Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat
Abstract Background Previous studies have shown that seizures can cause cognitive disorders. On the other hand, the Curcuma zedoaria (CZ) has beneficial effects on the nervous system. However, there is little information on the possible effects of the CZ extract on seizures. The aim of this study wa...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-10-01
|
| Series: | Behavioral and Brain Functions |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12993-020-00169-3 |
| _version_ | 1819063532042321920 |
|---|---|
| author | Touran Mahmoudi Zahra Lorigooini Mahmoud Rafieian-kopaei Mehran Arabi Zahra Rabiei Elham Bijad Sedigheh Kazemi |
| author_facet | Touran Mahmoudi Zahra Lorigooini Mahmoud Rafieian-kopaei Mehran Arabi Zahra Rabiei Elham Bijad Sedigheh Kazemi |
| author_sort | Touran Mahmoudi |
| collection | DOAJ |
| description | Abstract Background Previous studies have shown that seizures can cause cognitive disorders. On the other hand, the Curcuma zedoaria (CZ) has beneficial effects on the nervous system. However, there is little information on the possible effects of the CZ extract on seizures. The aim of this study was to investigate the possible effects of CZ extract on cognitive impairment and oxidative stress induced by epilepsy in rats. Methods Rats were randomly divided into different groups. In all rats (except the sham group), kindling was performed by intraperitoneal injection of pentylenetetrazol (PTZ) at a dose of 35 mg/kg every 48 h for 14 days. Positive group received 2 mg/kg diazepam + PTZ; treatment groups received 100, 200 or 400 mg/kg CZ extract + PTZ; and one group received 0.5 mg/kg flumazenil and CZ extract + PTZ. Shuttle box and Morris Water Maze tests were used to measure memory and learning. On the last day of treatments PTZ injection was at dose of 60 mg/kg, tonic seizure threshold and mortality rate were recorded in each group. After deep anesthesia, blood was drawn from the rats’ hearts and the hippocampus of all rats was removed. Results Statistical analysis of the data showed that the CZ extract significantly increased the tonic seizure threshold and reduced the pentylenetetrazol-induced mortality and the extract dose of 400 mg/kg was selected as the most effective dose compared to the other doses. It was also found that flumazenil (a GABAA receptor antagonist) reduced the tonic seizure threshold compared to the effective dose of the extract. The results of shuttle box and Morris water maze behavioral tests showed that memory and learning decreased in the negative control group and the CZ extract treatment improved memory and learning in rats. The CZ extract also increased antioxidant capacity, decreased MDA and NO in the brain and serum of pre-treated groups in compared to the negative control group. Conclusion: It is concluded that the CZ extract has beneficial effects on learning and memory impairment in PTZ-induced epilepsy model, which has been associated with antioxidant effects in the brain or possibly exerts its effects through the GABAergic system. |
| first_indexed | 2024-12-21T15:16:10Z |
| format | Article |
| id | doaj.art-065db2c40697472385468791c976c107 |
| institution | Directory Open Access Journal |
| issn | 1744-9081 |
| language | English |
| last_indexed | 2024-12-21T15:16:10Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Behavioral and Brain Functions |
| spelling | doaj.art-065db2c40697472385468791c976c1072022-12-21T18:59:09ZengBMCBehavioral and Brain Functions1744-90812020-10-0116111210.1186/s12993-020-00169-3Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in ratTouran Mahmoudi0Zahra Lorigooini1Mahmoud Rafieian-kopaei2Mehran Arabi3Zahra Rabiei4Elham Bijad5Sedigheh Kazemi6Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical SciencesMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical SciencesMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical SciencesDepartment of Biology, Shahrekord UniversityMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical SciencesMedical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical SciencesCellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical SciencesAbstract Background Previous studies have shown that seizures can cause cognitive disorders. On the other hand, the Curcuma zedoaria (CZ) has beneficial effects on the nervous system. However, there