UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinoma
Abstract Background Ubiquitin-conjugating enzyme E2T (UBE2T) acts as an oncogene in various types of cancer. However, the mechanisms behind its oncogenic role remain unclear in lung cancer. This study aims to explore the function and clinical relevance of UBE2T in lung cancer. Methods Lentiviral vec...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-08-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-021-03056-1 |
| _version_ | 1818719182876835840 |
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| author | Jinhong Zhu Haijiao Ao Mingdong Liu Kui Cao Jianqun Ma |
| author_facet | Jinhong Zhu Haijiao Ao Mingdong Liu Kui Cao Jianqun Ma |
| author_sort | Jinhong Zhu |
| collection | DOAJ |
| description | Abstract Background Ubiquitin-conjugating enzyme E2T (UBE2T) acts as an oncogene in various types of cancer. However, the mechanisms behind its oncogenic role remain unclear in lung cancer. This study aims to explore the function and clinical relevance of UBE2T in lung cancer. Methods Lentiviral vectors were used to mediate UBE2T depletion or overexpress UBE2T in lung cancer cells. CCK8 analysis and western blotting were performed to investigate the effects of UBE2T on proliferation, autophagy, and relevant signaling pathways. To exploit the clinical significance of UBE2T, we performed immunohistochemistry staining with an anti-UBE2T antibody on 131 NSCLC samples. Moreover, we downloaded the human lung adenocarcinoma (LUAD) dataset from The Cancer Atlas Project (TCGA). Lasso Cox regression model was adopted to establish a prognostic model with UBE2T-correlated autophagy genes. Results We found that UBE2T stimulated proliferation and autophagy, and silencing this gene abolished autophagy in lung cancer cells. As suggested by Gene set enrichment analysis, we observed that UBE2T downregulated p53 levels in A549 cells and vice versa. Blockade of p53 counteracted the inhibitory effects of UBE2T depletion on autophagy. Meanwhile, the AMPK/mTOR signaling pathway was activated during UBE2T-mediated autophagy, suggesting that UBE2T promotes autophagy via the p53/AMPK/mTOR pathway. Interestingly, UBE2T overexpression increased cisplatin-trigged autophagy and led to cisplatin resistance of A549 cells, whereas inhibiting autophagy reversed drug resistance. However, no association was observed between UEB2T and overall survival in a population of 131 resectable NSCLC patients. Therefore, we developed and validated a multiple gene signature by considering UBE2T and its relevance in autophagy in lung cancer. The risk score derived from the prognostic signature significantly stratified LUAD patients into low- and high-risk groups with different overall survival. The risk score might independently predict prognosis. Interestingly, nomogram and decision curve analysis demonstrated that the signature’s prognostic accuracy culminated while combined with clinical features. Finally, the risk score showed great potential in predicting clinical chemosensitivity. Conclusions We found that UBE2T upregulates autophagy in NSCLC cells by activating the p53/AMPK/mTOR signaling pathway. The clinical predicting ability of UBE2T in LUAD can be improved by considering the autophagy-regulatory role of UBE2T. |
| first_indexed | 2024-12-17T20:02:53Z |
| format | Article |
| id | doaj.art-06784d37a31e47309d596e7b1efa21e0 |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-17T20:02:53Z |
| publishDate | 2021-08-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-06784d37a31e47309d596e7b1efa21e02022-12-21T21:34:24ZengBMCJournal of Translational Medicine1479-58762021-08-0119111710.1186/s12967-021-03056-1UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinomaJinhong Zhu0Haijiao Ao1Mingdong Liu2Kui Cao3Jianqun Ma4Department of Clinical Laboratory, Biobank, Harbin Medical University Cancer HospitalDepartment of Clinical Oncology, Harbin Medical University Cancer HospitalDepartment of Clinical Oncology, Harbin Medical University Cancer HospitalDepartment of Clinical Oncology, Harbin Medical University Cancer HospitalDepartment of Thoracic Surgery, Harbin Medical University Cancer HospitalAbstract Background Ubiquitin-conjugating enzyme E2T (UBE2T) acts as an oncogene in various types of cancer. However, the mechanisms behind its oncogenic role remain unclear in lung cancer. This study aims to explore the function and clinical relevance of UBE2T in lung cancer. Methods Lentiviral vectors were used to mediate UBE2T depletion or overexpress UBE2T in lung cancer cells. CCK8 analysis and western blotting were performed to investigate the effects of UBE2T on proliferation, autophagy, and relevant signaling pathways. To exploit the clinical significance of UBE2T, we performed immunohistochemistry staining with an anti-UBE2T antibody on 131 NSCLC samples. Moreover, we downloaded the human lung adenocarcinoma (LUAD) dataset from The Cancer Atlas Project (TCGA). Lasso Cox regression model was adopted to establish a prognostic model with UBE2T-correlated autophagy genes. Results We found that UBE2T stimulated proliferation and autophagy, and silencing this gene abolished autophagy in lung cancer cells. As suggested by Gene set enrichment analysis, we observed that UBE2T downregulated p53 levels in A549 cells and vice versa. Blockade of p53 counteracted the inhibitory effects of UBE2T depletion on autophagy. Meanwhile, the AMPK/mTOR signaling pathway was activated during UBE2T-mediated autophagy, suggesting that UBE2T promotes autophagy via the p53/AMPK/mTOR pathway. Interestingly, UBE2T overexpression increased cisplatin-trigged autophagy and led to cisplatin resistance of A549 cells, whereas inhibiting autophagy reversed drug resistance. However, no association was observed between UEB2T and overall survival in a population of 131 resectable NSCLC patients. Therefore, we developed and validated a multiple gene signature by considering UBE2T and its relevance in autophagy in lung cancer. The risk score derived from the prognostic signature significantly stratified LUAD patients into low- and high-risk groups with different overall survival. The risk score might independently predict prognosis. Interestingly, nomogram and decision curve analysis demonstrated that the signature’s prognostic accuracy culminated while combined with clinical features. Finally, the risk score showed great potential in predicting clinical chemosensitivity. Conclusions We found that UBE2T upregulates autophagy in NSCLC cells by activating the p53/AMPK/mTOR signaling pathway. The clinical predicting ability of UBE2T in LUAD can be improved by considering the autophagy-regulatory role of UBE2T.https://doi.org/10.1186/s12967-021-03056-1UBE2TLung cancerp53AutophagyPrognostic signature |
| spellingShingle | Jinhong Zhu Haijiao Ao Mingdong Liu Kui Cao Jianqun Ma UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinoma Journal of Translational Medicine UBE2T Lung cancer p53 Autophagy Prognostic signature |
| title | UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinoma |
| title_full | UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinoma |
| title_fullStr | UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinoma |
| title_full_unstemmed | UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinoma |
| title_short | UBE2T promotes autophagy via the p53/AMPK/mTOR signaling pathway in lung adenocarcinoma |
| title_sort | ube2t promotes autophagy via the p53 ampk mtor signaling pathway in lung adenocarcinoma |
| topic | UBE2T Lung cancer p53 Autophagy Prognostic signature |
| url | https://doi.org/10.1186/s12967-021-03056-1 |
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