Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2
Background: The increase emergence and dissemination of antibiotic resistant Salmonella strains may contribute to a great risk on public health and impair classical treatment regime. The present study is aimed to investigate the prevalence of NTS in feces of broilers in four provinces in Iraq, and t...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, University of Kufa
2019-06-01
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| Series: | Kufa Journal for Veterinary Medical Sciences |
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| Online Access: | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/3331 |
| _version_ | 1827389118807539712 |
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| author | Esraa Razzaq Hassan Abdullah O. Alhatami |
| author_facet | Esraa Razzaq Hassan Abdullah O. Alhatami |
| author_sort | Esraa Razzaq Hassan |
| collection | DOAJ |
| description | Background: The increase emergence and dissemination of antibiotic resistant Salmonella strains may contribute to a great risk on public health and impair classical treatment regime. The present study is aimed to investigate the prevalence of NTS in feces of broilers in four provinces in Iraq, and the resistance profile of isolates to clinically important antibiotics.
Method: a total of 185 fecal samples were collected during the period from October2017 to March2018, from 28 broiler farms distributed in four governorates (Al- Najaf Al-Ashraf, AL-Qadyssia, Al-Muthana, and Babylon). Suspected Isolates of Salmonella were assayed for, detection of invA gene PCR, antibiotic susceptibility by Vitek-2.
Results: The prevalence of NTS infection in 28 examined poultry flocks was 75,7%. High prevalence rate of NTS was observed in Babylon (91,7%), followed by Al-Qadyssia (72,7%) and Al-Muthana (70%), which significantly differed (P ≤ 0.01) than reported in AL- Najaf (50%). While the isolation rate was 21.6%. All Salmonella isolates were positive for the invA gene. The majority of isolates were resistant to Minocycline (87.5%) and Ciprofloxacin (85%) with P value ≤ 0.01. in addition to these, 55% of strains were resistant to Pipracillin, 52,5% to Ticarcillin (p ≤ 0.05), and 37,5% to Gentamycin. Fortunately, all isolates were susceptible to Amikacin, Meropenem, Imipenem, Piperacillin/Tazobactam and Ticarcillin/Clavulanic acid, followed by 97.5% of strains were sensitive to Ceftazidime, Cefepime and Aztreanam. Twenty (50%) isolates were exhibited multiple resistance to three or more different antibacterial classes with different resistance profiles.
In conclusion, high prevalence of Salmonella and MDR Salmonella was found in broiler farms located in four Iraqi provinces. |
| first_indexed | 2024-03-08T16:29:52Z |
| format | Article |
| id | doaj.art-065d4bbe22b248f78fb2d415567b7499 |
| institution | Directory Open Access Journal |
| issn | 2077-9798 2959-8478 |
| language | English |
| last_indexed | 2024-03-08T16:29:52Z |
| publishDate | 2019-06-01 |
| publisher | Faculty of Veterinary Medicine, University of Kufa |
| record_format | Article |
| series | Kufa Journal for Veterinary Medical Sciences |
| spelling | doaj.art-065d4bbe22b248f78fb2d415567b74992024-01-06T10:11:46ZengFaculty of Veterinary Medicine, University of KufaKufa Journal for Veterinary Medical Sciences2077-97982959-84782019-06-0110110.36326/kjvs/2019/v10i13331Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2Esraa Razzaq Hassan0Abdullah O. Alhatami1Faculty of Veterinary Medicine, University of KufaFaculty of Veterinary Medicine, University of KufaBackground: The increase emergence and dissemination of antibiotic resistant Salmonella strains may contribute to a great risk on public health and impair classical treatment regime. The present study is aimed to investigate the prevalence of NTS in feces of broilers in four provinces in Iraq, and the resistance profile of isolates to clinically important antibiotics. Method: a total of 185 fecal samples were collected during the period from October2017 to March2018, from 28 broiler farms distributed in four governorates (Al- Najaf Al-Ashraf, AL-Qadyssia, Al-Muthana, and Babylon). Suspected Isolates of Salmonella were assayed for, detection of invA gene PCR, antibiotic susceptibility by Vitek-2. Results: The prevalence of NTS infection in 28 examined poultry flocks was 75,7%. High prevalence rate of NTS was observed in Babylon (91,7%), followed by Al-Qadyssia (72,7%) and Al-Muthana (70%), which significantly differed (P ≤ 0.01) than reported in AL- Najaf (50%). While the isolation rate was 21.6%. All Salmonella isolates were positive for the invA gene. The majority of isolates were resistant to Minocycline (87.5%) and Ciprofloxacin (85%) with P value ≤ 0.01. in addition to these, 55% of strains were resistant to Pipracillin, 52,5% to Ticarcillin (p ≤ 0.05), and 37,5% to Gentamycin. Fortunately, all isolates were susceptible to Amikacin, Meropenem, Imipenem, Piperacillin/Tazobactam and Ticarcillin/Clavulanic acid, followed by 97.5% of strains were sensitive to Ceftazidime, Cefepime and Aztreanam. Twenty (50%) isolates were exhibited multiple resistance to three or more different antibacterial classes with different resistance profiles. In conclusion, high prevalence of Salmonella and MDR Salmonella was found in broiler farms located in four Iraqi provinces.https://journal.uokufa.edu.iq/index.php/kjvs/article/view/3331SalmonellaMDRinv A genevitek-2 |
| spellingShingle | Esraa Razzaq Hassan Abdullah O. Alhatami Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2 Kufa Journal for Veterinary Medical Sciences Salmonella MDR inv A gene vitek-2 |
| title | Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2 |
| title_full | Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2 |
| title_fullStr | Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2 |
| title_full_unstemmed | Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2 |
| title_short | Antimicrobial susceptibility profile of Salmonella enterica isolated from poultry farms using Vitek- 2 |
| title_sort | antimicrobial susceptibility profile of salmonella enterica isolated from poultry farms using vitek 2 |
| topic | Salmonella MDR inv A gene vitek-2 |
| url | https://journal.uokufa.edu.iq/index.php/kjvs/article/view/3331 |
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