The Role of <i>Epinephelus coioides</i> DUSP5 in Regulating Singapore Grouper Iridovirus Infection
The dual-specificity phosphatase (DUSP) family plays an important role in response to adverse external factors. In this study, the DUSP5 from <i>Epinephelus coioides</i>, an important marine fish in Southeast Asia and China, was isolated and characterized. As expected, <i>E. coioid...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/9/1807 |
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| author | Jiayang He Yijie Cai Wei Huang Yunxiang Lin Yurong Lei Cuifen Huang Zongbin Cui Qiwei Qin Hongyan Sun |
| author_facet | Jiayang He Yijie Cai Wei Huang Yunxiang Lin Yurong Lei Cuifen Huang Zongbin Cui Qiwei Qin Hongyan Sun |
| author_sort | Jiayang He |
| collection | DOAJ |
| description | The dual-specificity phosphatase (DUSP) family plays an important role in response to adverse external factors. In this study, the DUSP5 from <i>Epinephelus coioides</i>, an important marine fish in Southeast Asia and China, was isolated and characterized. As expected, <i>E. coioides</i> DUSP5 contained four conserved domains: a rhodanese homology domain (RHOD); a dual-specificity phosphatase catalytic domain (DSPc); and two regions of low compositional complexity, indicating that <i>E. coioides</i> DUSP5 belongs to the DUSP family. <i>E. coioides</i> DUSP5 mRNA could be detected in all of the examined tissues, and was mainly distributed in the nucleus. Infection with Singapore grouper iridovirus (SGIV), one of the most important pathogens of marine fish, could inhibit the expression of <i>E. coioides</i> DUSP5. The overexpression of DUSP5 could significantly downregulate the expression of the key SGIV genes (MCP, ICP18, VP19, and LITAF), viral titers, the activity of NF-κB and AP-I, and the expression of pro-inflammatory factors (IL-6, IL-8, and TNF-α) of <i>E. coioides</i>, but could upregulate the expressions of caspase3 and p53, as well as SGIV-induced apoptosis. The results demonstrate that <i>E. coioides</i> DUSP5 could inhibit SGIV infection by regulating <i>E</i>. <i>coioides</i> immune-related factors, indicating that DUSP5 might be involved in viral infection. |
| first_indexed | 2024-03-10T21:52:07Z |
| format | Article |
| id | doaj.art-0661e22874a648f791f901dac954b886 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T21:52:07Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-0661e22874a648f791f901dac954b8862023-11-19T13:21:55ZengMDPI AGViruses1999-49152023-08-01159180710.3390/v15091807The Role of <i>Epinephelus coioides</i> DUSP5 in Regulating Singapore Grouper Iridovirus InfectionJiayang He0Yijie Cai1Wei Huang2Yunxiang Lin3Yurong Lei4Cuifen Huang5Zongbin Cui6Qiwei Qin7Hongyan Sun8State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, ChinaThe dual-specificity phosphatase (DUSP) family plays an important role in response to adverse external factors. In this study, the DUSP5 from <i>Epinephelus coioides</i>, an important marine fish in Southeast Asia and China, was isolated and characterized. As expected, <i>E. coioides</i> DUSP5 contained four conserved domains: a rhodanese homology domain (RHOD); a dual-specificity phosphatase catalytic domain (DSPc); and two regions of low compositional complexity, indicating that <i>E. coioides</i> DUSP5 belongs to the DUSP family. <i>E. coioides</i> DUSP5 mRNA could be detected in all of the examined tissues, and was mainly distributed in the nucleus. Infection with Singapore grouper iridovirus (SGIV), one of the most important pathogens of marine fish, could inhibit the expression of <i>E. coioides</i> DUSP5. The overexpression of DUSP5 could significantly downregulate the expression of the key SGIV genes (MCP, ICP18, VP19, and LITAF), viral titers, the activity of NF-κB and AP-I, and the expression of pro-inflammatory factors (IL-6, IL-8, and TNF-α) of <i>E. coioides</i>, but could upregulate the expressions of caspase3 and p53, as well as SGIV-induced apoptosis. The results demonstrate that <i>E. coioides</i> DUSP5 could inhibit SGIV infection by regulating <i>E</i>. <i>coioides</i> immune-related factors, indicating that DUSP5 might be involved in viral infection.https://www.mdpi.com/1999-4915/15/9/1807<i>Epinephelus coioides</i>DUSP5SGIVimmune responseapoptosis |
| spellingShingle | Jiayang He Yijie Cai Wei Huang Yunxiang Lin Yurong Lei Cuifen Huang Zongbin Cui Qiwei Qin Hongyan Sun The Role of <i>Epinephelus coioides</i> DUSP5 in Regulating Singapore Grouper Iridovirus Infection Viruses <i>Epinephelus coioides</i> DUSP5 SGIV immune response apoptosis |
| title | The Role of <i>Epinephelus coioides</i> DUSP5 in Regulating Singapore Grouper Iridovirus Infection |
| title_full | The Role of <i>Epinephelus coioides</i> DUSP5 in Regulating Singapore Grouper Iridovirus Infection |
| title_fullStr | The Role of <i>Epinephelus coioides</i> DUSP5 in Regulating Singapore Grouper Iridovirus Infection |
| title_full_unstemmed | The Role of <i>Epinephelus coioides</i> DUSP5 in Regulating Singapore Grouper Iridovirus Infection |
| title_short | The Role of <i>Epinephelus coioides</i> DUSP5 in Regulating Singapore Grouper Iridovirus Infection |
| title_sort | role of i epinephelus coioides i dusp5 in regulating singapore grouper iridovirus infection |
| topic | <i>Epinephelus coioides</i> DUSP5 SGIV immune response apoptosis |
| url | https://www.mdpi.com/1999-4915/15/9/1807 |
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