueberry stems, like leaves, are rich in polyphenols and exhibit antioxidant activity and inhibit ATL cell growth. In the future, aerial parts of blueberries, including stems and leaves, could be used as functional materials and/or medicinal resources. |
| first_indexed | 2024-03-09T21:11:40Z |
| format | Article |
| id | doaj.art-06555e9477d84a18ae5b848c0daf8b95 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T21:11:40Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-06555e9477d84a18ae5b848c0daf8b952023-11-23T21:43:31ZengMDPI AGPlants2223-77472022-02-0111456810.3390/plants11040568Effective Utilization of <i>Vaccinium virgatum</i> Aiton Stems as Functional Materials: Major Constituent Analysis and Bioactivity EvaluationHisahiro Kai0Kazuhiro Sugamoto1Saki Toshima2Yo Goto3Takayuki Nakayama4Kazuhiro Morishita5Hisato Kunitake6Department of Pharmaceutical Health Sciences, School of Pharmaceutical Sciences, Kyushu University of Health and Welfare, Nobeoka 882-8508, JapanDepartment of Applied Chemistry, Faculty of Engineering, University of Miyazaki, Miyazaki 889-2192, JapanDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, JapanBiolabo Co., Ltd., Kobe 650-0047, JapanBiolabo Co., Ltd., Kobe 650-0047, JapanDivision of Tumor and Cellular Biochemistry, Department of Medical Sciences, Faculty of Medicine, University of Miyazaki, Miyazaki 889-2192, JapanDepartment of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, JapanWe previously reported that rabbit-eye blueberry (<i>Vaccinium virgatum</i> Aiton) leaves exhibit multiple functions. In this study, we evaluated whether <i>V. virgatum</i> stems can also be used as functional materials similar to leaves and clarified the major constituents and their biological activity (antioxidant activity and anti–adult T cell leukemia (ATL) activity). Water extracts of <i>V. virgatum</i> stems were separated into 19 fractions using a Diaion HP-20 open column. Sugars and organic acids were detected in the highly water-soluble fractions. Polyphenols and proanthocyanidin were detected in the hydrous methanol-soluble fractions. In biological activity evaluations, a difference in antioxidant activity was observed in the water-containing methanol-eluted fractions, and fractions exhibiting anti-ATL activity differed depending on cell type. These results suggest that blueberry stems, like leaves, are rich in polyphenols and exhibit antioxidant activity and inhibit ATL cell growth. In the future, aerial parts of blueberries, including stems and leaves, could be used as functional materials and/or medicinal resources.https://www.mdpi.com/2223-7747/11/4/568<i>Vaccinium virgatum</i>stemproanthocyanidin (PAC)antioxidant activityadult T cell leukemia (ATL) |
| spellingShingle | Hisahiro Kai Kazuhiro Sugamoto Saki Toshima Yo Goto Takayuki Nakayama Kazuhiro Morishita Hisato Kunitake Effective Utilization of <i>Vaccinium virgatum</i> Aiton Stems as Functional Materials: Major Constituent Analysis and Bioactivity Evaluation Plants <i>Vaccinium virgatum</i> stem proanthocyanidin (PAC) antioxidant activity adult T cell leukemia (ATL) |
| title | Effective Utilization of <i>Vaccinium virgatum</i> Aiton Stems as Functional Materials: Major Constituent Analysis and Bioactivity Evaluation |
| title_full | Effective Utilization of <i>Vaccinium virgatum</i> Aiton Stems as Functional Materials: Major Constituent Analysis and Bioactivity Evaluation |
| title_fullStr | Effective Utilization of <i>Vaccinium virgatum</i> Aiton Stems as Functional Materials: Major Constituent Analysis and Bioactivity Evaluation |
| title_full_unstemmed | Effective Utilization of <i>Vaccinium virgatum</i> Aiton Stems as Functional Materials: Major Constituent Analysis and Bioactivity Evaluation |
| title_short | Effective Utilization of <i>Vaccinium virgatum</i> Aiton Stems as Functional Materials: Major Constituent Analysis and Bioactivity Evaluation |
| title_sort | effective utilization of i vaccinium virgatum i aiton stems as functional materials major constituent analysis and bioactivity evaluation |
| topic | <i>Vaccinium virgatum</i> stem proanthocyanidin (PAC) antioxidant activity adult T cell leukemia (ATL) |
| url | https://www.mdpi.com/2223-7747/11/4/568 |
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