CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospects
The development of clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system has revolutionized genome editing and plant breeding. Applications of CRISPR/Cas9 technology in fruit crops, including grapevine, enable precise improvement of agronomically...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Maximum Academic Press
2022-01-01
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| Series: | Fruit Research |
| Subjects: | |
| Online Access: | https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0007 |
| _version_ | 1797293139442008064 |
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| author | Chong Ren Yanping Lin Zhenchang Liang |
| author_facet | Chong Ren Yanping Lin Zhenchang Liang |
| author_sort | Chong Ren |
| collection | DOAJ |
| description | The development of clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system has revolutionized genome editing and plant breeding. Applications of CRISPR/Cas9 technology in fruit crops, including grapevine, enable precise improvement of agronomically important traits. In this review, we first describe genome editing based on the most widely used CRISPR/Cas9 system and recently developed CRISPR technologies. We then focus on applications of CRISPR/Cas9 in improvement of disease resistance, optimization of CRISPR/Cas9 systems, multiplex genome editing and off-target effect analysis in grapevine. We also discuss the challenges facing genome editing that should be overcome to realize the potential of CRISPR technology in grapevine. Finally, we highlight possible future experimental considerations for more precise and efficient genome editing in grapevine. |
| first_indexed | 2024-03-07T20:08:33Z |
| format | Article |
| id | doaj.art-0685bb04619d4372b421a27bbd8e4614 |
| institution | Directory Open Access Journal |
| issn | 2769-4615 |
| language | English |
| last_indexed | 2024-03-07T20:08:33Z |
| publishDate | 2022-01-01 |
| publisher | Maximum Academic Press |
| record_format | Article |
| series | Fruit Research |
| spelling | doaj.art-0685bb04619d4372b421a27bbd8e46142024-02-28T03:19:34ZengMaximum Academic PressFruit Research2769-46152022-01-01211910.48130/FruRes-2022-0007FruRes-2022-0007CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospectsChong Ren0Yanping Lin1Zhenchang Liang2Beijing Key Laboratory of Grape Sciences and Enology, Key Laboratory of Plant Resource, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR ChinaBeijing Key Laboratory of Grape Sciences and Enology, Key Laboratory of Plant Resource, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR ChinaBeijing Key Laboratory of Grape Sciences and Enology, Key Laboratory of Plant Resource, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, PR ChinaThe development of clustered regularly interspaced short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) system has revolutionized genome editing and plant breeding. Applications of CRISPR/Cas9 technology in fruit crops, including grapevine, enable precise improvement of agronomically important traits. In this review, we first describe genome editing based on the most widely used CRISPR/Cas9 system and recently developed CRISPR technologies. We then focus on applications of CRISPR/Cas9 in improvement of disease resistance, optimization of CRISPR/Cas9 systems, multiplex genome editing and off-target effect analysis in grapevine. We also discuss the challenges facing genome editing that should be overcome to realize the potential of CRISPR technology in grapevine. Finally, we highlight possible future experimental considerations for more precise and efficient genome editing in grapevine.https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0007crispr/cas9genome editinggrapevineadvance |
| spellingShingle | Chong Ren Yanping Lin Zhenchang Liang CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospects Fruit Research crispr/cas9 genome editing grapevine advance |
| title | CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospects |
| title_full | CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospects |
| title_fullStr | CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospects |
| title_full_unstemmed | CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospects |
| title_short | CRISPR/Cas genome editing in grapevine: recent advances, challenges and future prospects |
| title_sort | crispr cas genome editing in grapevine recent advances challenges and future prospects |
| topic | crispr/cas9 genome editing grapevine advance |
| url | https://www.maxapress.com/article/doi/10.48130/FruRes-2022-0007 |
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