Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells
At a concentration of 4 g/L, an enteric polymer is utilized to target drug release in the small intestine and causes considerable toxicity in cells. Our ecology and ecosystem are also harmed by their non-biodegradable qualities. We isolated and identified polymer-degrading bacteria from industrial e...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2022-12-01
|
| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
| Subjects: | |
| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=24371 |
| _version_ | 1811188124711124992 |
|---|---|
| author | Shumona Akter Gobindo Kumar Paul Shafi Mahmud Md. Shamim Hossain Md. abu Abu Saleh Shahriar Zaman Md. Salah Uddin |
| author_facet | Shumona Akter Gobindo Kumar Paul Shafi Mahmud Md. Shamim Hossain Md. abu Abu Saleh Shahriar Zaman Md. Salah Uddin |
| author_sort | Shumona Akter |
| collection | DOAJ |
| description | At a concentration of 4 g/L, an enteric polymer is utilized to target drug release in the small intestine and causes considerable toxicity in cells. Our ecology and ecosystem are also harmed by their non-biodegradable qualities. We isolated and identified polymer-degrading bacteria from industrial effluent in this work. The isolated strain's morphological, biochemical, and antibiotic sensitivities were also examined. The isolated strain was found to be gram-negative, round-shaped, and non-motile in morphological tests, while biochemical tests revealed it to be negative in starch agar and TSI but positive in methyl red, mannitol salt, Simmon citrate, urea agar, and catalase test. The isolated strain was highly resistant to ciprofloxacin and vancomycin. The isolated bacterium was identified as Acinetobacter sp. by 16S rRNA sequencing. Additionally, Acinetobacter sp. strains of Escherichia coli and Brevibacillus sp. were used separately to observe the degradation of five synthesized non-biodegradable polymers (maleic acid propane-1,2 diol glycerol co-polyester, maleic acid phthalic acid propane-1,2 diol glycerol co-polyester, maleic acid phthalic acid butan-1,4 diol glycerol co-polyester, phthalic acid succinic acid propane-1,2 diol glycerol co-polyester, and phthalic acid succinic acid buten-1,4 diol glycerol co-polyester. The capacity of all three strains to degrade the above-mentioned polymers was greater than 75%. E. coli, for example, had a rapid disintegration rate but was responsible for human gastrointestinal and urinary tract infections. As a result, our isolated Acinetobacter sp. can be employed to degrade synthetic polymers. [ J Adv Biotechnol Exp Ther 2022; 5(3.000): 487-496] |
| first_indexed | 2024-04-11T14:15:17Z |
| format | Article |
| id | doaj.art-06661ae930274911a8f2f5f05b53f62c |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-04-11T14:15:17Z |
| publishDate | 2022-12-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-06661ae930274911a8f2f5f05b53f62c2022-12-22T04:19:33ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602022-12-015348749610.5455/jabet.2022.d13024371Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cellsShumona Akter0Gobindo Kumar Paul1Shafi Mahmud2Md. Shamim Hossain3Md. abu Abu Saleh4Shahriar Zaman5Md. Salah Uddin6Major in Social Infrastructure System Science, Ibaraki University, Ibaraki 316-8511, Japan Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh Division of Genome Sciences and Cancer, The John Curtin School of Medical Research, and The Shine-Dalgarno Centre for RNA Innovation, The Australian National University, Canberra, ACT 2601, Australia. Department of Biotechnology and Genetic Engineering, Islamic University, Kushtia-7003, Bangladesh. Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, Bangladesh Microbiology Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205, BangladeshAt a concentration of 4 g/L, an enteric polymer is utilized to target drug release in the small intestine and causes considerable toxicity in cells. Our ecology and ecosystem are also harmed by their non-biodegradable qualities. We isolated and identified polymer-degrading bacteria from industrial effluent in this work. The isolated strain's morphological, biochemical, and antibiotic sensitivities were also examined. The isolated strain was found to be gram-negative, round-shaped, and non-motile in morphological tests, while biochemical tests revealed it to be negative in starch agar and TSI but positive in methyl red, mannitol salt, Simmon citrate, urea agar, and catalase test. The isolated strain was highly resistant to ciprofloxacin and vancomycin. The isolated bacterium was identified as Acinetobacter sp. by 16S rRNA sequencing. Additionally, Acinetobacter sp. strains of Escherichia coli and Brevibacillus sp. were used separately to observe the degradation of five synthesized non-biodegradable polymers (maleic acid propane-1,2 diol glycerol co-polyester, maleic acid phthalic acid propane-1,2 diol glycerol co-polyester, maleic acid phthalic acid butan-1,4 diol glycerol co-polyester, phthalic acid succinic acid propane-1,2 diol glycerol co-polyester, and phthalic acid succinic acid buten-1,4 diol glycerol co-polyester. The capacity of all three strains to degrade the above-mentioned polymers was greater than 75%. E. coli, for example, had a rapid disintegration rate but was responsible for human gastrointestinal and urinary tract infections. As a result, our isolated Acinetobacter sp. can be employed to degrade synthetic polymers. [ J Adv Biotechnol Exp Ther 2022; 5(3.000): 487-496]http://www.ejmanager.com/fulltextpdf.php?mno=24371characterizationantibiotic sensitivitysynthetic polymersdegradation efficiency |
| spellingShingle | Shumona Akter Gobindo Kumar Paul Shafi Mahmud Md. Shamim Hossain Md. abu Abu Saleh Shahriar Zaman Md. Salah Uddin Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells Journal of Advanced Biotechnology and Experimental Therapeutics characterization antibiotic sensitivity synthetic polymers degradation efficiency |
| title | Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells |
| title_full | Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells |
| title_fullStr | Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells |
| title_full_unstemmed | Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells |
| title_short | Comparative polymer biodegradation efficiency of an isolated Acinetobacter sp. with Bravibacillus sp. and E. coli by resting cells |
| title_sort | comparative polymer biodegradation efficiency of an isolated acinetobacter sp with bravibacillus sp and e coli by resting cells |
| topic | characterization antibiotic sensitivity synthetic polymers degradation efficiency |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=24371 |
| work_keys_str_mv | AT shumonaakter comparativepolymerbiodegradationefficiencyofanisolatedacinetobacterspwithbravibacillusspandecolibyrestingcells AT gobindokumarpaul comparativepolymerbiodegradationefficiencyofanisolatedacinetobacterspwithbravibacillusspandecolibyrestingcells AT shafimahmud comparativepolymerbiodegradationefficiencyofanisolatedacinetobacterspwithbravibacillusspandecolibyrestingcells AT mdshamimhossain comparativepolymerbiodegradationefficiencyofanisolatedacinetobacterspwithbravibacillusspandecolibyrestingcells AT mdabuabusaleh comparativepolymerbiodegradationefficiencyofanisolatedacinetobacterspwithbravibacillusspandecolibyrestingcells AT shahriarzaman comparativepolymerbiodegradationefficiencyofanisolatedacinetobacterspwithbravibacillusspandecolibyrestingcells AT mdsalahuddin comparativepolymerbiodegradationefficiencyofanisolatedacinetobacterspwithbravibacillusspandecolibyrestingcells |