pH Auto-Sustain-Based Fermentation Supports Efficient Gamma-Aminobutyric Acid Production by <i>Lactobacillus brevis</i> CD0817
Gamma-aminobutyric acid (GABA) plays a role in several physiological functions. GABA production by lactic acid bacteria has attracted considerable interest; however, there is need to improve production. This study aimed to develop a pH auto-sustain (PAS)-based GABA fermentation process for <i>...
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| Format: | Article |
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MDPI AG
2022-05-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/5/208 |
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| author | Mengya Jia Yisong Zhu Lingqin Wang Tianyi Sun Hao Pan Haixing Li |
| author_facet | Mengya Jia Yisong Zhu Lingqin Wang Tianyi Sun Hao Pan Haixing Li |
| author_sort | Mengya Jia |
| collection | DOAJ |
| description | Gamma-aminobutyric acid (GABA) plays a role in several physiological functions. GABA production by lactic acid bacteria has attracted considerable interest; however, there is need to improve production. This study aimed to develop a pH auto-sustain (PAS)-based GABA fermentation process for <i>Lactobacillus brevis</i> CD0817, with L-glutamic acid (solubility ~6.0 g/L and isoelectric point 3.22) as the substrate. Firstly, we determined the optimum levels of vital factors affecting GABA synthesis using Erlenmeyer flask experiments. The results showed that optimal levels of sugar, yeast extract, Tween-80, manganese ion, and temperature were 5.0 g/L, 35.0 g/L, 1.0 g/L, 16.0 mg/L, and 30.0 °C, respectively. The added L-glutamic acid (650 g per liter of medium) mostly existed in the form of solid powder was slowly released to supply the substrate and acidity essential for GABA production with the progress of fermentation. Based on the optimizations, the PAS-based GABA fermentation was performed using a 10 L fermenter. The PAS-based strategy promoted GABA synthesis by the strain of up to 321.9 ± 6.7 g/L after 48 h, with a productivity of 6.71 g/L/h and a substrate molar conversion rate of 99.6%. The findings suggest that the PAS-based fermentation is a promising method for GABA production by lactic acid bacteria. |
| first_indexed | 2024-03-10T03:54:45Z |
| format | Article |
| id | doaj.art-065984d86a5940d9a3cc9d068306e92a |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-10T03:54:45Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-065984d86a5940d9a3cc9d068306e92a2023-11-23T10:56:59ZengMDPI AGFermentation2311-56372022-05-018520810.3390/fermentation8050208pH Auto-Sustain-Based Fermentation Supports Efficient Gamma-Aminobutyric Acid Production by <i>Lactobacillus brevis</i> CD0817Mengya Jia0Yisong Zhu1Lingqin Wang2Tianyi Sun3Hao Pan4Haixing Li5State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaHangzhou Xiaoshan Agricultural Development Co., Ltd., Hangzhou 311200, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaGamma-aminobutyric acid (GABA) plays a role in several physiological functions. GABA production by lactic acid bacteria has attracted considerable interest; however, there is need to improve production. This study aimed to develop a pH auto-sustain (PAS)-based GABA fermentation process for <i>Lactobacillus brevis</i> CD0817, with L-glutamic acid (solubility ~6.0 g/L and isoelectric point 3.22) as the substrate. Firstly, we determined the optimum levels of vital factors affecting GABA synthesis using Erlenmeyer flask experiments. The results showed that optimal levels of sugar, yeast extract, Tween-80, manganese ion, and temperature were 5.0 g/L, 35.0 g/L, 1.0 g/L, 16.0 mg/L, and 30.0 °C, respectively. The added L-glutamic acid (650 g per liter of medium) mostly existed in the form of solid powder was slowly released to supply the substrate and acidity essential for GABA production with the progress of fermentation. Based on the optimizations, the PAS-based GABA fermentation was performed using a 10 L fermenter. The PAS-based strategy promoted GABA synthesis by the strain of up to 321.9 ± 6.7 g/L after 48 h, with a productivity of 6.71 g/L/h and a substrate molar conversion rate of 99.6%. The findings suggest that the PAS-based fermentation is a promising method for GABA production by lactic acid bacteria.https://www.mdpi.com/2311-5637/8/5/208gamma-aminobutyric acid<i>Lactobacillus brevis</i> CD0817culture condition optimizationpH auto-sustain-based GABA fermentation |
| spellingShingle | Mengya Jia Yisong Zhu Lingqin Wang Tianyi Sun Hao Pan Haixing Li pH Auto-Sustain-Based Fermentation Supports Efficient Gamma-Aminobutyric Acid Production by <i>Lactobacillus brevis</i> CD0817 Fermentation gamma-aminobutyric acid <i>Lactobacillus brevis</i> CD0817 culture condition optimization pH auto-sustain-based GABA fermentation |
| title | pH Auto-Sustain-Based Fermentation Supports Efficient Gamma-Aminobutyric Acid Production by <i>Lactobacillus brevis</i> CD0817 |
| title_full | pH Auto-Sustain-Based Fermentation Supports Efficient Gamma-Aminobutyric Acid Production by <i>Lactobacillus brevis</i> CD0817 |
| title_fullStr | pH Auto-Sustain-Based Fermentation Supports Efficient Gamma-Aminobutyric Acid Production by <i>Lactobacillus brevis</i> CD0817 |
| title_full_unstemmed | pH Auto-Sustain-Based Fermentation Supports Efficient Gamma-Aminobutyric Acid Production by <i>Lactobacillus brevis</i> CD0817 |
| title_short | pH Auto-Sustain-Based Fermentation Supports Efficient Gamma-Aminobutyric Acid Production by <i>Lactobacillus brevis</i> CD0817 |
| title_sort | ph auto sustain based fermentation supports efficient gamma aminobutyric acid production by i lactobacillus brevis i cd0817 |
| topic | gamma-aminobutyric acid <i>Lactobacillus brevis</i> CD0817 culture condition optimization pH auto-sustain-based GABA fermentation |
| url | https://www.mdpi.com/2311-5637/8/5/208 |
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