Comprehensive analysis of the prognostic and immunotherapeutic implications of STAT family members in human colorectal cancer
Background: Colorectal cancer (CRC) is the third most prevalent cancer worldwide and the second leading cause of cancer mortality. Signal transducer and activator of transcription (STAT) proteins are a group of transcription factors implicated in cell signal transduction and gene transcription in se...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.951252/full |
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| author | Dingchang Li Dingchang Li Yanan Jiao Yanan Jiao Wenxing Gao Wenxing Gao Shidong Hu Dingling Li Wen Zhao Peng Chen Peng Chen Lujia Jin Lujia Jin Yingjie Zhao Yingjie Zhao Zhaofu Ma Zhaofu Ma Xiansheng Wu Yang Yan Wen Sun Xiaohui Du Guanglong Dong Guanglong Dong |
| author_facet | Dingchang Li Dingchang Li Yanan Jiao Yanan Jiao Wenxing Gao Wenxing Gao Shidong Hu Dingling Li Wen Zhao Peng Chen Peng Chen Lujia Jin Lujia Jin Yingjie Zhao Yingjie Zhao Zhaofu Ma Zhaofu Ma Xiansheng Wu Yang Yan Wen Sun Xiaohui Du Guanglong Dong Guanglong Dong |
| author_sort | Dingchang Li |
| collection | DOAJ |
| description | Background: Colorectal cancer (CRC) is the third most prevalent cancer worldwide and the second leading cause of cancer mortality. Signal transducer and activator of transcription (STAT) proteins are a group of transcription factors implicated in cell signal transduction and gene transcription in several cancer types. However, the level of expression, genetic alterations, and biological function of different STATs, as well as their prognostic and immunotherapeutic value in CRC remain unclear.Methods: The mRNA and protein expression levels, genetic alterations, prognostic value, gene–gene and protein–protein interaction networks, and biological function of STATs in CRC were studied using the GEPIA, HPA, cBioPortal, PrognoScan, Kaplan–Meier plotter, GeneMANIA, STRING, and Metascape databases. The expression of STATs in CRC was confirmed using immunohistochemistry (IHC). Finally, the relationship between STAT expression and immune infiltration as well as immunotherapy-associated indicators was also investigated.Results: The expression levels of STAT2/5A/5B are downregulated in CRC, and the STAT1/3/4/5B expressions were significantly associated with the tumor stage of patients with CRC. The abnormal expression of STAT2/4/5B in patients with CRC is related to the prognosis of patients with CRC. The STATs and their neighboring proteins are primarily associated with lymphocyte activation, cytokine-mediated signaling pathways, positive regulation of immune response, regulation of cytokine production, and growth hormone receptor signaling pathways in cancer. The expression of STATs was significantly associated with immune infiltration and immunotherapy response-associated indicators.Conclusion: This study may help further understand the molecular mechanism of CRC and provide new prognostic biomarkers and immunotherapy targets in patients with CRC. |
| first_indexed | 2024-12-10T15:45:25Z |
| format | Article |
| id | doaj.art-065bc44733924f249d1bb27fed7d79c6 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-10T15:45:25Z |
| publishDate | 2022-08-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-065bc44733924f249d1bb27fed7d79c62022-12-22T01:42:57ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-08-011310.3389/fgene.2022.951252951252Comprehensive analysis of the prognostic and immunotherapeutic implications of STAT family members in human colorectal cancerDingchang Li0Dingchang Li1Yanan Jiao2Yanan Jiao3Wenxing Gao4Wenxing Gao5Shidong Hu6Dingling Li7Wen Zhao8Peng Chen9Peng Chen10Lujia Jin11Lujia Jin12Yingjie Zhao13Yingjie Zhao14Zhaofu Ma15Zhaofu Ma16Xiansheng Wu17Yang Yan18Wen Sun19Xiaohui Du20Guanglong Dong21Guanglong Dong22Medical School of Chinese PLA, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaMedical School of Chinese PLA, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaMedical School of Chinese PLA, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaMedical College of Qinghai University, Xining, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaMedical School of Chinese