Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches.
Our current taxonomic perspective on Entamoeba is largely based on small-subunit ribosomal RNA genes (SSU rDNA) from Entamoeba species identified in vertebrate hosts with minor exceptions such as E. moshkovskii from sewage water and E. marina from marine sediment. Other Entamoeba species have also b...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5608334?pdf=render |
| _version_ | 1819120350703648768 |
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| author | Tetsuro Kawano Mihoko Imada Pennapa Chamavit Seiki Kobayashi Tetsuo Hashimoto Tomoyoshi Nozaki |
| author_facet | Tetsuro Kawano Mihoko Imada Pennapa Chamavit Seiki Kobayashi Tetsuo Hashimoto Tomoyoshi Nozaki |
| author_sort | Tetsuro Kawano |
| collection | DOAJ |
| description | Our current taxonomic perspective on Entamoeba is largely based on small-subunit ribosomal RNA genes (SSU rDNA) from Entamoeba species identified in vertebrate hosts with minor exceptions such as E. moshkovskii from sewage water and E. marina from marine sediment. Other Entamoeba species have also been morphologically identified and described from non-vertebrate species such as insects; however, their genetic diversity remains unknown. In order to further disclose the diversity of the genus, we investigated Entamoeba spp. in the intestines of three cockroach species: Periplaneta americana, Blaptica dubia, and Gromphadorhina oblongonota. We obtained 134 Entamoeba SSU rDNA sequences from 186 cockroaches by direct nested PCR using the DNA extracts of intestines from cockroaches, followed by scrutinized BLASTn screening and phylogenetic analyses. All the sequences identified in this study were distinct from those reported from known Entamoeba species, and considered as novel Entamoeba ribosomal lineages. Furthermore, they were positioned at the base of the clade of known Entamoeba species and displayed remarkable degree of genetic diversity comprising nine major groups in the three cockroach species. This is the first report of the diversity of SSU rDNA sequences from Entamoeba in non-vertebrate host species, and should help to understand the genetic diversity of the genus Entamoeba. |
| first_indexed | 2024-12-22T06:19:16Z |
| format | Article |
| id | doaj.art-06923b949ebe456ab4427c8ab31d3227 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T06:19:16Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-06923b949ebe456ab4427c8ab31d32272022-12-21T18:35:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01129e018523310.1371/journal.pone.0185233Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches.Tetsuro KawanoMihoko ImadaPennapa ChamavitSeiki KobayashiTetsuo HashimotoTomoyoshi NozakiOur current taxonomic perspective on Entamoeba is largely based on small-subunit ribosomal RNA genes (SSU rDNA) from Entamoeba species identified in vertebrate hosts with minor exceptions such as E. moshkovskii from sewage water and E. marina from marine sediment. Other Entamoeba species have also been morphologically identified and described from non-vertebrate species such as insects; however, their genetic diversity remains unknown. In order to further disclose the diversity of the genus, we investigated Entamoeba spp. in the intestines of three cockroach species: Periplaneta americana, Blaptica dubia, and Gromphadorhina oblongonota. We obtained 134 Entamoeba SSU rDNA sequences from 186 cockroaches by direct nested PCR using the DNA extracts of intestines from cockroaches, followed by scrutinized BLASTn screening and phylogenetic analyses. All the sequences identified in this study were distinct from those reported from known Entamoeba species, and considered as novel Entamoeba ribosomal lineages. Furthermore, they were positioned at the base of the clade of known Entamoeba species and displayed remarkable degree of genetic diversity comprising nine major groups in the three cockroach species. This is the first report of the diversity of SSU rDNA sequences from Entamoeba in non-vertebrate host species, and should help to understand the genetic diversity of the genus Entamoeba.http://europepmc.org/articles/PMC5608334?pdf=render |
| spellingShingle | Tetsuro Kawano Mihoko Imada Pennapa Chamavit Seiki Kobayashi Tetsuo Hashimoto Tomoyoshi Nozaki Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches. PLoS ONE |
| title | Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches. |
| title_full | Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches. |
| title_fullStr | Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches. |
| title_full_unstemmed | Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches. |
| title_short | Genetic diversity of Entamoeba: Novel ribosomal lineages from cockroaches. |
| title_sort | genetic diversity of entamoeba novel ribosomal lineages from cockroaches |
| url | http://europepmc.org/articles/PMC5608334?pdf=render |
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