Efficient Transendothelial Migration of Latently HIV-1-Infected Cells
A small fraction of HIV-1-infected T cells forms populations of latently infected cells when they are a naive T-cell subset or in transit to a resting memory state. Latently HIV-1-infected cells reside in lymphoid tissues and serve as viral reservoirs. However, whether they systemically recirculate...
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MDPI AG
2021-08-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/8/1589 |
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| author | Reou Tanabe Yuko Morikawa |
| author_facet | Reou Tanabe Yuko Morikawa |
| author_sort | Reou Tanabe |
| collection | DOAJ |
| description | A small fraction of HIV-1-infected T cells forms populations of latently infected cells when they are a naive T-cell subset or in transit to a resting memory state. Latently HIV-1-infected cells reside in lymphoid tissues and serve as viral reservoirs. However, whether they systemically recirculate in the body and re-enter the lymphoid nodes are unknown. Here, we employed two in-vitro cell coculture systems mimicking the lymphatic endothelium in lymph nodes and investigated the homing potential, specifically the transendothelial migration (TEM), of two latently HIV-1-infected cell lines (J1.1 and ACH-2). In trans-well coculture systems, J1.1 and ACH-2 showed higher TEM efficiencies than their parental uninfected and acutely infected cells. The efficiency of TEM was enhanced by the presence of stromal cells, such as HS-5 and fibroblastic reticular cells. In an in-vitro reconstituted, three-dimensional coculture system in which stromal cells are embedded in collagen matrices, J1.1 showed slightly higher TEM efficiency in the presence of HS-5. In accordance with these phenotypes, latently infected cells adhered to the endothelial cells more efficiently than uninfected cells. Together, our study showed that latently HIV-1-infected cells enhanced cell adhesion and TEM abilities, suggesting their potential for efficient homing to lymph nodes. |
| first_indexed | 2024-03-10T08:17:12Z |
| format | Article |
| id | doaj.art-066e0bb1d8194f86975fdd1bf7e22505 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T08:17:12Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-066e0bb1d8194f86975fdd1bf7e225052023-11-22T10:11:44ZengMDPI AGViruses1999-49152021-08-01138158910.3390/v13081589Efficient Transendothelial Migration of Latently HIV-1-Infected CellsReou Tanabe0Yuko Morikawa1Graduate School of Infection Control Sciences, Kitasato University, Shirokane 5-9-1, Minato-ku, Tokyo 108-8641, JapanGraduate School of Infection Control Sciences, Kitasato University, Shirokane 5-9-1, Minato-ku, Tokyo 108-8641, JapanA small fraction of HIV-1-infected T cells forms populations of latently infected cells when they are a naive T-cell subset or in transit to a resting memory state. Latently HIV-1-infected cells reside in lymphoid tissues and serve as viral reservoirs. However, whether they systemically recirculate in the body and re-enter the lymphoid nodes are unknown. Here, we employed two in-vitro cell coculture systems mimicking the lymphatic endothelium in lymph nodes and investigated the homing potential, specifically the transendothelial migration (TEM), of two latently HIV-1-infected cell lines (J1.1 and ACH-2). In trans-well coculture systems, J1.1 and ACH-2 showed higher TEM efficiencies than their parental uninfected and acutely infected cells. The efficiency of TEM was enhanced by the presence of stromal cells, such as HS-5 and fibroblastic reticular cells. In an in-vitro reconstituted, three-dimensional coculture system in which stromal cells are embedded in collagen matrices, J1.1 showed slightly higher TEM efficiency in the presence of HS-5. In accordance with these phenotypes, latently infected cells adhered to the endothelial cells more efficiently than uninfected cells. Together, our study showed that latently HIV-1-infected cells enhanced cell adhesion and TEM abilities, suggesting their potential for efficient homing to lymph nodes.https://www.mdpi.com/1999-4915/13/8/1589transmigrationlymphocyte homingreconstitutionHIV-1latent infectionstromal cells |
| spellingShingle | Reou Tanabe Yuko Morikawa Efficient Transendothelial Migration of Latently HIV-1-Infected Cells Viruses transmigration lymphocyte homing reconstitution HIV-1 latent infection stromal cells |
| title | Efficient Transendothelial Migration of Latently HIV-1-Infected Cells |
| title_full | Efficient Transendothelial Migration of Latently HIV-1-Infected Cells |
| title_fullStr | Efficient Transendothelial Migration of Latently HIV-1-Infected Cells |
| title_full_unstemmed | Efficient Transendothelial Migration of Latently HIV-1-Infected Cells |
| title_short | Efficient Transendothelial Migration of Latently HIV-1-Infected Cells |
| title_sort | efficient transendothelial migration of latently hiv 1 infected cells |
| topic | transmigration lymphocyte homing reconstitution HIV-1 latent infection stromal cells |
| url | https://www.mdpi.com/1999-4915/13/8/1589 |
| work_keys_str_mv | AT reoutanabe efficienttransendothelialmigrationoflatentlyhiv1infectedcells AT yukomorikawa efficienttransendothelialmigrationoflatentlyhiv1infectedcells |