Evaluation of the anticonvulsant activity of the essential oil of Myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxin
Objective: To evaluate the anticonvulsant effect of the essential oil of Myrothamnus moschatus (M. moschatus) in convulsion induced by pentylenetetrazole and picrotoxin in rodent models. Methods: The essential oil of the aerial parts of M. moschatus was extracted by steam distillation. Thereafter, i...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-06-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169116302970 |
| _version_ | 1819260694923575296 |
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| author | Emmanuel Randrianarivo Filippo Maggi Marcello Nicoletti Philippe Rasoanaivo |
| author_facet | Emmanuel Randrianarivo Filippo Maggi Marcello Nicoletti Philippe Rasoanaivo |
| author_sort | Emmanuel Randrianarivo |
| collection | DOAJ |
| description | Objective: To evaluate the anticonvulsant effect of the essential oil of Myrothamnus moschatus (M. moschatus) in convulsion induced by pentylenetetrazole and picrotoxin in rodent models.
Methods: The essential oil of the aerial parts of M. moschatus was extracted by steam distillation. Thereafter, it was injected subcutaneously to rats and mice at escalating doses (0.1–0.8 mL/kg). Ten minutes after drug injection, pentylenetetrazole was injected intraperitoneally to rats and picrotoxin was administered to mice by the same route. Diazepam served as the positive control. Every single animal was placed into transparent cage and observed for convulsive behavior for 30 min by using ordinary security cameras connected to a video recorder. Death occurring for a period of 24 h was also recorded.
Results: The essential oil at 0.8 mL/kg completely arrested the pentylenetetrazole-induced convulsion without any sedative effect and delayed its appearance at lower doses, but showed moderate activities on picrotoxin-induced convulsion. For the rats treated with pentylenetetrazole alone, the mortality was 100% within 1 h, but for the rats pre-treated with the essential oil, the mortality was 0%. For the mice treated with picrotoxin, the mortality rate was also 100%, while 20%–100% died in those that had been pre-treated with the oil.
Conclusions: The results confirmed at least partly the traditional uses of the smoke of M. moschatus for the management of convulsion, and implied that the essential oil may inhibit the convulsion by GABAergic neuromodulation. |
| first_indexed | 2024-12-23T19:29:59Z |
| format | Article |
| id | doaj.art-0658c8621b174cef9d41c9cd759b6084 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-23T19:29:59Z |
| publishDate | 2016-06-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-0658c8621b174cef9d41c9cd759b60842022-12-21T17:33:57ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912016-06-016650150510.1016/j.apjtb.2016.01.017Evaluation of the anticonvulsant activity of the essential oil of Myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxinEmmanuel Randrianarivo0Filippo Maggi1Marcello Nicoletti2Philippe Rasoanaivo3Malagasy Institute of Applied Research, Rakoto Ratsimamanga Foundation, Avarabohitra Itaosy, lot AVB 77, Antananarivo, MadagascarSchool of Pharmacy, University of Camerino, Camerino, ItalyDepartment of Environmental Biology, Sapienza University, Rome, ItalyMalagasy Institute of Applied Research, Rakoto Ratsimamanga Foundation, Avarabohitra Itaosy, lot AVB 77, Antananarivo, MadagascarObjective: To evaluate the anticonvulsant effect of the essential oil of Myrothamnus moschatus (M. moschatus) in convulsion induced by pentylenetetrazole and picrotoxin in rodent models. Methods: The essential oil of the aerial parts of M. moschatus was extracted by steam distillation. Thereafter, it was injected subcutaneously to rats and mice at escalating doses (0.1–0.8 mL/kg). Ten minutes after drug injection, pentylenetetrazole was injected intraperitoneally to rats and picrotoxin was administered to mice by the same route. Diazepam served as the positive control. Every single animal was placed into transparent cage and observed for convulsive behavior for 30 min by using ordinary security cameras connected to a video recorder. Death occurring for a period of 24 h was also recorded. Results: The essential oil at 0.8 mL/kg completely arrested the pentylenetetrazole-induced convulsion without any sedative effect and delayed its appearance at lower doses, but showed moderate activities on picrotoxin-induced convulsion. For the rats treated with pentylenetetrazole alone, the mortality was 100% within 1 h, but for the rats pre-treated with the essential oil, the mortality was 0%. For the mice treated with picrotoxin, the mortality rate was also 100%, while 20%–100% died in those that had been pre-treated with the oil. Conclusions: The results confirmed at least partly the traditional uses of the smoke of M. moschatus for the management of convulsion, and implied that the essential oil may inhibit the convulsion by GABAergic neuromodulation.http://www.sciencedirect.com/science/article/pii/S2221169116302970Myrothamnus moschatusEssential oilConvulsionsPentylenetetrazolePicrotoxin |
| spellingShingle | Emmanuel Randrianarivo Filippo Maggi Marcello Nicoletti Philippe Rasoanaivo Evaluation of the anticonvulsant activity of the essential oil of Myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxin Asian Pacific Journal of Tropical Biomedicine Myrothamnus moschatus Essential oil Convulsions Pentylenetetrazole Picrotoxin |
| title | Evaluation of the anticonvulsant activity of the essential oil of Myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxin |
| title_full | Evaluation of the anticonvulsant activity of the essential oil of Myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxin |
| title_fullStr | Evaluation of the anticonvulsant activity of the essential oil of Myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxin |
| title_full_unstemmed | Evaluation of the anticonvulsant activity of the essential oil of Myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxin |
| title_short | Evaluation of the anticonvulsant activity of the essential oil of Myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxin |
| title_sort | evaluation of the anticonvulsant activity of the essential oil of myrothamnus moschatus in convulsion induced by pentylenetetrazole and picrotoxin |
| topic | Myrothamnus moschatus Essential oil Convulsions Pentylenetetrazole Picrotoxin |
| url | http://www.sciencedirect.com/science/article/pii/S2221169116302970 |
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