Functionalization of a Cortical Membrane with a Photodynamic Protocol
Guided bone regeneration (GBR) comprehends the application of membranes to drive bone healing and to exclude non-osteogenic tissues from interfering with bone regeneration. However, the membranes may be exposed to bacterial attack, with the risk of failure of the GBR. Recently, an antibacterial phot...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/14/3/133 |
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| author | Tania Vanessa Pierfelice Emira D’Amico Simonetta D’Ercole Stefania Lepore Adriano Piattelli Antonio Barone Giovanna Iezzi Morena Petrini |
| author_facet | Tania Vanessa Pierfelice Emira D’Amico Simonetta D’Ercole Stefania Lepore Adriano Piattelli Antonio Barone Giovanna Iezzi Morena Petrini |
| author_sort | Tania Vanessa Pierfelice |
| collection | DOAJ |
| description | Guided bone regeneration (GBR) comprehends the application of membranes to drive bone healing and to exclude non-osteogenic tissues from interfering with bone regeneration. However, the membranes may be exposed to bacterial attack, with the risk of failure of the GBR. Recently, an antibacterial photodynamic protocol (ALAD-PDT) based on a gel with 5% 5-aminolevulinic acid incubated for 45 min and irradiated for 7 min by a LED light at 630 nm, also showed a pro-proliferative effect on human fibroblasts and osteoblasts. The present study hypothesized that the functionalization of a porcine cortical membrane (soft-curved lamina, OsteoBiol) with ALAD-PDT might promote its osteoconductive properties. TEST 1 aimed to verify the response of osteoblasts seeded on lamina with respect to the plate surface (CTRL). TEST 2 aimed to investigate the effects of ALAD-PDT on the osteoblasts cultured on the lamina. SEM analyses were performed to study the topographical characteristics of the membrane surface, the adhesion, and the morphology of cells at 3 days. The viability was assessed at 3 days, the ALP activity at 7 days, and calcium deposition at 14 days. Results showed the porous surface of the lamina and the increase in cell attachment of osteoblasts with respect to controls. The proliferation, the ALP, and bone mineralization activity of osteoblasts seeded on lamina resulted in being significantly higher (<i>p</i> < 0.0001) than controls. Results also showed an additional significative enhancement (<i>p</i> < 0.0001) in the proliferative rate in ALP and calcium deposition after applying ALAD-PDT. In conclusion, the functionalization of the cortical membranes cultured with osteoblasts with the ALAD-PDT improved their osteoconductive properties. |
| first_indexed | 2024-03-11T06:20:44Z |
| format | Article |
| id | doaj.art-068f09dd1f3c4e77b9b7e54637059a0c |
| institution | Directory Open Access Journal |
| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-11T06:20:44Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-068f09dd1f3c4e77b9b7e54637059a0c2023-11-17T11:54:03ZengMDPI AGJournal of Functional Biomaterials2079-49832023-02-0114313310.3390/jfb14030133Functionalization of a Cortical Membrane with a Photodynamic ProtocolTania Vanessa Pierfelice0Emira D’Amico1Simonetta D’Ercole2Stefania Lepore3Adriano Piattelli4Antonio Barone5Giovanna Iezzi6Morena Petrini7Department of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, ItalySchool of Dentistry, Saint Camillus International University of Health and Medical Sciences, Via di Sant’Alessandro 8, 00131 Rome, ItalyDepartment of Surgical, Medical, Molecular Pathologies and of the Critical Needs, School of Dentistry, University of Pisa, 56100 Pisa, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences, University “G. d’Annunzio” of Chieti-Pescara, Via dei Vestini 31, 66013 Chieti, ItalyGuided bone regeneration (GBR) comprehends the application of membranes to drive bone healing and to exclude non-osteogenic tissues from interfering with bone regeneration. However, the membranes may be exposed to bacterial attack, with the risk of failure of the GBR. Recently, an antibacterial photodynamic protocol (ALAD-PDT) based on a gel with 5% 5-aminolevulinic acid incubated for 45 min and irradiated for 7 min by a LED light at 630 nm, also showed a pro-proliferative effect on human fibroblasts and osteoblasts. The present study hypothesized that the functionalization of a porcine cortical membrane (soft-curved lamina, OsteoBiol) with ALAD-PDT might promote its osteoconductive properties. TEST 1 aimed to verify the response of osteoblasts seeded on lamina with respect to the plate surface (CTRL). TEST 2 aimed to investigate the effects of ALAD-PDT on the osteoblasts cultured on the lamina. SEM analyses were performed to study the topographical characteristics of the membrane surface, the adhesion, and the morphology of cells at 3 days. The viability was assessed at 3 days, the ALP activity at 7 days, and calcium deposition at 14 days. Results showed the porous surface of the lamina and the increase in cell attachment of osteoblasts with respect to controls. The proliferation, the ALP, and bone mineralization activity of osteoblasts seeded on lamina resulted in being significantly higher (<i>p</i> < 0.0001) than controls. Results also showed an additional significative enhancement (<i>p</i> < 0.0001) in the proliferative rate in ALP and calcium deposition after applying ALAD-PDT. In conclusion, the functionalization of the cortical membranes cultured with osteoblasts with the ALAD-PDT improved their osteoconductive properties.https://www.mdpi.com/2079-4983/14/3/133lamina cortical membranephotodynamic therapyosteoblasts5-aminolevulinic acidLEDALP |
| spellingShingle | Tania Vanessa Pierfelice Emira D’Amico Simonetta D’Ercole Stefania Lepore Adriano Piattelli Antonio Barone Giovanna Iezzi Morena Petrini Functionalization of a Cortical Membrane with a Photodynamic Protocol Journal of Functional Biomaterials lamina cortical membrane photodynamic therapy osteoblasts 5-aminolevulinic acid LED ALP |
| title | Functionalization of a Cortical Membrane with a Photodynamic Protocol |
| title_full | Functionalization of a Cortical Membrane with a Photodynamic Protocol |
| title_fullStr | Functionalization of a Cortical Membrane with a Photodynamic Protocol |
| title_full_unstemmed | Functionalization of a Cortical Membrane with a Photodynamic Protocol |
| title_short | Functionalization of a Cortical Membrane with a Photodynamic Protocol |
| title_sort | functionalization of a cortical membrane with a photodynamic protocol |
| topic | lamina cortical membrane photodynamic therapy osteoblasts 5-aminolevulinic acid LED ALP |
| url | https://www.mdpi.com/2079-4983/14/3/133 |
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