| Summary: | <i>Briareum stechei</i> is proven to be a rich source of 3,8-cyclized cembranoids (briarane) with a bicyclo[8.4.0] carbon core. In the present study, four previously unreported briaranes, briarenols W–Z (<b>1</b>–<b>4</b>), along with solenolide A (<b>5</b>), briarenolide M (<b>6</b>), briaexcavatolide F (<b>7</b>), and brianolide (<b>8</b>), were isolated and characterized through spectroscopic analysis, and the absolute configuration of <b>8</b> was corroborated by a single-crystal x-ray diffraction analysis. Briaranes <b>2</b> and <b>5</b> were found to induce significant inflammatory activity in lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophage cells by enhancing the expression of the inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) proteins.
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