Transcriptome analysis of Auricularia fibrillifera fruit-body responses to drought stress and rehydration
Abstract Background Drought stress severely restricts edible fungus production. The genus Auricularia has a rare drought tolerance, a rehydration capability, and is nutrient rich. Results The key genes and metabolic pathways involved in drought-stress and rehydration were investigated using a transc...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-021-08284-9 |
| _version_ | 1831715550201380864 |
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| author | Yiqin Wang Zhifen Yang Luxi Shi Rui Yang Hao Guo Suqin Zhang Guangdong Geng |
| author_facet | Yiqin Wang Zhifen Yang Luxi Shi Rui Yang Hao Guo Suqin Zhang Guangdong Geng |
| author_sort | Yiqin Wang |
| collection | DOAJ |
| description | Abstract Background Drought stress severely restricts edible fungus production. The genus Auricularia has a rare drought tolerance, a rehydration capability, and is nutrient rich. Results The key genes and metabolic pathways involved in drought-stress and rehydration were investigated using a transcriptome analysis to clarify the relevant molecular mechanisms. In total, 173.93 Mb clean reads, 26.09 Gb of data bulk, and 52,954 unigenes were obtained. Under drought-stress and rehydration conditions, 14,235 and 8539 differentially expressed genes, respectively, were detected. ‘Tyrosine metabolic’, ‘caffeine metabolism’, ‘ribosome’, ‘phagosome’, and ‘proline and arginine metabolism’, as well as ‘peroxisome’ and ‘mitogen-activated protein kinase signaling’ pathways, had major roles in A. fibrillifera responses to drought stress. ‘Tyrosine’ and ‘caffeine metabolism’ might reveal unknown mechanisms for the antioxidation of A. fibrillifera under drought-stress conditions. During the rehydration process, ‘diterpenoid biosynthesis’, ‘butanoate metabolism’, ‘C5-branched dibasic acid’, and ‘aflatoxin biosynthesis’ pathways were significantly enriched. Gibberellins and γ-aminobutyric acid were important in the recovery of A. fibrillifera growth after rehydration. Many genes related to antibiotics, vitamins, and other health-related ingredients were found in A. fibrillifera. Conclusion These findings suggested that the candidate genes and metabolites involved in crucial biological pathways might regulate the drought tolerance or rehydration of Auricularia, shedding light on the corresponding mechanisms and providing new potential targets for the breeding and cultivation of drought-tolerant fungi. |
| first_indexed | 2024-12-21T00:21:10Z |
| format | Article |
| id | doaj.art-06890039049c4f708e4c4950bc0cdde6 |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-21T00:21:10Z |
| publishDate | 2022-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-06890039049c4f708e4c4950bc0cdde62022-12-21T19:22:06ZengBMCBMC Genomics1471-21642022-01-0123111710.1186/s12864-021-08284-9Transcriptome analysis of Auricularia fibrillifera fruit-body responses to drought stress and rehydrationYiqin Wang0Zhifen Yang1Luxi Shi2Rui Yang3Hao Guo4Suqin Zhang5Guangdong Geng6College of Agriculture, Guizhou UniversityCollege of Agriculture, Guizhou UniversityCollege of Agriculture, Guizhou UniversityCollege of Agriculture, Guizhou UniversityCollege of Agriculture, Guizhou UniversityCollege of Agriculture, Guizhou UniversityCollege of Agriculture, Guizhou UniversityAbstract Background Drought stress severely restricts edible fungus production. The genus Auricularia has a rare drought tolerance, a rehydration capability, and is nutrient rich. Results The key genes and metabolic pathways involved in drought-stress and rehydration were investigated using a transcriptome analysis to clarify the relevant molecular mechanisms. In total, 173.93 Mb clean reads, 26.09 Gb of data bulk, and 52,954 unigenes were obtained. Under drought-stress and rehydration conditions, 14,235 and 8539 differentially expressed genes, respectively, were detected. ‘Tyrosine metabolic’, ‘caffeine metabolism’, ‘ribosome’, ‘phagosome’, and ‘proline and arginine metabolism’, as well as ‘peroxisome’ and ‘mitogen-activated protein kinase signaling’ pathways, had major roles in A. fibrillifera responses to drought stress. ‘Tyrosine’ and ‘caffeine metabolism’ might reveal unknown mechanisms for the antioxidation of A. fibrillifera under drought-stress conditions. During the rehydration process, ‘diterpenoid biosynthesis’, ‘butanoate metabolism’, ‘C5-branched dibasic acid’, and ‘aflatoxin biosynthesis’ pathways were significantly enriched. Gibberellins and γ-aminobutyric acid were important in the recovery of A. fibrillifera growth after rehydration. Many genes related to antibiotics, vitamins, and other health-related ingredients were found in A. fibrillifera. Conclusion These findings suggested that the candidate genes and metabolites involved in crucial biological pathways might regulate the drought tolerance or rehydration of Auricularia, shedding light on the corresponding mechanisms and providing new potential targets for the breeding and cultivation of drought-tolerant fungi.https://doi.org/10.1186/s12864-021-08284-9Auricularia fibrilliferaTranscriptome analysisDrought stressRehydrationMelaninCaffeine |
| spellingShingle | Yiqin Wang Zhifen Yang Luxi Shi Rui Yang Hao Guo Suqin Zhang Guangdong Geng Transcriptome analysis of Auricularia fibrillifera fruit-body responses to drought stress and rehydration BMC Genomics Auricularia fibrillifera Transcriptome analysis Drought stress Rehydration Melanin Caffeine |
| title | Transcriptome analysis of Auricularia fibrillifera fruit-body responses to drought stress and rehydration |
| title_full | Transcriptome analysis of Auricularia fibrillifera fruit-body responses to drought stress and rehydration |
| title_fullStr | Transcriptome analysis of Auricularia fibrillifera fruit-body responses to drought stress and rehydration |
| title_full_unstemmed | Transcriptome analysis of Auricularia fibrillifera fruit-body responses to drought stress and rehydration |
| title_short | Transcriptome analysis of Auricularia fibrillifera fruit-body responses to drought stress and rehydration |
| title_sort | transcriptome analysis of auricularia fibrillifera fruit body responses to drought stress and rehydration |
| topic | Auricularia fibrillifera Transcriptome analysis Drought stress Rehydration Melanin Caffeine |
| url | https://doi.org/10.1186/s12864-021-08284-9 |
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