Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells
We analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulat...
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MDPI AG
2022-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/12/11/1827 |
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| author | Wei Wang Elena Di Nisio Valerio Licursi Emanuele Cacci Giuseppe Lupo Zaal Kokaia Sergio Galanti Paolo Degan Sara D’Angelo Patrizio Castagnola Sara Tavella Rodolfo Negri |
| author_facet | Wei Wang Elena Di Nisio Valerio Licursi Emanuele Cacci Giuseppe Lupo Zaal Kokaia Sergio Galanti Paolo Degan Sara D’Angelo Patrizio Castagnola Sara Tavella Rodolfo Negri |
| author_sort | Wei Wang |
| collection | DOAJ |
| description | We analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulated microgravity for 24 h showed cell aggregate formation and significant modulation of several genes involved in focal adhesion, cytoskeleton regulation, and cell cycle control. These effects were much more limited in hNSPCs cultured at high density in the same conditions. We also found that some of the genes modulated upon exposure to simulated microgravity showed similar changes in hNSPCs grown without laminin in non-adherent culture conditions under normal gravity. These results suggest that reduced gravity counteracts the interactions of cells with the extracellular matrix, inducing morphological and transcriptional changes that can be observed in low-density cultures. |
| first_indexed | 2024-03-09T18:54:24Z |
| format | Article |
| id | doaj.art-068c6363fc754d738bc440627f36ee8c |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T18:54:24Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-068c6363fc754d738bc440627f36ee8c2023-11-24T05:31:35ZengMDPI AGLife2075-17292022-11-011211182710.3390/life12111827Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor CellsWei Wang0Elena Di Nisio1Valerio Licursi2Emanuele Cacci3Giuseppe Lupo4Zaal Kokaia5Sergio Galanti6Paolo Degan7Sara D’Angelo8Patrizio Castagnola9Sara Tavella10Rodolfo Negri11Department of Biology and Biotechnologies “C. Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnologies “C. Darwin”, Sapienza University of Rome, 00185 Rome, ItalyInstitute of Molecular Biology and Pathology (IBPM), National Research Council (CNR) of Italy c/o Department of Biology and Biotechnologies “C. Darwin”, Sapienza University, 00185 Rome, ItalyDepartment of Biology and Biotechnologies “C. Darwin”, Sapienza University of Rome, 00185 Rome, ItalyDepartment of Biology and Biotechnologies “C. Darwin”, Sapienza University of Rome, 00185 Rome, ItalyLund Stem Cell Center, Department of Clinical Sciences, Lund University, 22184 Lund, SwedenExcise, Custom and Monopolies Agency, ADM, 00153 Rome, ItalyIRCCS Ospedale Policlinico San Martino, 16132 Genova, ItalyDepartment of Biology and Biotechnologies “C. Darwin”, Sapienza University of Rome, 00185 Rome, ItalyIRCCS Ospedale Policlinico San Martino, 16132 Genova, ItalyIRCCS Ospedale Policlinico San Martino, 16132 Genova, ItalyDepartment of Biology and Biotechnologies “C. Darwin”, Sapienza University of Rome, 00185 Rome, ItalyWe analyzed the morphology and the transcriptomic changes of human neural stem progenitor cells (hNSPCs) grown on laminin in adherent culture conditions and subjected to simulated microgravity for different times in a random positioning machine apparatus. Low-cell-density cultures exposed to simulated microgravity for 24 h showed cell aggregate formation and significant modulation of several genes involved in focal adhesion, cytoskeleton regulation, and cell cycle control. These effects were much more limited in hNSPCs cultured at high density in the same conditions. We also found that some of the genes modulated upon exposure to simulated microgravity showed similar changes in hNSPCs grown without laminin in non-adherent culture conditions under normal gravity. These results suggest that reduced gravity counteracts the interactions of cells with the extracellular matrix, inducing morphological and transcriptional changes that can be observed in low-density cultures.https://www.mdpi.com/2075-1729/12/11/1827microgravityspace conditionshuman neural stem progenitor cellsfocal adhesionlaminin |
| spellingShingle | Wei Wang Elena Di Nisio Valerio Licursi Emanuele Cacci Giuseppe Lupo Zaal Kokaia Sergio Galanti Paolo Degan Sara D’Angelo Patrizio Castagnola Sara Tavella Rodolfo Negri Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells Life microgravity space conditions human neural stem progenitor cells focal adhesion laminin |
| title | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
| title_full | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
| title_fullStr | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
| title_full_unstemmed | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
| title_short | Simulated Microgravity Modulates Focal Adhesion Gene Expression in Human Neural Stem Progenitor Cells |
| title_sort | simulated microgravity modulates focal adhesion gene expression in human neural stem progenitor cells |
| topic | microgravity space conditions human neural stem progenitor cells focal adhesion laminin |
| url | https://www.mdpi.com/2075-1729/12/11/1827 |
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