is little information on the possible effects of the CZ extract on seizures. The aim of this study was to investigate the possible effects of CZ extract on cognitive impairment and oxidative stress induced by epilepsy in rats. Methods Rats were randomly divided into different groups. In all rats (except the sham group), kindling was performed by intraperitoneal injection of pentylenetetrazol (PTZ) at a dose of 35 mg/kg every 48 h for 14 days. Positive group received 2 mg/kg diazepam + PTZ; treatment groups received 100, 200 or 400 mg/kg CZ extract + PTZ; and one group received 0.5 mg/kg flumazenil and CZ extract + PTZ. Shuttle box and Morris Water Maze tests were used to measure memory and learning. On the last day of treatments PTZ injection was at dose of 60 mg/kg, tonic seizure threshold and mortality rate were recorded in each group. After deep anesthesia, blood was drawn from the rats’ hearts and the hippocampus of all rats was removed. Results Statistical analysis of the data showed that the CZ extract significantly increased the tonic seizure threshold and reduced the pentylenetetrazol-induced mortality and the extract dose of 400 mg/kg was selected as the most effective dose compared to the other doses. It was also found that flumazenil (a GABAA receptor antagonist) reduced the tonic seizure threshold compared to the effective dose of the extract. The results of shuttle box and Morris water maze behavioral tests showed that memory and learning decreased in the negative control group and the CZ extract treatment improved memory and learning in rats. The CZ extract also increased antioxidant capacity, decreased MDA and NO in the brain and serum of pre-treated groups in compared to the negative control group. Conclusion: It is concluded that the CZ extract has beneficial effects on learning and memory impairment in PTZ-induced epilepsy model, which has been associated with antioxidant effects in the brain or possibly exerts its effects through the GABAergic system.http://link.springer.com/article/10.1186/s12993-020-00169-3Curcuma zedoariaPentylenetetrazoleRepeated seizuresOxidative stressMemory |
| spellingShingle | Touran Mahmoudi Zahra Lorigooini Mahmoud Rafieian-kopaei Mehran Arabi Zahra Rabiei Elham Bijad Sedigheh Kazemi Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat Behavioral and Brain Functions Curcuma zedoaria Pentylenetetrazole Repeated seizures Oxidative stress Memory |
| title | Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat |
| title_full | Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat |
| title_fullStr | Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat |
| title_full_unstemmed | Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat |
| title_short | Effect of Curcuma zedoaria hydro-alcoholic extract on learning, memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat |
| title_sort | effect of curcuma zedoaria hydro alcoholic extract on learning memory deficits and oxidative damage of brain tissue following seizures induced by pentylenetetrazole in rat |
| topic | Curcuma zedoaria Pentylenetetrazole Repeated seizures Oxidative stress Memory |
| url | http://link.springer.com/article/10.1186/s12993-020-00169-3 |
| work_keys_str_mv | AT touranmahmoudi effectofcurcumazedoariahydroalcoholicextractonlearningmemorydeficitsandoxidativedamageofbraintissuefollowingseizuresinducedbypentylenetetrazoleinrat AT zahralorigooini effectofcurcumazedoariahydroalcoholicextractonlearningmemorydeficitsandoxidativedamageofbraintissuefollowingseizuresinducedbypentylenetetrazoleinrat AT mahmoudrafieiankopaei effectofcurcumazedoariahydroalcoholicextractonlearningmemorydeficitsandoxidativedamageofbraintissuefollowingseizuresinducedbypentylenetetrazoleinrat AT mehranarabi effectofcurcumazedoariahydroalcoholicextractonlearningmemorydeficitsandoxidativedamageofbraintissuefollowingseizuresinducedbypentylenetetrazoleinrat AT zahrarabiei effectofcurcumazedoariahydroalcoholicextractonlearningmemorydeficitsandoxidativedamageofbraintissuefollowingseizuresinducedbypentylenetetrazoleinrat AT elhambijad effectofcurcumazedoariahydroalcoholicextractonlearningmemorydeficitsandoxidativedamageofbraintissuefollowingseizuresinducedbypentylenetetrazoleinrat AT sedighehkazemi effectofcurcumazedoariahydroalcoholicextractonlearningmemorydeficitsandoxidativedamageofbraintissuefollowingseizuresinducedbypentylenetetrazoleinrat |