Ocular hypotensive effect of fermented Pentaclethra macrophylla seeds in experimentally-induced glaucoma
The study aimed to investigate the effect of fermented n-hexane extract of Pentaclethra macrophylla seeds (FPMSE) in an experimental model of glaucoma. Eighteen rabbits (1-1.8 kg body weight) were allocated into six groups of three rabbits (six eyes per group). Group 1 was the normal control. Ocular...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | All Life |
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| Online Access: | http://dx.doi.org/10.1080/26895293.2023.2200578 |
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| author | Ikechukwu Jacob Okoro Parker Elijah Joshua Samuel Kyei Innocent Uzochukwu Okagu Christian Chijioke Amah Vitus Ikenna Nnamani Victor Nwadiogbu Ogugua |
| author_facet | Ikechukwu Jacob Okoro Parker Elijah Joshua Samuel Kyei Innocent Uzochukwu Okagu Christian Chijioke Amah Vitus Ikenna Nnamani Victor Nwadiogbu Ogugua |
| author_sort | Ikechukwu Jacob Okoro |
| collection | DOAJ |
| description | The study aimed to investigate the effect of fermented n-hexane extract of Pentaclethra macrophylla seeds (FPMSE) in an experimental model of glaucoma. Eighteen rabbits (1-1.8 kg body weight) were allocated into six groups of three rabbits (six eyes per group). Group 1 was the normal control. Ocular hypertension was induced with 1% prednisolone acetate ophthalmic solution in groups 2-6, twice daily for 21 days. Group 2 received no treatment, while groups 3–6 received 5 mg/kg b.w. acetazolamide, 100, 200, and 300 mg/kg b.w. extract, respectively. The intraocular pressures and other biochemical parameters were evaluated. The existence of bioactive compounds was determined via phytochemical analysis. Compared to group 2, the administration of FPMSE resulted in a significant decrease in IOP in the treated groups. Malondialdehyde concentrations in the treated groups’ aqueous humor and plasma were considerably lower (p < 0.05) than in group 2. Compared to group 2, glutathione, nitric oxide, and antioxidant levels improved significantly (p < 0.05). C-reactive protein and glutamate levels in group 2 were comparable (p > 0.05). FPMSE substantially reduced phospholipase A2 activity. Histological assessment of the ciliary body showed adequate pigmentations in the treatment groups compared to group 2. Hence, FPMSE possesses ocular hypotensive effects. |
| first_indexed | 2024-03-09T02:08:25Z |
| format | Article |
| id | doaj.art-065b75fb6d0f43fea272bc04d9c91e16 |
| institution | Directory Open Access Journal |
| issn | 2689-5307 |
| language | English |
| last_indexed | 2024-03-09T02:08:25Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | All Life |
| spelling | doaj.art-065b75fb6d0f43fea272bc04d9c91e162023-12-07T15:12:04ZengTaylor & Francis GroupAll Life2689-53072023-12-0116110.1080/26895293.2023.22005782200578Ocular hypotensive effect of fermented Pentaclethra macrophylla seeds in experimentally-induced glaucomaIkechukwu Jacob Okoro0Parker Elijah Joshua1Samuel Kyei2Innocent Uzochukwu Okagu3Christian Chijioke Amah4Vitus Ikenna Nnamani5Victor Nwadiogbu Ogugua6University of NigeriaUniversity of NigeriaUniversity of Cape-CoastUniversity of NigeriaUniversity of NigeriaUniversity of NigeriaUniversity of NigeriaThe study aimed to investigate the effect of fermented n-hexane extract of Pentaclethra macrophylla seeds (FPMSE) in an experimental model of glaucoma. Eighteen rabbits (1-1.8 kg body weight) were allocated into six groups of three rabbits (six eyes per group). Group 1 was the normal control. Ocular hypertension was induced with 1% prednisolone acetate ophthalmic solution in groups 2-6, twice daily for 21 days. Group 2 received no treatment, while groups 3–6 received 5 mg/kg b.w. acetazolamide, 100, 200, and 300 mg/kg b.w. extract, respectively. The intraocular pressures and other biochemical parameters were evaluated. The existence of bioactive compounds was determined via phytochemical analysis. Compared to group 2, the administration of FPMSE resulted in a significant decrease in IOP in the treated groups. Malondialdehyde concentrations in the treated groups’ aqueous humor and plasma were considerably lower (p < 0.05) than in group 2. Compared to group 2, glutathione, nitric oxide, and antioxidant levels improved significantly (p < 0.05). C-reactive protein and glutamate levels in group 2 were comparable (p > 0.05). FPMSE substantially reduced phospholipase A2 activity. Histological assessment of the ciliary body showed adequate pigmentations in the treatment groups compared to group 2. Hence, FPMSE possesses ocular hypotensive effects.http://dx.doi.org/10.1080/26895293.2023.2200578pentaclethra macrophyllaintraocular pressuresteroid-induced glaucomaaqueous humoracetazolamide |
| spellingShingle | Ikechukwu Jacob Okoro Parker Elijah Joshua Samuel Kyei Innocent Uzochukwu Okagu Christian Chijioke Amah Vitus Ikenna Nnamani Victor Nwadiogbu Ogugua Ocular hypotensive effect of fermented Pentaclethra macrophylla seeds in experimentally-induced glaucoma All Life pentaclethra macrophylla intraocular pressure steroid-induced glaucoma aqueous humor acetazolamide |
| title | Ocular hypotensive effect of fermented Pentaclethra macrophylla seeds in experimentally-induced glaucoma |
| title_full | Ocular hypotensive effect of fermented Pentaclethra macrophylla seeds in experimentally-induced glaucoma |
| title_fullStr | Ocular hypotensive effect of fermented Pentaclethra macrophylla seeds in experimentally-induced glaucoma |
| title_full_unstemmed | Ocular hypotensive effect of fermented Pentaclethra macrophylla seeds in experimentally-induced glaucoma |
| title_short | Ocular hypotensive effect of fermented Pentaclethra macrophylla seeds in experimentally-induced glaucoma |
| title_sort | ocular hypotensive effect of fermented pentaclethra macrophylla seeds in experimentally induced glaucoma |
| topic | pentaclethra macrophylla intraocular pressure steroid-induced glaucoma aqueous humor acetazolamide |
| url | http://dx.doi.org/10.1080/26895293.2023.2200578 |
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