In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells
Human amniotic epithelial cells (hAECs) represent an interesting clinical alternative to human embryonic (hESCs) and induced pluripotent (hiPSCs) stem cells in regenerative medicine. The potential of hAECs can be enhanced ex vivo by their partial pre-differentiation. The aim of this study was to eva...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/14/2138 |
| _version_ | 1797407013685166080 |
|---|---|
| author | Marcin Michalik Patrycja Wieczorek Piotr Czekaj |
| author_facet | Marcin Michalik Patrycja Wieczorek Piotr Czekaj |
| author_sort | Marcin Michalik |
| collection | DOAJ |
| description | Human amniotic epithelial cells (hAECs) represent an interesting clinical alternative to human embryonic (hESCs) and induced pluripotent (hiPSCs) stem cells in regenerative medicine. The potential of hAECs can be enhanced ex vivo by their partial pre-differentiation. The aim of this study was to evaluate the effectiveness of 18-day differentiation of hAECs into endodermal cells, hepatic precursor cells, and cells showing functional features of hepatocytes using culture media supplemented with high (100 ng/mL) concentrations of EGF or HGF. The cells obtained after differentiation showed changes in morphology and increased expression of <i>AFP</i>, <i>ALB</i>, <i>CYP3A4</i>, <i>CYP3A7</i>, and <i>GSTP1</i> genes. HGF was more effective than EGF in increasing the expression of liver-specific genes in hAECs. However, EGF stimulated the differentiation process more efficiently and yielded more hepatocyte-like cells capable of synthesizing α-fetoprotein during differentiation. Additionally, after 18 days, GST transferases, albumin, and CYP P450s, which proved their partial functionality, were expressed. In summary, HGF and EGF at a dose of 100 ng/mL can be successfully used to obtain hepatocyte-like cells between days 7 and 18 of hAEC differentiation. However, the effectiveness of this process is lower compared with hiPSC differentiation; therefore, optimization of the composition of the medium requires further research. |
| first_indexed | 2024-03-09T03:35:08Z |
| format | Article |
| id | doaj.art-0696c3793de148b7a7afa1c96b3e690c |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T03:35:08Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-0696c3793de148b7a7afa1c96b3e690c2023-12-03T14:49:27ZengMDPI AGCells2073-44092022-07-011114213810.3390/cells11142138In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like CellsMarcin Michalik0Patrycja Wieczorek1Piotr Czekaj2Department of Cytophysiology, Chair of Histology and Embryology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, 40-752 Katowice, PolandDepartment of Cytophysiology, Chair of Histology and Embryology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, 40-752 Katowice, PolandDepartment of Cytophysiology, Chair of Histology and Embryology, Faculty of Medical Sciences in Katowice, Medical University of Silesia in Katowice, 40-752 Katowice, PolandHuman amniotic epithelial cells (hAECs) represent an interesting clinical alternative to human embryonic (hESCs) and induced pluripotent (hiPSCs) stem cells in regenerative medicine. The potential of hAECs can be enhanced ex vivo by their partial pre-differentiation. The aim of this study was to evaluate the effectiveness of 18-day differentiation of hAECs into endodermal cells, hepatic precursor cells, and cells showing functional features of hepatocytes using culture media supplemented with high (100 ng/mL) concentrations of EGF or HGF. The cells obtained after differentiation showed changes in morphology and increased expression of <i>AFP</i>, <i>ALB</i>, <i>CYP3A4</i>, <i>CYP3A7</i>, and <i>GSTP1</i> genes. HGF was more effective than EGF in increasing the expression of liver-specific genes in hAECs. However, EGF stimulated the differentiation process more efficiently and yielded more hepatocyte-like cells capable of synthesizing α-fetoprotein during differentiation. Additionally, after 18 days, GST transferases, albumin, and CYP P450s, which proved their partial functionality, were expressed. In summary, HGF and EGF at a dose of 100 ng/mL can be successfully used to obtain hepatocyte-like cells between days 7 and 18 of hAEC differentiation. However, the effectiveness of this process is lower compared with hiPSC differentiation; therefore, optimization of the composition of the medium requires further research.https://www.mdpi.com/2073-4409/11/14/2138amniotic epithelial cellsstem cellsdifferentiationhepatocytesEGFHGF |
| spellingShingle | Marcin Michalik Patrycja Wieczorek Piotr Czekaj In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells Cells amniotic epithelial cells stem cells differentiation hepatocytes EGF HGF |
| title | In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells |
| title_full | In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells |
| title_fullStr | In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells |
| title_full_unstemmed | In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells |
| title_short | In Vitro Differentiation of Human Amniotic Epithelial Cells into Hepatocyte-like Cells |
| title_sort | in vitro differentiation of human amniotic epithelial cells into hepatocyte like cells |
| topic | amniotic epithelial cells stem cells differentiation hepatocytes EGF HGF |
| url | https://www.mdpi.com/2073-4409/11/14/2138 |
| work_keys_str_mv | AT marcinmichalik invitrodifferentiationofhumanamnioticepithelialcellsintohepatocytelikecells AT patrycjawieczorek invitrodifferentiationofhumanamnioticepithelialcellsintohepatocytelikecells AT piotrczekaj invitrodifferentiationofhumanamnioticepithelialcellsintohepatocytelikecells |