PLA, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaMedical School of Chinese PLA, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaMedical School of Chinese PLA, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaMedical School of Chinese PLA, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaDepartment of Anesthesiology, The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaMedical School of Chinese PLA, Beijing, ChinaDepartment of General Surgery, The First Medical Centre, Chinese PLA General Hospital, Beijing, ChinaBackground: Colorectal cancer (CRC) is the third most prevalent cancer worldwide and the second leading cause of cancer mortality. Signal transducer and activator of transcription (STAT) proteins are a group of transcription factors implicated in cell signal transduction and gene transcription in several cancer types. However, the level of expression, genetic alterations, and biological function of different STATs, as well as their prognostic and immunotherapeutic value in CRC remain unclear.Methods: The mRNA and protein expression levels, genetic alterations, prognostic value, gene–gene and protein–protein interaction networks, and biological function of STATs in CRC were studied using the GEPIA, HPA, cBioPortal, PrognoScan, Kaplan–Meier plotter, GeneMANIA, STRING, and Metascape databases. The expression of STATs in CRC was confirmed using immunohistochemistry (IHC). Finally, the relationship between STAT expression and immune infiltration as well as immunotherapy-associated indicators was also investigated.Results: The expression levels of STAT2/5A/5B are downregulated in CRC, and the STAT1/3/4/5B expressions were significantly associated with the tumor stage of patients with CRC. The abnormal expression of STAT2/4/5B in patients with CRC is related to the prognosis of patients with CRC. The STATs and their neighboring proteins are primarily associated with lymphocyte activation, cytokine-mediated signaling pathways, positive regulation of immune response, regulation of cytokine production, and growth hormone receptor signaling pathways in cancer. The expression of STATs was significantly associated with immune infiltration and immunotherapy response-associated indicators.Conclusion: This study may help further understand the molecular mechanism of CRC and provide new prognostic biomarkers and immunotherapy targets in patients with CRC.https://www.frontiersin.org/articles/10.3389/fgene.2022.951252/fullcolorectal cancerSTAT transcription factorsprognostic valueimmune infiltrationbioinformatics analysistumor immunotherapy |
| spellingShingle | Dingchang Li Dingchang Li Yanan Jiao Yanan Jiao Wenxing Gao Wenxing Gao Shidong Hu Dingling Li Wen Zhao Peng Chen Peng Chen Lujia Jin Lujia Jin Yingjie Zhao Yingjie Zhao Zhaofu Ma Zhaofu Ma Xiansheng Wu Yang Yan Wen Sun Xiaohui Du Guanglong Dong Guanglong Dong Comprehensive analysis of the prognostic and immunotherapeutic implications of STAT family members in human colorectal cancer Frontiers in Genetics colorectal cancer STAT transcription factors prognostic value immune infiltration bioinformatics analysis tumor immunotherapy |
| title | Comprehensive analysis of the prognostic and immunotherapeutic implications of STAT family members in human colorectal cancer |
| title_full | Comprehensive analysis of the prognostic and immunotherapeutic implications of STAT family members in human colorectal cancer |
| title_fullStr | Comprehensive analysis of the prognostic and immunotherapeutic implications of STAT family members in human colorectal cancer |
| title_full_unstemmed | Comprehensive analysis of the prognostic and immunotherapeutic implications of STAT family members in human colorectal cancer |
| title_short | Comprehensive analysis of the prognostic and immunotherapeutic implications of STAT family members in human colorectal cancer |
| title_sort | comprehensive analysis of the prognostic and immunotherapeutic implications of stat family members in human colorectal cancer |
| topic | colorectal cancer STAT transcription factors prognostic value immune infiltration bioinformatics analysis tumor immunotherapy |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.951252/full |
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