Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022
Background and Aim: Cockroaches are potential carriers of a wide range of pathogenic microorganisms (fungi, bacteria and parasites). Considering the importance of these insects in the transmission of pathogenic fungi and their impact on the health of hospitalized people, the aim of this study was is...
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Tehran University of Medical Sciences
2024-12-01
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| Series: | پیاورد سلامت |
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| Online Access: | http://payavard.tums.ac.ir/article-1-7561-en.pdf |
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| author | Hanie Pooshideh Jalal Jafarzade Ali Heidarpour Mojtaba Taghizade Armaki saeid Mahdavi Omran Firoozeh Kermani Mohsen Karami |
| author_facet | Hanie Pooshideh Jalal Jafarzade Ali Heidarpour Mojtaba Taghizade Armaki saeid Mahdavi Omran Firoozeh Kermani Mohsen Karami |
| author_sort | Hanie Pooshideh |
| collection | DOAJ |
| description | Background and Aim: Cockroaches are potential carriers of a wide range of pathogenic microorganisms (fungi, bacteria and parasites). Considering the importance of these insects in the transmission of pathogenic fungi and their impact on the health of hospitalized people, the aim of this study was isolation and molecular identification of pathogenic fungi from the external and internal surfaces of cockroaches collected from three educational hospitals of Babol city, Mazandaran Province, Iran.
Materials and Methods: Cockroaches were caught by a sterile manual method and after washing to remove surface contamination with 70% ethyl alcohol for 2min, 100 µL of the solutions from the external and internal parts of cockroaches were cultured on Sabouraud’s dextrose agar with 0.05% chloramphenicol and incubated at 30 °C for 3 weeks. The different yeast and filamentous colonies were distinguished using standard mycological methods. The PCR-RFLP method and the restriction enzymes Msp I and Bln I were used for the differentiation of Candida species, and the restriction enzyme Alw I for Aspergillus species.
Results: A total of 85 fungi species/genera were isolated from all 50 studied cockroaches, including German cockroaches 15 (30%), American cockroach 20 (40%) and brown 15 (30%), which included 52 isolates from external surfaces (61%) and 33 isolates from internal surfaces of cockroaches (39%). Of the total number of 49 filamentous fungi, Aspergillus fumigatus 16 (32.65%) and out of the total number of 36 yeasts identified, Candida albicans 14 (38.89%) were the most common fungi isolated in this study. Other filamentous fungi and yeasts isolated from cockroaches include Aspergillus niger 11 (22.45%), Aspergillus flavus 8 (16.32%), Penicillium 4 (8.2%), Rhizopus 3 (6.1%), Cladosporium 3 (6.1%), Mucor 2 (4%), Alternaria 2 (4%), Candida parapsilosis 6 (16.67%), Candida krusei 12 (33.33%), Candida glabrata 3 (8.33%), and Candida tropicalis 1 (2.78%) were isolated.
Conclusion: Considering the isolation of several fungal species from cockroaches and their importance in the possible mechanical transmission of common fungal infections in hospitals, these insects can be a source of continuous transmission of infection, if there is no regular preventive disinfection of the hospital environment. |
| first_indexed | 2025-02-17T07:03:13Z |
| format | Article |
| id | doaj.art-068820d4acbe445ba52cffb62faab750 |
| institution | Directory Open Access Journal |
| issn | 1735-8132 2008-2665 |
| language | fas |
| last_indexed | 2025-02-17T07:03:13Z |
| publishDate | 2024-12-01 |
| publisher | Tehran University of Medical Sciences |
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| series | پیاورد سلامت |
| spelling | doaj.art-068820d4acbe445ba52cffb62faab7502025-01-06T08:46:56ZfasTehran University of Medical Sciencesپیاورد سلامت1735-81322008-26652024-12-0118294105Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022Hanie Pooshideh0Jalal Jafarzade1Ali Heidarpour2Mojtaba Taghizade Armaki3saeid Mahdavi Omran4Firoozeh Kermani5Mohsen Karami6 General Medicine Student, School of Medicine, Babol University of Medical Sciences, Babol, Iran Master of Science in Medical Mycology, School of Medicine, Babol University of Medical Sciences, Babol, Iran Master of Science in Entomology, School of Public Health, Babol University of Medical Sciences, Babol, Iran Associate Professor, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Professor, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Assistant Professor, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Associate Professor, Infectious Diseases and Tropical Medicine Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Background and Aim: Cockroaches are potential carriers of a wide range of pathogenic microorganisms (fungi, bacteria and parasites). Considering the importance of these insects in the transmission of pathogenic fungi and their impact on the health of hospitalized people, the aim of this study was isolation and molecular identification of pathogenic fungi from the external and internal surfaces of cockroaches collected from three educational hospitals of Babol city, Mazandaran Province, Iran. Materials and Methods: Cockroaches were caught by a sterile manual method and after washing to remove surface contamination with 70% ethyl alcohol for 2min, 100 µL of the solutions from the external and internal parts of cockroaches were cultured on Sabouraud’s dextrose agar with 0.05% chloramphenicol and incubated at 30 °C for 3 weeks. The different yeast and filamentous colonies were distinguished using standard mycological methods. The PCR-RFLP method and the restriction enzymes Msp I and Bln I were used for the differentiation of Candida species, and the restriction enzyme Alw I for Aspergillus species. Results: A total of 85 fungi species/genera were isolated from all 50 studied cockroaches, including German cockroaches 15 (30%), American cockroach 20 (40%) and brown 15 (30%), which included 52 isolates from external surfaces (61%) and 33 isolates from internal surfaces of cockroaches (39%). Of the total number of 49 filamentous fungi, Aspergillus fumigatus 16 (32.65%) and out of the total number of 36 yeasts identified, Candida albicans 14 (38.89%) were the most common fungi isolated in this study. Other filamentous fungi and yeasts isolated from cockroaches include Aspergillus niger 11 (22.45%), Aspergillus flavus 8 (16.32%), Penicillium 4 (8.2%), Rhizopus 3 (6.1%), Cladosporium 3 (6.1%), Mucor 2 (4%), Alternaria 2 (4%), Candida parapsilosis 6 (16.67%), Candida krusei 12 (33.33%), Candida glabrata 3 (8.33%), and Candida tropicalis 1 (2.78%) were isolated. Conclusion: Considering the isolation of several fungal species from cockroaches and their importance in the possible mechanical transmission of common fungal infections in hospitals, these insects can be a source of continuous transmission of infection, if there is no regular preventive disinfection of the hospital environment.http://payavard.tums.ac.ir/article-1-7561-en.pdfcockroachbabol teaching hospitalspathogenic fungi |
| spellingShingle | Hanie Pooshideh Jalal Jafarzade Ali Heidarpour Mojtaba Taghizade Armaki saeid Mahdavi Omran Firoozeh Kermani Mohsen Karami Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022 پیاورد سلامت cockroach babol teaching hospitals pathogenic fungi |
| title | Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022 |
| title_full | Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022 |
| title_fullStr | Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022 |
| title_full_unstemmed | Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022 |
| title_short | Isolation and Molecular Identification of Pathogenic Fungi from Cockroaches Caught from Babol Teaching Hospitals during 2021-2022 |
| title_sort | isolation and molecular identification of pathogenic fungi from cockroaches caught from babol teaching hospitals during 2021 2022 |
| topic | cockroach babol teaching hospitals pathogenic fungi |
| url | http://payavard.tums.ac.ir/article-1-7561-en.pdf |
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