Metabolite profiling and nephroprotective potential of Glycyrrhiza glabra L. roots against cisplatin-induced nephrotoxicity in vitro and in vivo
Objective(s): The present study was conducted to investigate the phytochemical analysis and demonstrate the nephroprotective potential of root extract of Glycyrrhiza glabra L. against cisplatin (CP) -induced nephrotoxicity in vitro and in vivo. Materials and Methods: The HPTLC analysis and UPLC-MS w...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-11-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | https://ijbms.mums.ac.ir/article_21121_9c79d9a9af40847ac142cc596b304d47.pdf |
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| author | Parakh Basist Sultann Zahiruddin Mohammad Khan Gaurav Gautam Bisma Jan Mohammad Khan Rabea Parveen Sayeed Ahmad |
| author_facet | Parakh Basist Sultann Zahiruddin Mohammad Khan Gaurav Gautam Bisma Jan Mohammad Khan Rabea Parveen Sayeed Ahmad |
| author_sort | Parakh Basist |
| collection | DOAJ |
| description | Objective(s): The present study was conducted to investigate the phytochemical analysis and demonstrate the nephroprotective potential of root extract of Glycyrrhiza glabra L. against cisplatin (CP) -induced nephrotoxicity in vitro and in vivo. Materials and Methods: The HPTLC analysis and UPLC-MS were carried out for standardizing and metabolite profiling of methanolic extract of roots of G. glabra (GGE). Further, in vitro studies were conducted in human embryonic kidney (HEK)-293 cells to evaluate the cytotoxicity and anti-oxidant potential of GGE with CP as a toxicant and ascorbic acid as standard. Also, in vivo nephroprotective potential at doses of 31.5, 63, and 126 mg/kg/day on CP (6 mg/kg, bw, IP) induced nephrotoxicity was evaluated on rodents. Results: Phytochemical analysis by HPTLC and UPLC-MS revealed the presence of glycyrrhizin, glabridin, and liquiritin along with other bioactive constituents. The in vitro assay of GGE showed significant (P<0.001 nephroprotective, cellular anti-oxidant potential and improvement in morphological changes induced by CP. Further, administration of CP caused significant (P<0.001) elevation in biochemical, inflammatory, oxidative stress, caspase-3, as well as histopathological changes in kidney tissue. Pre-treatment with GGE attenuated the elevated biochemical markers significantly, improved histopathological damage, and showed a comparable result to ascorbic acid and α-ketoanalogue. Conclusion: Present study concluded the nephroprotective potential of GGE which supports the traditional claim of G. glabra roots in various kidney and its related disorders. The nephroprotective activity may be attributed to its anti-oxidant, anti-inflammatory, and anti-apoptosis effects. Thus, it holds promising potential in management of nephrotoxicity. |
| first_indexed | 2024-04-11T23:54:45Z |
| format | Article |
| id | doaj.art-0691778fbe5e43aabf678b0a4d5cdced |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-11T23:54:45Z |
| publishDate | 2022-11-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-0691778fbe5e43aabf678b0a4d5cdced2022-12-22T03:56:24ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-11-0125111286129810.22038/ijbms.2022.65478.1440421121Metabolite profiling and nephroprotective potential of Glycyrrhiza glabra L. roots against cisplatin-induced nephrotoxicity in vitro and in vivoParakh Basist0Sultann Zahiruddin1Mohammad Khan2Gaurav Gautam3Bisma Jan4Mohammad Khan5Rabea Parveen6Sayeed Ahmad7Centre of Excellence in Unani Medicine (Pharmacognosy and Pharmacology), Jamia Hamdard, New Delhi, India-110062Centre of Excellence in Unani Medicine (Pharmacognosy and Pharmacology), Jamia Hamdard, New Delhi, India-110062Centre of Excellence in Unani Medicine (Pharmacognosy and Pharmacology), Jamia Hamdard, New Delhi, India-110062Centre of Excellence in Unani Medicine (Pharmacognosy and Pharmacology), Jamia Hamdard, New Delhi, India-110062Centre of