The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane
Abstract Caveolae are small coated plasma membrane invaginations with diverse functions. Caveolae undergo curvature changes. Yet, it is unclear which proteins regulate this process. To address this gap, we develop a correlative stimulated emission depletion (STED) fluorescence and platinum replica e...
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| Format: | Article |
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Nature Portfolio
2022-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-022-34958-3 |
| _version_ | 1827228328748122112 |
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| author | Claudia Matthaeus Kem A. Sochacki Andrea M. Dickey Dmytro Puchkov Volker Haucke Martin Lehmann Justin W. Taraska |
| author_facet | Claudia Matthaeus Kem A. Sochacki Andrea M. Dickey Dmytro Puchkov Volker Haucke Martin Lehmann Justin W. Taraska |
| author_sort | Claudia Matthaeus |
| collection | DOAJ |
| description | Abstract Caveolae are small coated plasma membrane invaginations with diverse functions. Caveolae undergo curvature changes. Yet, it is unclear which proteins regulate this process. To address this gap, we develop a correlative stimulated emission depletion (STED) fluorescence and platinum replica electron microscopy imaging (CLEM) method to image proteins at single caveolae. Caveolins and cavins are found at all caveolae, independent of curvature. EHD2 is detected at both low and highly curved caveolae. Pacsin2 associates with low curved caveolae and EHBP1 with mostly highly curved caveolae. Dynamin is absent from caveolae. Cells lacking dynamin show no substantial changes to caveolae, suggesting that dynamin is not directly involved in caveolae curvature. We propose a model where caveolins, cavins, and EHD2 assemble as a cohesive structural unit regulated by intermittent associations with pacsin2 and EHBP1. These coats can flatten and curve to enable lipid traffic, signaling, and changes to the surface area of the cell. |
| first_indexed | 2024-04-11T13:55:34Z |
| format | Article |
| id | doaj.art-065d783a60f54f6e8d7c9434225850b7 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2025-03-21T18:12:07Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-065d783a60f54f6e8d7c9434225850b72024-06-09T11:24:02ZengNature PortfolioNature Communications2041-17232022-11-0113111910.1038/s41467-022-34958-3The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membraneClaudia Matthaeus0Kem A. Sochacki1Andrea M. Dickey2Dmytro Puchkov3Volker Haucke4Martin Lehmann5Justin W. Taraska6Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of HealthBiochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of HealthBiochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of HealthLeibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP)Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of HealthAbstract Caveolae are small coated plasma membrane invaginations with diverse functions. Caveolae undergo curvature changes. Yet, it is unclear which proteins regulate this process. To address this gap, we develop a correlative stimulated emission depletion (STED) fluorescence and platinum replica electron microscopy imaging (CLEM) method to image proteins at single caveolae. Caveolins and cavins are found at all caveolae, independent of curvature. EHD2 is detected at both low and highly curved caveolae. Pacsin2 associates with low curved caveolae and EHBP1 with mostly highly curved caveolae. Dynamin is absent from caveolae. Cells lacking dynamin show no substantial changes to caveolae, suggesting that dynamin is not directly involved in caveolae curvature. We propose a model where caveolins, cavins, and EHD2 assemble as a cohesive structural unit regulated by intermittent associations with pacsin2 and EHBP1. These coats can flatten and curve to enable lipid traffic, signaling, and changes to the surface area of the cell.https://doi.org/10.1038/s41467-022-34958-3 |
| spellingShingle | Claudia Matthaeus Kem A. Sochacki Andrea M. Dickey Dmytro Puchkov Volker Haucke Martin Lehmann Justin W. Taraska The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane Nature Communications |
| title | The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane |
| title_full | The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane |
| title_fullStr | The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane |
| title_full_unstemmed | The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane |
| title_short | The molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane |
| title_sort | molecular organization of differentially curved caveolae indicates bendable structural units at the plasma membrane |
| url | https://doi.org/10.1038/s41467-022-34958-3 |
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