Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine
This work was designed to provide high protective, long-lasting immunity against FMD by enhancing the immunogenicity of the trivalent FMD vaccine using carbomer as adjuvant using G. pigs as an alternative cheapest animal model for quality control testing of the prepared FMD vaccines formulae. Guinea...
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| Format: | Article |
| Language: | English |
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Egyptian Society for Animal Management
2021-04-01
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| Series: | Journal of Applied Veterinary Sciences |
| Subjects: | |
| Online Access: | https://javs.journals.ekb.eg/article_154578.html |
| _version_ | 1830339488221495296 |
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| author | Walaa S. Shabana Abeer Abd EL-Sadek |
| author_facet | Walaa S. Shabana Abeer Abd EL-Sadek |
| author_sort | Walaa S. Shabana |
| collection | DOAJ |
| description | This work was designed to provide high protective, long-lasting immunity against FMD by enhancing the immunogenicity of the trivalent FMD vaccine using carbomer as adjuvant using G. pigs as an alternative cheapest animal model for quality control testing of the prepared FMD vaccines formulae. Guinea pigs were chosen as experimental models to develop concepts and techniques to study the PD50 of FMD vaccines because of the similarities of clinical symptoms in these animals to those of swine and cattle to saving cost, three different formulae of inactivated trivalent FMD vaccine including serotypes O Pan Asia2, A Iran O5 and SAT2/EGY/2012 were prepared as formula 1- (50% carbomer to 50% antigen); formula 2 (50% Montanide ISA 206 to 50%antigen) and formula 3- (25% Montanide ISA 206 and 25% carbomer with 50 % antigen). All of such formulae were found to be free from foreign contaminants, safe and potent, showing no postvaccinal reactions and high protective levels of specific FMD antibodies in Guinea pigs. Each vaccine formula's immunogenicity was determined by estimation of 50% Guinea pig protective dose (GPPD50) and monitoring of the humeral antibody response of vaccinated G. Pig groups. It was found that Montanide oils 206 with carbomer is the best vaccine formula, followed by Montanide oils 206 and finally carbomer which give early short-lasting immunity. |
| first_indexed | 2024-12-19T20:58:25Z |
| format | Article |
| id | doaj.art-06816c5d517b49dba6b6e22fcb1a8e82 |
| institution | Directory Open Access Journal |
| issn | 1687-4072 2090-3308 |
| language | English |
| last_indexed | 2024-12-19T20:58:25Z |
| publishDate | 2021-04-01 |
| publisher | Egyptian Society for Animal Management |
| record_format | Article |
| series | Journal of Applied Veterinary Sciences |
| spelling | doaj.art-06816c5d517b49dba6b6e22fcb1a8e822022-12-21T20:05:53ZengEgyptian Society for Animal ManagementJournal of Applied Veterinary Sciences1687-40722090-33082021-04-01622736https://dx.doi.org/10.21608/javs.2021.154578Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD VaccineWalaa S. Shabana0Abeer Abd EL-Sadek1Foot and Mouth Disease Research Department , Veterinary Serum and Vaccine Research Institute (VSVRI), Agricultural Research Center (ARC), Abassia, Cairo, Egypt.Department of Infectious Diseases, Faculty of Veterinary Medicine, El-Wadi El-Gadid University, Egypt.This work was designed to provide high protective, long-lasting immunity against FMD by enhancing the immunogenicity of the trivalent FMD vaccine using carbomer as adjuvant using G. pigs as an alternative cheapest animal model for quality control testing of the prepared FMD vaccines formulae. Guinea pigs were chosen as experimental models to develop concepts and techniques to study the PD50 of FMD vaccines because of the similarities of clinical symptoms in these animals to those of swine and cattle to saving cost, three different formulae of inactivated trivalent FMD vaccine including serotypes O Pan Asia2, A Iran O5 and SAT2/EGY/2012 were prepared as formula 1- (50% carbomer to 50% antigen); formula 2 (50% Montanide ISA 206 to 50%antigen) and formula 3- (25% Montanide ISA 206 and 25% carbomer with 50 % antigen). All of such formulae were found to be free from foreign contaminants, safe and potent, showing no postvaccinal reactions and high protective levels of specific FMD antibodies in Guinea pigs. Each vaccine formula's immunogenicity was determined by estimation of 50% Guinea pig protective dose (GPPD50) and monitoring of the humeral antibody response of vaccinated G. Pig groups. It was found that Montanide oils 206 with carbomer is the best vaccine formula, followed by Montanide oils 206 and finally carbomer which give early short-lasting immunity.https://javs.journals.ekb.eg/article_154578.htmladjuvantscarbomerfmdmontanide isa206 |
| spellingShingle | Walaa S. Shabana Abeer Abd EL-Sadek Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine Journal of Applied Veterinary Sciences adjuvants carbomer fmd montanide isa206 |
| title | Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine |
| title_full | Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine |
| title_fullStr | Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine |
| title_full_unstemmed | Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine |
| title_short | Effect of Carbomer as an Adjuvant for Enhancement of Immune-Response Against FMD Vaccine |
| title_sort | effect of carbomer as an adjuvant for enhancement of immune response against fmd vaccine |
| topic | adjuvants carbomer fmd montanide isa206 |
| url | https://javs.journals.ekb.eg/article_154578.html |
| work_keys_str_mv | AT walaasshabana effectofcarbomerasanadjuvantforenhancementofimmuneresponseagainstfmdvaccine AT abeerabdelsadek effectofcarbomerasanadjuvantforenhancementofimmuneresponseagainstfmdvaccine |