The impact of autophagy on arbovirus infection of mosquito cells.
Macroautophagy is an evolutionarily conserved cellular process critical for maintaining cellular homeostasis. It can additionally function as an innate immune response to viral infection as has been demonstrated for a number of arthropod-borne (arbo-) viruses. Arboviruses are maintained in a transmi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-05-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://doi.org/10.1371/journal.pntd.0007754 |
| _version_ | 1818726279693729792 |
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| author | Doug E Brackney Maria A Correa Duncan W Cozens |
| author_facet | Doug E Brackney Maria A Correa Duncan W Cozens |
| author_sort | Doug E Brackney |
| collection | DOAJ |
| description | Macroautophagy is an evolutionarily conserved cellular process critical for maintaining cellular homeostasis. It can additionally function as an innate immune response to viral infection as has been demonstrated for a number of arthropod-borne (arbo-) viruses. Arboviruses are maintained in a transmission cycle between vertebrate hosts and invertebrate vectors yet the majority of studies assessing autophagy-arbovirus interactions have been limited to the mammalian host. Therefore we evaluated the role of autophagy during arbovirus infection of the invertebrate vector using the tractable Aag2 Aedes aegypti mosquito cell culture system. Our data demonstrates that autophagy is significantly induced in mosquito cells upon infection with two divergent arboviruses: dengue virus-2 (DENV-2; Flaviviridae, Flavivirus) and chikungunya virus (CHIKV; Togaviridae, Alphavirus). While assessing the role of autophagy during arbovirus infection, we observed a somewhat paradoxical outcome. Both induction and suppression of autophagy via torin-1 and spautin-1, respectively, resulted in increased viral titers for both viruses, yet suppression of autophagy-related genes had no effect. Interestingly, chemical modulators of autophagy had either no effect or opposite effects in another widely used mosquito cell line, C6/36 Aedes albopictus cells. Together, our data reveals a limited role for autophagy during arbovirus infection of mosquito cells. Further, our findings suggest that commonly used chemical modulators of autophagy alter mosquito cells in such a way as to promote viral replication; however, it is unclear if this occurs directly through autophagic manipulation or other means. |
| first_indexed | 2024-12-17T21:55:41Z |
| format | Article |
| id | doaj.art-06868a12cb3d406eb40d5b9b3ce03667 |
| institution | Directory Open Access Journal |
| issn | 1935-2727 1935-2735 |
| language | English |
| last_indexed | 2024-12-17T21:55:41Z |
| publishDate | 2020-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj.art-06868a12cb3d406eb40d5b9b3ce036672022-12-21T21:31:07ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352020-05-01145e000775410.1371/journal.pntd.0007754The impact of autophagy on arbovirus infection of mosquito cells.Doug E BrackneyMaria A CorreaDuncan W CozensMacroautophagy is an evolutionarily conserved cellular process critical for maintaining cellular homeostasis. It can additionally function as an innate immune response to viral infection as has been demonstrated for a number of arthropod-borne (arbo-) viruses. Arboviruses are maintained in a transmission cycle between vertebrate hosts and invertebrate vectors yet the majority of studies assessing autophagy-arbovirus interactions have been limited to the mammalian host. Therefore we evaluated the role of autophagy during arbovirus infection of the invertebrate vector using the tractable Aag2 Aedes aegypti mosquito cell culture system. Our data demonstrates that autophagy is significantly induced in mosquito cells upon infection with two divergent arboviruses: dengue virus-2 (DENV-2; Flaviviridae, Flavivirus) and chikungunya virus (CHIKV; Togaviridae, Alphavirus). While assessing the role of autophagy during arbovirus infection, we observed a somewhat paradoxical outcome. Both induction and suppression of autophagy via torin-1 and spautin-1, respectively, resulted in increased viral titers for both viruses, yet suppression of autophagy-related genes had no effect. Interestingly, chemical modulators of autophagy had either no effect or opposite effects in another widely used mosquito cell line, C6/36 Aedes albopictus cells. Together, our data reveals a limited role for autophagy during arbovirus infection of mosquito cells. Further, our findings suggest that commonly used chemical modulators of autophagy alter mosquito cells in such a way as to promote viral replication; however, it is unclear if this occurs directly through autophagic manipulation or other means.https://doi.org/10.1371/journal.pntd.0007754 |
| spellingShingle | Doug E Brackney Maria A Correa Duncan W Cozens The impact of autophagy on arbovirus infection of mosquito cells. PLoS Neglected Tropical Diseases |
| title | The impact of autophagy on arbovirus infection of mosquito cells. |
| title_full | The impact of autophagy on arbovirus infection of mosquito cells. |
| title_fullStr | The impact of autophagy on arbovirus infection of mosquito cells. |
| title_full_unstemmed | The impact of autophagy on arbovirus infection of mosquito cells. |
| title_short | The impact of autophagy on arbovirus infection of mosquito cells. |
| title_sort | impact of autophagy on arbovirus infection of mosquito cells |
| url | https://doi.org/10.1371/journal.pntd.0007754 |
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