Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with <i>Paracoccidioides brasiliensis</i>: More Is Not Necessarily Better
Most people infected with the fungus <i>Paracoccidioides</i> spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used tw...
| Main Authors: | , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/6/4/311 |
| _version_ | 1827701590553788416 |
|---|---|
| author | Calliandra M. de-Souza-Silva Fabián Andrés Hurtado Aldo Henrique Tavares Getúlio P. de Oliveira Taina Raiol Christiane Nishibe Daniel Paiva Agustinho Nalvo Franco Almeida Maria Emília Machado Telles Walter André Moraes Nicola Anamélia Lorenzetti Bocca Patrícia Albuquerque Ildinete Silva-Pereira |
| author_facet | Calliandra M. de-Souza-Silva Fabián Andrés Hurtado Aldo Henrique Tavares Getúlio P. de Oliveira Taina Raiol Christiane Nishibe Daniel Paiva Agustinho Nalvo Franco Almeida Maria Emília Machado Telles Walter André Moraes Nicola Anamélia Lorenzetti Bocca Patrícia Albuquerque Ildinete Silva-Pereira |
| author_sort | Calliandra M. de-Souza-Silva |
| collection | DOAJ |
| description | Most people infected with the fungus <i>Paracoccidioides</i> spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used two mouse strains that are resistant (A/J) or susceptible (B10.A) to <i>P. brasiliensis</i> to study how dendritic cells (DCs) respond to the infection. RNA sequencing analysis showed that the susceptible strain DCs remodeled their transcriptomes much more intensely than those from the resistant strain, agreeing with a previous model of more intense innate immunity response in the susceptible strain. Contrastingly, these cells also repress genes/processes involved in antigen processing and presentation, such as lysosomal activity and autophagy. After the interaction with <i>P. brasiliensis</i>, both DCs and macrophages from the susceptible mouse reduced the autophagy marker LC3-II recruitment to the fungal phagosome compared to the resistant strain cells, confirming this pathway’s repression. These results suggest that impairment in antigen processing and presentation processes might be partially responsible for the inefficient activation of the adaptive immune response in this model. |
| first_indexed | 2024-03-10T14:37:31Z |
| format | Article |
| id | doaj.art-066d3a63c0c74c518e9c8d2512f7d9b0 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T14:37:31Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-066d3a63c0c74c518e9c8d2512f7d9b02023-11-20T22:04:00ZengMDPI AGJournal of Fungi2309-608X2020-11-016431110.3390/jof6040311Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with <i>Paracoccidioides brasiliensis</i>: More Is Not Necessarily BetterCalliandra M. de-Souza-Silva0Fabián Andrés Hurtado1Aldo Henrique Tavares2Getúlio P. de Oliveira3Taina Raiol4Christiane Nishibe5Daniel Paiva Agustinho6Nalvo Franco Almeida7Maria Emília Machado Telles Walter8André Moraes Nicola9Anamélia Lorenzetti Bocca10Patrícia Albuquerque11Ildinete Silva-Pereira12Laboratory of Molecular Biology of Pathogenic Fungi, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF 70910-900, BrazilLaboratory of Molecular Biology of Pathogenic Fungi, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF 70910-900, BrazilFaculty of Ceilândia, University of Brasília, Brasília, DF 72220-275, BrazilDivision of Allergy and Inflammation, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USAFiocruz Brasília, Oswaldo Cruz Foundation, Brasília, DF 70904-130, BrazilFaculty of Computing, Federal University of Mato Grosso do Sul, Campo Grande, MS 79070-900, BrazilDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, MO 63110-1093, USAFaculty of Computing, Federal University of Mato Grosso do Sul, Campo Grande, MS 79070-900, BrazilDepartment of Computer Science, University of Brasília, Brasília, DF 70910-900, BrazilFaculty of Medicine, University of Brasília, Brasília, DF 70910-900, BrazilLaboratory of Applied Immunology, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF 70910-900, BrazilLaboratory of Molecular Biology of Pathogenic Fungi, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF 70910-900, BrazilLaboratory of Molecular Biology of Pathogenic Fungi, Department of Cell Biology, Institute of Biological Sciences, University of Brasília, Brasília, DF 70910-900, BrazilMost people infected with the fungus <i>Paracoccidioides</i> spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used two mouse strains that are resistant (A/J) or susceptible (B10.A) to <i>P. brasiliensis</i> to study how dendritic cells (DCs) respond to the infection. RNA sequencing analysis showed that the susceptible strain DCs remodeled their transcriptomes much more intensely than those from the resistant strain, agreeing with a previous model of more intense innate immunity response in the susceptible strain. Contrastingly, these cells also repress genes/processes involved in antigen processing and presentation, such as lysosomal activity and autophagy. After the interaction with <i>P. brasiliensis</i>, both DCs and macrophages from the susceptible mouse reduced the autophagy marker LC3-II recruitment to the fungal phagosome compared to the resistant strain cells, confirming this pathway’s repression. These results suggest that impairment in antigen processing and presentation processes might be partially responsible for the inefficient activation of the adaptive immune response in this model.https://www.mdpi.com/2309-608X/6/4/311<i>Paracoccidioides brasiliensis</i>fungal innate immunitydendritic cellsresistancesusceptibilityA/J and B10.A mouse strains |
| spellingShingle | Calliandra M. de-Souza-Silva Fabián Andrés Hurtado Aldo Henrique Tavares Getúlio P. de Oliveira Taina Raiol Christiane Nishibe Daniel Paiva Agustinho Nalvo Franco Almeida Maria Emília Machado Telles Walter André Moraes Nicola Anamélia Lorenzetti Bocca Patrícia Albuquerque Ildinete Silva-Pereira Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with <i>Paracoccidioides brasiliensis</i>: More Is Not Necessarily Better Journal of Fungi <i>Paracoccidioides brasiliensis</i> fungal innate immunity dendritic cells resistance susceptibility A/J and B10.A mouse strains |
| title | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with <i>Paracoccidioides brasiliensis</i>: More Is Not Necessarily Better |
| title_full | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with <i>Paracoccidioides brasiliensis</i>: More Is Not Necessarily Better |
| title_fullStr | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with <i>Paracoccidioides brasiliensis</i>: More Is Not Necessarily Better |
| title_full_unstemmed | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with <i>Paracoccidioides brasiliensis</i>: More Is Not Necessarily Better |
| title_short | Transcriptional Remodeling Patterns in Murine Dendritic Cells Infected with <i>Paracoccidioides brasiliensis</i>: More Is Not Necessarily Better |
| title_sort | transcriptional remodeling patterns in murine dendritic cells infected with i paracoccidioides brasiliensis i more is not necessarily better |
| topic | <i>Paracoccidioides brasiliensis</i> fungal innate immunity dendritic cells resistance susceptibility A/J and B10.A mouse strains |
| url | https://www.mdpi.com/2309-608X/6/4/311 |
| work_keys_str_mv | AT calliandramdesouzasilva transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT fabianandreshurtado transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT aldohenriquetavares transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT getuliopdeoliveira transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT tainaraiol transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT christianenishibe transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT danielpaivaagustinho transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT nalvofrancoalmeida transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT mariaemiliamachadotelleswalter transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT andremoraesnicola transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT anamelialorenzettibocca transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT patriciaalbuquerque transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter AT ildinetesilvapereira transcriptionalremodelingpatternsinmurinedendriticcellsinfectedwithiparacoccidioidesbrasiliensisimoreisnotnecessarilybetter |