Excellence in Unani Medicine (Pharmacognosy and Pharmacology), Jamia Hamdard, New Delhi, India-110062Department of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India-110062Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India-110062Centre of Excellence in Unani Medicine (Pharmacognosy and Pharmacology), Jamia Hamdard, New Delhi, India-110062Objective(s): The present study was conducted to investigate the phytochemical analysis and demonstrate the nephroprotective potential of root extract of Glycyrrhiza glabra L. against cisplatin (CP) -induced nephrotoxicity in vitro and in vivo. Materials and Methods: The HPTLC analysis and UPLC-MS were carried out for standardizing and metabolite profiling of methanolic extract of roots of G. glabra (GGE). Further, in vitro studies were conducted in human embryonic kidney (HEK)-293 cells to evaluate the cytotoxicity and anti-oxidant potential of GGE with CP as a toxicant and ascorbic acid as standard. Also, in vivo nephroprotective potential at doses of 31.5, 63, and 126 mg/kg/day on CP (6 mg/kg, bw, IP) induced nephrotoxicity was evaluated on rodents. Results: Phytochemical analysis by HPTLC and UPLC-MS revealed the presence of glycyrrhizin, glabridin, and liquiritin along with other bioactive constituents. The in vitro assay of GGE showed significant (P<0.001 nephroprotective, cellular anti-oxidant potential and improvement in morphological changes induced by CP. Further, administration of CP caused significant (P<0.001) elevation in biochemical, inflammatory, oxidative stress, caspase-3, as well as histopathological changes in kidney tissue. Pre-treatment with GGE attenuated the elevated biochemical markers significantly, improved histopathological damage, and showed a comparable result to ascorbic acid and α-ketoanalogue. Conclusion: Present study concluded the nephroprotective potential of GGE which supports the traditional claim of G. glabra roots in various kidney and its related disorders. The nephroprotective activity may be attributed to its anti-oxidant, anti-inflammatory, and anti-apoptosis effects. Thus, it holds promising potential in management of nephrotoxicity.https://ijbms.mums.ac.ir/article_21121_9c79d9a9af40847ac142cc596b304d47.pdfcisplatinglycyrrhizinglycyrrihza glabrakidney disordernephroprotectivenephrotoxicity |
| spellingShingle | Parakh Basist Sultann Zahiruddin Mohammad Khan Gaurav Gautam Bisma Jan Mohammad Khan Rabea Parveen Sayeed Ahmad Metabolite profiling and nephroprotective potential of Glycyrrhiza glabra L. roots against cisplatin-induced nephrotoxicity in vitro and in vivo Iranian Journal of Basic Medical Sciences cisplatin glycyrrhizin glycyrrihza glabra kidney disorder nephroprotective nephrotoxicity |
| title | Metabolite profiling and nephroprotective potential of Glycyrrhiza glabra L. roots against cisplatin-induced nephrotoxicity in vitro and in vivo |
| title_full | Metabolite profiling and nephroprotective potential of Glycyrrhiza glabra L. roots against cisplatin-induced nephrotoxicity in vitro and in vivo |
| title_fullStr | Metabolite profiling and nephroprotective potential of Glycyrrhiza glabra L. roots against cisplatin-induced nephrotoxicity in vitro and in vivo |
| title_full_unstemmed | Metabolite profiling and nephroprotective potential of Glycyrrhiza glabra L. roots against cisplatin-induced nephrotoxicity in vitro and in vivo |
| title_short | Metabolite profiling and nephroprotective potential of Glycyrrhiza glabra L. roots against cisplatin-induced nephrotoxicity in vitro and in vivo |
| title_sort | metabolite profiling and nephroprotective potential of glycyrrhiza glabra l roots against cisplatin induced nephrotoxicity in vitro and in vivo |
| topic | cisplatin glycyrrhizin glycyrrihza glabra kidney disorder nephroprotective nephrotoxicity |
| url | https://ijbms.mums.ac.ir/article_21121_9c79d9a9af40847ac142cc596b304d47.pdf |
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