The influence of dehydroepiandrosterone on effector functions of neutrophils
Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the i...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo
2021-11-01
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| Series: | Brazilian Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502021000100544&tlng=en |
| _version_ | 1811271327627083776 |
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| author | Verônica Soares Brauer Fabiana Albani Zambuzi Milena Sobral Espíndola Marinaldo Pacífico Cavalcanti Neto Morgana Kelly Borges Prado Priscilla Mariane Cardoso Luana Silva Soares Leonardo Judson Galvao-Lima Andréia Machado Leopoldino Cristina Ribeiro de Barros Cardoso Fabiani Gai Frantz |
| author_facet | Verônica Soares Brauer Fabiana Albani Zambuzi Milena Sobral Espíndola Marinaldo Pacífico Cavalcanti Neto Morgana Kelly Borges Prado Priscilla Mariane Cardoso Luana Silva Soares Leonardo Judson Galvao-Lima Andréia Machado Leopoldino Cristina Ribeiro de Barros Cardoso Fabiani Gai Frantz |
| author_sort | Verônica Soares Brauer |
| collection | DOAJ |
| description | Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 μM of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap (NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NFκB were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils. |
| first_indexed | 2024-04-12T22:19:12Z |
| format | Article |
| id | doaj.art-06937d099f6241979384a52294f4a015 |
| institution | Directory Open Access Journal |
| issn | 2175-9790 |
| language | English |
| last_indexed | 2024-04-12T22:19:12Z |
| publishDate | 2021-11-01 |
| publisher | Universidade de São Paulo |
| record_format | Article |
| series | Brazilian Journal of Pharmaceutical Sciences |
| spelling | doaj.art-06937d099f6241979384a52294f4a0152022-12-22T03:14:26ZengUniversidade de São PauloBrazilian Journal of Pharmaceutical Sciences2175-97902021-11-015710.1590/s2175-97902020000419139The influence of dehydroepiandrosterone on effector functions of neutrophilsVerônica Soares BrauerFabiana Albani ZambuziMilena Sobral EspíndolaMarinaldo Pacífico Cavalcanti NetoMorgana Kelly Borges PradoPriscilla Mariane CardosoLuana Silva SoaresLeonardo Judson Galvao-LimaAndréia Machado LeopoldinoCristina Ribeiro de Barros CardosoFabiani Gai Frantzhttps://orcid.org/0000-0001-6960-2438Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 μM of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap (NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NFκB were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502021000100544&tlng=enDehydroepiandrosteroneNeuroimmunoendocrinologyS. typhimuriumInnate immunityPhagocytosis |
| spellingShingle | Verônica Soares Brauer Fabiana Albani Zambuzi Milena Sobral Espíndola Marinaldo Pacífico Cavalcanti Neto Morgana Kelly Borges Prado Priscilla Mariane Cardoso Luana Silva Soares Leonardo Judson Galvao-Lima Andréia Machado Leopoldino Cristina Ribeiro de Barros Cardoso Fabiani Gai Frantz The influence of dehydroepiandrosterone on effector functions of neutrophils Brazilian Journal of Pharmaceutical Sciences Dehydroepiandrosterone Neuroimmunoendocrinology S. typhimurium Innate immunity Phagocytosis |
| title | The influence of dehydroepiandrosterone on effector functions of neutrophils |
| title_full | The influence of dehydroepiandrosterone on effector functions of neutrophils |
| title_fullStr | The influence of dehydroepiandrosterone on effector functions of neutrophils |
| title_full_unstemmed | The influence of dehydroepiandrosterone on effector functions of neutrophils |
| title_short | The influence of dehydroepiandrosterone on effector functions of neutrophils |
| title_sort | influence of dehydroepiandrosterone on effector functions of neutrophils |
| topic | Dehydroepiandrosterone Neuroimmunoendocrinology S. typhimurium Innate immunity Phagocytosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502021000100544&tlng=en |
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