Validation of an automated assay for measurement of bovine plasma ceruloplasmin
Abstract Ceruloplasmin (Cp) plays an important role in copper transport and iron metabolism, as well as Cp is also an indicator for the health status of dairy cows. The present study reports the validation of an automated assay to assess the plasma Cp in dairy cows. Plasma Cp levels were determined...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2019-07-01
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| Series: | Acta Veterinaria Scandinavica |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13028-019-0470-4 |
| _version_ | 1831807910750978048 |
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| author | Hussein Awad Hussein Jacqueline Bäumer Rudolf Staufenbiel |
| author_facet | Hussein Awad Hussein Jacqueline Bäumer Rudolf Staufenbiel |
| author_sort | Hussein Awad Hussein |
| collection | DOAJ |
| description | Abstract Ceruloplasmin (Cp) plays an important role in copper transport and iron metabolism, as well as Cp is also an indicator for the health status of dairy cows. The present study reports the validation of an automated assay to assess the plasma Cp in dairy cows. Plasma Cp levels were determined in 40 Holstein cows and intra- and inter-assay precision, accuracy, detection limit, and clinical validation of the assay were determined. Intra- and inter-assay coefficients of variation were < 2% and < 7%, respectively. The results were linear when serial sample dilutions were tested (r = 0.999, P < 0.001). The detection limit was lower than what could be measured in plasma from healthy cows. Increased plasma Cp levels were found in cows with inflammatory diseases. The method validated in this study is precise, simple, and fast and can be easily adapted to biochemical automated analysers. Furthermore, the promising results obtained with this protein will contribute to a wider use of Cp determination in bovine practice. |
| first_indexed | 2024-12-22T20:13:38Z |
| format | Article |
| id | doaj.art-065acb7a9579436ba56e7f0bfbf0c578 |
| institution | Directory Open Access Journal |
| issn | 1751-0147 |
| language | English |
| last_indexed | 2024-12-22T20:13:38Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Acta Veterinaria Scandinavica |
| spelling | doaj.art-065acb7a9579436ba56e7f0bfbf0c5782022-12-21T18:14:01ZengBMCActa Veterinaria Scandinavica1751-01472019-07-016111510.1186/s13028-019-0470-4Validation of an automated assay for measurement of bovine plasma ceruloplasminHussein Awad Hussein0Jacqueline Bäumer1Rudolf Staufenbiel2Veterinary Internal Medicine, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut UniversityKlinik für Klauentiere, Freie Universität BerlinKlinik für Klauentiere, Freie Universität BerlinAbstract Ceruloplasmin (Cp) plays an important role in copper transport and iron metabolism, as well as Cp is also an indicator for the health status of dairy cows. The present study reports the validation of an automated assay to assess the plasma Cp in dairy cows. Plasma Cp levels were determined in 40 Holstein cows and intra- and inter-assay precision, accuracy, detection limit, and clinical validation of the assay were determined. Intra- and inter-assay coefficients of variation were < 2% and < 7%, respectively. The results were linear when serial sample dilutions were tested (r = 0.999, P < 0.001). The detection limit was lower than what could be measured in plasma from healthy cows. Increased plasma Cp levels were found in cows with inflammatory diseases. The method validated in this study is precise, simple, and fast and can be easily adapted to biochemical automated analysers. Furthermore, the promising results obtained with this protein will contribute to a wider use of Cp determination in bovine practice.http://link.springer.com/article/10.1186/s13028-019-0470-4AnalyticalAutomaticCeruloplasminCowsMeasurementValidation |
| spellingShingle | Hussein Awad Hussein Jacqueline Bäumer Rudolf Staufenbiel Validation of an automated assay for measurement of bovine plasma ceruloplasmin Acta Veterinaria Scandinavica Analytical Automatic Ceruloplasmin Cows Measurement Validation |
| title | Validation of an automated assay for measurement of bovine plasma ceruloplasmin |
| title_full | Validation of an automated assay for measurement of bovine plasma ceruloplasmin |
| title_fullStr | Validation of an automated assay for measurement of bovine plasma ceruloplasmin |
| title_full_unstemmed | Validation of an automated assay for measurement of bovine plasma ceruloplasmin |
| title_short | Validation of an automated assay for measurement of bovine plasma ceruloplasmin |
| title_sort | validation of an automated assay for measurement of bovine plasma ceruloplasmin |
| topic | Analytical Automatic Ceruloplasmin Cows Measurement Validation |
| url | http://link.springer.com/article/10.1186/s13028-019-0470-4 |
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