Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities
Abstract Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for pe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-12-01
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| Series: | Microbiome |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40168-019-0769-4 |
| _version_ | 1818616571499642880 |
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| author | Ilia G. Halatchev David O’Donnell Matthew C. Hibberd Jeffrey I. Gordon |
| author_facet | Ilia G. Halatchev David O’Donnell Matthew C. Hibberd Jeffrey I. Gordon |
| author_sort | Ilia G. Halatchev |
| collection | DOAJ |
| description | Abstract Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for performing open-circuit indirect calorimetry on gnotobiotic mice colonized with gut microbial consortia obtained from different human donors. In this illustrative case, cultured collections of gut bacterial strains were obtained from obese and lean co-twins. The approach allows microbial contributions to host energy homeostasis to be characterized. |
| first_indexed | 2024-12-16T16:51:55Z |
| format | Article |
| id | doaj.art-067bede11b364e598ac65e5f3ee30b47 |
| institution | Directory Open Access Journal |
| issn | 2049-2618 |
| language | English |
| last_indexed | 2024-12-16T16:51:55Z |
| publishDate | 2019-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbiome |
| spelling | doaj.art-067bede11b364e598ac65e5f3ee30b472022-12-21T22:24:00ZengBMCMicrobiome2049-26182019-12-01711810.1186/s40168-019-0769-4Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communitiesIlia G. Halatchev0David O’Donnell1Matthew C. Hibberd2Jeffrey I. Gordon3The Edison Family Center for Genome Sciences and Systems BiologyThe Edison Family Center for Genome Sciences and Systems BiologyThe Edison Family Center for Genome Sciences and Systems BiologyThe Edison Family Center for Genome Sciences and Systems BiologyAbstract Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for performing open-circuit indirect calorimetry on gnotobiotic mice colonized with gut microbial consortia obtained from different human donors. In this illustrative case, cultured collections of gut bacterial strains were obtained from obese and lean co-twins. The approach allows microbial contributions to host energy homeostasis to be characterized.https://doi.org/10.1186/s40168-019-0769-4Gnotobiotic mouse husbandryHost phenotypingEnergy expenditureOpen-circuit indirect calorimetryBody compositionObesity |
| spellingShingle | Ilia G. Halatchev David O’Donnell Matthew C. Hibberd Jeffrey I. Gordon Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities Microbiome Gnotobiotic mouse husbandry Host phenotyping Energy expenditure Open-circuit indirect calorimetry Body composition Obesity |
| title | Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities |
| title_full | Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities |
| title_fullStr | Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities |
| title_full_unstemmed | Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities |
| title_short | Applying indirect open-circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities |
| title_sort | applying indirect open circuit calorimetry to study energy expenditure in gnotobiotic mice harboring different human gut microbial communities |
| topic | Gnotobiotic mouse husbandry Host phenotyping Energy expenditure Open-circuit indirect calorimetry Body composition Obesity |
| url | https://doi.org/10.1186/s40168-019-0769-4 |
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