Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi
Summary: Cell motility and biomechanics are critical in various (patho)physiological processes, including the regulation of vascular barrier integrity, which can be subverted by bacterial pathogens. Here, we present a protocol on how to expose endothelial cells (ECs) to vector-borne Borrelia burgdor...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007122 |
| _version_ | 1797980782932787200 |
|---|---|
| author | Marie Muenkel Raul Aparicio-Yuste Michal Caspi Tal Peter Kraiczy Effie E. Bastounis |
| author_facet | Marie Muenkel Raul Aparicio-Yuste Michal Caspi Tal Peter Kraiczy Effie E. Bastounis |
| author_sort | Marie Muenkel |
| collection | DOAJ |
| description | Summary: Cell motility and biomechanics are critical in various (patho)physiological processes, including the regulation of vascular barrier integrity, which can be subverted by bacterial pathogens. Here, we present a protocol on how to expose endothelial cells (ECs) to vector-borne Borrelia burgdorferi (Bb) and characterize EC kinematics and dynamics during exposure to live or heat-inactivated Bb through traction force and monolayer stress microscopy. Modifications to this protocol may be necessary for studying how different cell types interact with Bb or other microorganisms.For complete details on the use and execution of this protocol, please refer to Yuste et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-11T06:00:27Z |
| format | Article |
| id | doaj.art-067a329ecc0f4280ab6dc7139a81033b |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-11T06:00:27Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-067a329ecc0f4280ab6dc7139a81033b2022-12-22T04:41:43ZengElsevierSTAR Protocols2666-16672022-12-0134101832Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferiMarie Muenkel0Raul Aparicio-Yuste1Michal Caspi Tal2Peter Kraiczy3Effie E. Bastounis4Interfaculty Institute of Microbiology and Infection Medicine, Cluster of Excellence “Controlling Microbes to Fight Infections” (CMFI, EXC 2124), University of Tübingen, 72074 Tübingen, GermanyMultiscale in Mechanical and Biological Engineering (M2BE), Engineering Research Institute of Aragon (I3A), Department of Mechanical Engineering, University of Zaragoza, 50018 Zaragoza, SpainDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USAInstitute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Goethe University Frankfurt, 60438 Frankfurt am Main, GermanyInterfaculty Institute of Microbiology and Infection Medicine, Cluster of Excellence “Controlling Microbes to Fight Infections” (CMFI, EXC 2124), University of Tübingen, 72074 Tübingen, Germany; Eberhard Karls University of Tübingen, Auf der Morgenstelle 28/E8, 72076 Tübingen, Germany; Corresponding authorSummary: Cell motility and biomechanics are critical in various (patho)physiological processes, including the regulation of vascular barrier integrity, which can be subverted by bacterial pathogens. Here, we present a protocol on how to expose endothelial cells (ECs) to vector-borne Borrelia burgdorferi (Bb) and characterize EC kinematics and dynamics during exposure to live or heat-inactivated Bb through traction force and monolayer stress microscopy. Modifications to this protocol may be necessary for studying how different cell types interact with Bb or other microorganisms.For complete details on the use and execution of this protocol, please refer to Yuste et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166722007122BiophysicsCell BiologyCell cultureCell-based AssaysMicrobiologyMicroscopy |
| spellingShingle | Marie Muenkel Raul Aparicio-Yuste Michal Caspi Tal Peter Kraiczy Effie E. Bastounis Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi STAR Protocols Biophysics Cell Biology Cell culture Cell-based Assays Microbiology Microscopy |
| title | Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi |
| title_full | Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi |
| title_fullStr | Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi |
| title_full_unstemmed | Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi |
| title_short | Spatiotemporal characterization of endothelial cell motility and physical forces during exposure to Borrelia burgdorferi |
| title_sort | spatiotemporal characterization of endothelial cell motility and physical forces during exposure to borrelia burgdorferi |
| topic | Biophysics Cell Biology Cell culture Cell-based Assays Microbiology Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722007122 |
| work_keys_str_mv | AT mariemuenkel spatiotemporalcharacterizationofendothelialcellmotilityandphysicalforcesduringexposuretoborreliaburgdorferi AT raulaparicioyuste spatiotemporalcharacterizationofendothelialcellmotilityandphysicalforcesduringexposuretoborreliaburgdorferi AT michalcaspital spatiotemporalcharacterizationofendothelialcellmotilityandphysicalforcesduringexposuretoborreliaburgdorferi AT peterkraiczy spatiotemporalcharacterizationofendothelialcellmotilityandphysicalforcesduringexposuretoborreliaburgdorferi AT effieebastounis spatiotemporalcharacterizationofendothelialcellmotilityandphysicalforcesduringexposuretoborreliaburgdorferi |