Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams
The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a g...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2013-02-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100009&lng=en&tlng=en |
| _version_ | 1819069792707936256 |
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| author | L.F. Costa C.N. Nozaki N.S.C. Lira J.M.A.P. Antunes M.N. Xavier E.A. Costa T.A. Paixão R.L. Santos J. Megid |
| author_facet | L.F. Costa C.N. Nozaki N.S.C. Lira J.M.A.P. Antunes M.N. Xavier E.A. Costa T.A. Paixão R.L. Santos J. Megid |
| author_sort | L.F. Costa |
| collection | DOAJ |
| description | The aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 strain and samples of semen and urine were collected every week up to 180 days post infection. Out of 83 semen samples collected, 42 (50.6%) were positive for the species-specific nested PCR, and 23 (27.7%) were positive for the genus-specific PCR. Out of 75 urine samples, 49 (65.3%) were positive for the species-specific nested PCR, whereas 11 (14.6%) were genus-specific PCR positive. Species-specific nested PCR was significantly more sensitive (P<0.001) than the genus-specific PCR in semen and urine from experimentally infected rams. In conclusion, the species-specific nested PCR developed in this study may be used as a diagnostic tool for the detection of B. ovis in semen and urine samples from suspected rams. |
| first_indexed | 2024-12-21T16:55:41Z |
| format | Article |
| id | doaj.art-067d4a2c2dbe480eb29ca0fc38766104 |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-12-21T16:55:41Z |
| publishDate | 2013-02-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-067d4a2c2dbe480eb29ca0fc387661042022-12-21T18:56:45ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622013-02-01651556010.1590/S0102-09352013000100009S0102-09352013000100009Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in ramsL.F. Costa0C.N. Nozaki1N.S.C. Lira2J.M.A.P. Antunes3M.N. Xavier4E.A. Costa5T.A. Paixão6R.L. Santos7J. Megid8Universidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Estadual PaulistaUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Estadual PaulistaThe aim of the present study was to evaluate a species-specific nested PCR based on a previously described species-specific PCR for detection of B. ovis in semen and urine samples of experimentally infected rams. The performance of the species-specific nested PCR was compared with the results of a genus-specific PCR. Fourteen rams were experimentally infected with the Brucella ovis REO 198 strain and samples of semen and urine were collected every week up to 180 days post infection. Out of 83 semen samples collected, 42 (50.6%) were positive for the species-specific nested PCR, and 23 (27.7%) were positive for the genus-specific PCR. Out of 75 urine samples, 49 (65.3%) were positive for the species-specific nested PCR, whereas 11 (14.6%) were genus-specific PCR positive. Species-specific nested PCR was significantly more sensitive (P<0.001) than the genus-specific PCR in semen and urine from experimentally infected rams. In conclusion, the species-specific nested PCR developed in this study may be used as a diagnostic tool for the detection of B. ovis in semen and urine samples from suspected rams.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100009&lng=en&tlng=enBrucella ovisespécie-específicanested PCRsêmenurina |
| spellingShingle | L.F. Costa C.N. Nozaki N.S.C. Lira J.M.A.P. Antunes M.N. Xavier E.A. Costa T.A. Paixão R.L. Santos J. Megid Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams Arquivo Brasileiro de Medicina Veterinária e Zootecnia Brucella ovis espécie-específica nested PCR sêmen urina |
| title | Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams |
| title_full | Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams |
| title_fullStr | Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams |
| title_full_unstemmed | Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams |
| title_short | Species-specific nested PCR as a diagnostic tool for Brucella ovis infection in rams |
| title_sort | species specific nested pcr as a diagnostic tool for brucella ovis infection in rams |
| topic | Brucella ovis espécie-específica nested PCR sêmen urina |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000100009&lng=en&tlng=en |
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