ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS
The current study was undertaken to immobilize Kluveromyces lactis β-galactosidase on alginate coated magnesium oxide nanoparticles (ACMONPs). Transmission electron microscopy showed that MgO-NPs synthesized by wet chemical approach were of 27 nm size and spherical in shape. Excellent biocompatibili...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2023-10-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000900867&tlng=en |
| _version_ | 1827778349013925888 |
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| author | Shakeel Ahmed Ansari Rukhsana Satar Nadeem Ikram Muhammad Awais |
| author_facet | Shakeel Ahmed Ansari Rukhsana Satar Nadeem Ikram Muhammad Awais |
| author_sort | Shakeel Ahmed Ansari |
| collection | DOAJ |
| description | The current study was undertaken to immobilize Kluveromyces lactis β-galactosidase on alginate coated magnesium oxide nanoparticles (ACMONPs). Transmission electron microscopy showed that MgO-NPs synthesized by wet chemical approach were of 27 nm size and spherical in shape. Excellent biocompatibility of sodium alginate resulted in 90% immobilization yield for β-galactosidase due to covalent attachment. Soluble and immobilized β-galactosidase exhibited its pH and temperature-optima at pH 7.0 and at 40 °C, respectively. However, the enzyme attached to ACMONPs enhanced its activity at higher and lower pH and temperature ranges, in contrast to enzyme in solution. ACMONPs bound enzyme displayed greater enzyme activity under the effect of galactose mediated product inhibition as well as in reusability experiment. Our study indicated that 88% lactose was hydrolyzed by β-galactosidase immobilized on ACMONPs at 40 °C as compared to 71% by soluble enzyme under identical temperature in controlled batch reactors. Hence, this nanomatrix can find its potential application in continuous packed-bed reactors for obtaining lactose-free dairy products. |
| first_indexed | 2024-03-11T14:31:47Z |
| format | Article |
| id | doaj.art-065b9ac503bd457c8747919cfdb25c39 |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-03-11T14:31:47Z |
| publishDate | 2023-10-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-065b9ac503bd457c8747919cfdb25c392023-10-31T07:44:41ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642023-10-0146986787210.21577/0100-4042.20230071ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONSShakeel Ahmed Ansarihttps://orcid.org/0000-0002-5630-8034Rukhsana SatarNadeem IkramMuhammad AwaisThe current study was undertaken to immobilize Kluveromyces lactis β-galactosidase on alginate coated magnesium oxide nanoparticles (ACMONPs). Transmission electron microscopy showed that MgO-NPs synthesized by wet chemical approach were of 27 nm size and spherical in shape. Excellent biocompatibility of sodium alginate resulted in 90% immobilization yield for β-galactosidase due to covalent attachment. Soluble and immobilized β-galactosidase exhibited its pH and temperature-optima at pH 7.0 and at 40 °C, respectively. However, the enzyme attached to ACMONPs enhanced its activity at higher and lower pH and temperature ranges, in contrast to enzyme in solution. ACMONPs bound enzyme displayed greater enzyme activity under the effect of galactose mediated product inhibition as well as in reusability experiment. Our study indicated that 88% lactose was hydrolyzed by β-galactosidase immobilized on ACMONPs at 40 °C as compared to 71% by soluble enzyme under identical temperature in controlled batch reactors. Hence, this nanomatrix can find its potential application in continuous packed-bed reactors for obtaining lactose-free dairy products.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000900867&tlng=enβ-galactosidasebiotechnology industrymagnesium nanoparticlessodium alginatesurface functionalization |
| spellingShingle | Shakeel Ahmed Ansari Rukhsana Satar Nadeem Ikram Muhammad Awais ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS Química Nova β-galactosidase biotechnology industry magnesium nanoparticles sodium alginate surface functionalization |
| title | ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS |
| title_full | ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS |
| title_fullStr | ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS |
| title_full_unstemmed | ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS |
| title_short | ALGINATE COATED MgO-NPs IMPROVES THE STABILITY OF Kluyveromyces lactis β-GALACTOSIDASE FOR BIOTECHNOLOGICAL APPLICATIONS |
| title_sort | alginate coated mgo nps improves the stability of kluyveromyces lactis β galactosidase for biotechnological applications |
| topic | β-galactosidase biotechnology industry magnesium nanoparticles sodium alginate surface functionalization |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422023000900867&tlng=en |
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