Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samples
In this study, the second-generation high-throughput sequencing and DNA barcoding were combined to manually prepare multi-species mixed samples, and the mitochondrial gene CO І was used as a barcode to simultaneously identify the animal species in the mixed samples and identify endangered species. T...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2020-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/05/e3sconf_iaecst2020_01020.pdf |
| _version_ | 1818918302646272000 |
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| author | Yun-xia Chen Xiao-ming Xue Ya-lin Huang Yong-wu Zhou Sen-lin Hou Hai-tao Guo Jing Jiang |
| author_facet | Yun-xia Chen Xiao-ming Xue Ya-lin Huang Yong-wu Zhou Sen-lin Hou Hai-tao Guo Jing Jiang |
| author_sort | Yun-xia Chen |
| collection | DOAJ |
| description | In this study, the second-generation high-throughput sequencing and DNA barcoding were combined to manually prepare multi-species mixed samples, and the mitochondrial gene CO І was used as a barcode to simultaneously identify the animal species in the mixed samples and identify endangered species. The results showed that under the family and genus level, the simultaneous detection rate of the species in the mixed samples was as high as 100%, and the species identification rate was as high as 89% at the species level, and with high sensitivity, as little as 1% of the trace species could be detected. However, nearly 30% of non-target classification annotations appeared at the species level. It can be concluded that the mini CO I barcoding can be applied to the simultaneous identification of animal species in mixed biological samples, and the species identification rate is high. Non-target classification match existing at the species level can be further improved by increasing the length of the barcoding, improving the sequencing technology, reference database and so on. In this study, DNA metabarcoding technology was used to evaluate the feasibility of identification of endangered animals in multi-species mixed biological samples with CO І, in order to lay a preliminary foundation for the advancement of DNA metabarcoding method in the field of wildlife forensic identification. |
| first_indexed | 2024-12-20T00:47:48Z |
| format | Article |
| id | doaj.art-066cc770f44742dc8c6cb219d4d278d5 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-20T00:47:48Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-066cc770f44742dc8c6cb219d4d278d52022-12-21T19:59:21ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011450102010.1051/e3sconf/202014501020e3sconf_iaecst2020_01020Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samplesYun-xia ChenXiao-ming XueYa-lin HuangYong-wu ZhouSen-lin HouHai-tao GuoJing JiangIn this study, the second-generation high-throughput sequencing and DNA barcoding were combined to manually prepare multi-species mixed samples, and the mitochondrial gene CO І was used as a barcode to simultaneously identify the animal species in the mixed samples and identify endangered species. The results showed that under the family and genus level, the simultaneous detection rate of the species in the mixed samples was as high as 100%, and the species identification rate was as high as 89% at the species level, and with high sensitivity, as little as 1% of the trace species could be detected. However, nearly 30% of non-target classification annotations appeared at the species level. It can be concluded that the mini CO I barcoding can be applied to the simultaneous identification of animal species in mixed biological samples, and the species identification rate is high. Non-target classification match existing at the species level can be further improved by increasing the length of the barcoding, improving the sequencing technology, reference database and so on. In this study, DNA metabarcoding technology was used to evaluate the feasibility of identification of endangered animals in multi-species mixed biological samples with CO І, in order to lay a preliminary foundation for the advancement of DNA metabarcoding method in the field of wildlife forensic identification.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/05/e3sconf_iaecst2020_01020.pdf |
| spellingShingle | Yun-xia Chen Xiao-ming Xue Ya-lin Huang Yong-wu Zhou Sen-lin Hou Hai-tao Guo Jing Jiang Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samples E3S Web of Conferences |
| title | Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samples |
| title_full | Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samples |
| title_fullStr | Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samples |
| title_full_unstemmed | Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samples |
| title_short | Evaluation and application of mitochondrial CO І gene in identification of endangered wildlife in multi-species mixed biological samples |
| title_sort | evaluation and application of mitochondrial co і gene in identification of endangered wildlife in multi species mixed biological samples |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/05/e3sconf_iaecst2020_01020.pdf |
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