USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME®
Cyclodextrins (CDs) are cyclic oligosaccharides, products of the intramolecular transglycosylation reaction promoted by the enzyme cyclomaltodextrin glucanotransferase (CGTase). The most commonly produced CDs are α-CD, β-CD and γ-CD, composed of 6, 7 and 8 glucose molecules, respectively. They form...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2024-03-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422024000300200&lng=pt&tlng=pt |
| _version_ | 1797265370855243776 |
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| author | Maria F. A. Aguiar Vanderson C. Fenelon Juliane H. Miyoshi Nathalia M. Valério Camila S. Mangolim Graciette Matioli |
| author_facet | Maria F. A. Aguiar Vanderson C. Fenelon Juliane H. Miyoshi Nathalia M. Valério Camila S. Mangolim Graciette Matioli |
| author_sort | Maria F. A. Aguiar |
| collection | DOAJ |
| description | Cyclodextrins (CDs) are cyclic oligosaccharides, products of the intramolecular transglycosylation reaction promoted by the enzyme cyclomaltodextrin glucanotransferase (CGTase). The most commonly produced CDs are α-CD, β-CD and γ-CD, composed of 6, 7 and 8 glucose molecules, respectively. They form non-covalent inclusion complexes with several molecules, increasing their stability and/or solubility. Due to the importance of CDs and the various industrial applications, this work aimed to use the commercial enzyme Toruzyme® to obtain CDs and direct its production to α-CD or β-CD through the use of ethanol and complexing agents in the reaction medium. The reaction medium added with ethanol and cyclohexanecarboxylic acid directed the production to β-CD, reaching 47.09 mg mL-1 of this molecule. The production medium containing ethanol and stearic acid proved to be more advantageous for the production of α-CD and, after 48 h of reaction, the production of α-CD reached 19.57 mg mL-1, a value six times greater than that of amount of β-CD produced. With this study it was possible to prove that with the use of ethanol and complexing agents in the reaction medium it is possible to direct the production of a certain CD and, thus, make different CGTases economically viable for industrial use. |
| first_indexed | 2024-04-25T00:43:43Z |
| format | Article |
| id | doaj.art-067f0d8f0dad48beaa47a4925eb202cb |
| institution | Directory Open Access Journal |
| issn | 1678-7064 |
| language | English |
| last_indexed | 2024-04-25T00:43:43Z |
| publishDate | 2024-03-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-067f0d8f0dad48beaa47a4925eb202cb2024-03-12T07:40:03ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642024-03-0147310.21577/0100-4042.20230105USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME®Maria F. A. AguiarVanderson C. FenelonJuliane H. MiyoshiNathalia M. ValérioCamila S. MangolimGraciette Matiolihttps://orcid.org/0000-0002-2531-2567Cyclodextrins (CDs) are cyclic oligosaccharides, products of the intramolecular transglycosylation reaction promoted by the enzyme cyclomaltodextrin glucanotransferase (CGTase). The most commonly produced CDs are α-CD, β-CD and γ-CD, composed of 6, 7 and 8 glucose molecules, respectively. They form non-covalent inclusion complexes with several molecules, increasing their stability and/or solubility. Due to the importance of CDs and the various industrial applications, this work aimed to use the commercial enzyme Toruzyme® to obtain CDs and direct its production to α-CD or β-CD through the use of ethanol and complexing agents in the reaction medium. The reaction medium added with ethanol and cyclohexanecarboxylic acid directed the production to β-CD, reaching 47.09 mg mL-1 of this molecule. The production medium containing ethanol and stearic acid proved to be more advantageous for the production of α-CD and, after 48 h of reaction, the production of α-CD reached 19.57 mg mL-1, a value six times greater than that of amount of β-CD produced. With this study it was possible to prove that with the use of ethanol and complexing agents in the reaction medium it is possible to direct the production of a certain CD and, thus, make different CGTases economically viable for industrial use.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422024000300200&lng=pt&tlng=ptcomplexing agentscyclohexanecarboxylic acidstearic acid. |
| spellingShingle | Maria F. A. Aguiar Vanderson C. Fenelon Juliane H. Miyoshi Nathalia M. Valério Camila S. Mangolim Graciette Matioli USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME® Química Nova complexing agents cyclohexanecarboxylic acid stearic acid. |
| title | USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME® |
| title_full | USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME® |
| title_fullStr | USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME® |
| title_full_unstemmed | USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME® |
| title_short | USO DE AGENTES COMPLEXANTES PARA O AUMENTO NA PRODUÇÃO DIRECIONADA DE CICLODEXTRINAS POR ENZIMA COMERCIAL TORUZYME® |
| title_sort | uso de agentes complexantes para o aumento na producao direcionada de ciclodextrinas por enzima comercial toruzyme r |
| topic | complexing agents cyclohexanecarboxylic acid stearic acid. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422024000300200&lng=pt&tlng=pt |
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