Altered epithelial barrier functions in the colon of patients with spina bifida
Abstract Our objectives were to better characterize the colorectal function of patients with Spina Bifida (SB). Patients with SB and healthy volunteers (HVs) completed prospectively a standardized questionnaire, clinical evaluation, rectal barostat, colonoscopy with biopsies and faecal collection. T...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-05-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-11289-3 |
| _version_ | 1811338784545964032 |
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| author | Charlène Brochard Guillaume Bouguen Raphael Olivier Tony Durand Sébastien Henno Benoît Peyronnet Mael Pagenault Chloé Lefèvre Gaëlle Boudry Mikael Croyal Alain Fautrel Maxime Esvan Alain Ropert Anne Dariel Laurent Siproudhis Michel Neunlist |
| author_facet | Charlène Brochard Guillaume Bouguen Raphael Olivier Tony Durand Sébastien Henno Benoît Peyronnet Mael Pagenault Chloé Lefèvre Gaëlle Boudry Mikael Croyal Alain Fautrel Maxime Esvan Alain Ropert Anne Dariel Laurent Siproudhis Michel Neunlist |
| author_sort | Charlène Brochard |
| collection | DOAJ |
| description | Abstract Our objectives were to better characterize the colorectal function of patients with Spina Bifida (SB). Patients with SB and healthy volunteers (HVs) completed prospectively a standardized questionnaire, clinical evaluation, rectal barostat, colonoscopy with biopsies and faecal collection. The data from 36 adults with SB (age: 38.8 [34.1–47.2]) were compared with those of 16 HVs (age: 39.0 [31.0–46.5]). Compared to HVs, rectal compliance was lower in patients with SB (p = 0.01), whereas rectal tone was higher (p = 0.0015). Ex vivo paracellular permeability was increased in patients with SB (p = 0.0008) and inversely correlated with rectal compliance (r = − 0.563, p = 0.002). The expression of key tight junction proteins and inflammatory markers was comparable between SB and HVs, except for an increase in Claudin-1 immunoreactivity (p = 0.04) in SB compared to HVs. TGFβ1 and GDNF mRNAs were expressed at higher levels in patients with SB (p = 0.02 and p = 0.008). The levels of acetate, propionate and butyrate in faecal samples were reduced (p = 0.04, p = 0.01, and p = 0.02, respectively). Our findings provide evidence that anorectal and epithelial functions are altered in patients with SB. The alterations in these key functions might represent new therapeutic targets, in particular using microbiota-derived approaches. Clinical Trials: NCT02440984 and NCT03054415. |
| first_indexed | 2024-04-13T18:15:46Z |
| format | Article |
| id | doaj.art-0698708084f5404f9613685d4f354a91 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-13T18:15:46Z |
| publishDate | 2022-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-0698708084f5404f9613685d4f354a912022-12-22T02:35:41ZengNature PortfolioScientific Reports2045-23222022-05-0112111210.1038/s41598-022-11289-3Altered epithelial barrier functions in the colon of patients with spina bifidaCharlène Brochard0Guillaume Bouguen1Raphael Olivier2Tony Durand3Sébastien Henno4Benoît Peyronnet5Mael Pagenault6Chloé Lefèvre7Gaëlle Boudry8Mikael Croyal9Alain Fautrel10Maxime Esvan11Alain Ropert12Anne Dariel13Laurent Siproudhis14Michel Neunlist15Service d’Explorations Fonctionnelles Digestives, CHRU Pontchaillou, Université de Rennes 1Inserm, CIC 1414 (Centre d’Investigation Clinique de Rennes), CHU RennesThe Enteric Nervous System in Gut and Brain Disorders INSERM, TENS, Université de NantesThe Enteric Nervous System in Gut and Brain Disorders INSERM, TENS, Université de NantesService d’Anatomopathologie, CHRU PontchaillouInserm, CIC 1414 (Centre d’Investigation Clinique de Rennes), CHU RennesService des Maladies de l’Appareil Digestif, CHRU Pontchaillou, Université de Rennes 1The Enteric Nervous System in Gut and Brain Disorders INSERM, TENS, Université de NantesInstitut Numecan, INSERM, INRAE, Univ RennesUniversité de Nantes, CHU Nantes, INSERM, CNRS, SFR Santé, Inserm UMS 016, CNRS UMS 3556Plateforme H2P2, Université de RennesInserm, CIC 1414 (Centre d’Investigation Clinique de Rennes), CHU RennesService d’Explorations Fonctionnelles Digestives, CHRU Pontchaillou, Université de Rennes 1Service de Chirurgie Pédiatrique, CHU MarseilleInserm, CIC 1414 (Centre d’Investigation Clinique de Rennes), CHU RennesThe Enteric Nervous System in Gut and Brain Disorders INSERM, TENS, Université de NantesAbstract Our objectives were to better characterize the colorectal function of patients with Spina Bifida (SB). Patients with SB and healthy volunteers (HVs) completed prospectively a standardized questionnaire, clinical evaluation, rectal barostat, colonoscopy with biopsies and faecal collection. The data from 36 adults with SB (age: 38.8 [34.1–47.2]) were compared with those of 16 HVs (age: 39.0 [31.0–46.5]). Compared to HVs, rectal compliance was lower in patients with SB (p = 0.01), whereas rectal tone was higher (p = 0.0015). Ex vivo paracellular permeability was increased in patients with SB (p = 0.0008) and inversely correlated with rectal compliance (r = − 0.563, p = 0.002). The expression of key tight junction proteins and inflammatory markers was comparable between SB and HVs, except for an increase in Claudin-1 immunoreactivity (p = 0.04) in SB compared to HVs. TGFβ1 and GDNF mRNAs were expressed at higher levels in patients with SB (p = 0.02 and p = 0.008). The levels of acetate, propionate and butyrate in faecal samples were reduced (p = 0.04, p = 0.01, and p = 0.02, respectively). Our findings provide evidence that anorectal and epithelial functions are altered in patients with SB. The alterations in these key functions might represent new therapeutic targets, in particular using microbiota-derived approaches. Clinical Trials: NCT02440984 and NCT03054415.https://doi.org/10.1038/s41598-022-11289-3 |
| spellingShingle | Charlène Brochard Guillaume Bouguen Raphael Olivier Tony Durand Sébastien Henno Benoît Peyronnet Mael Pagenault Chloé Lefèvre Gaëlle Boudry Mikael Croyal Alain Fautrel Maxime Esvan Alain Ropert Anne Dariel Laurent Siproudhis Michel Neunlist Altered epithelial barrier functions in the colon of patients with spina bifida Scientific Reports |
| title | Altered epithelial barrier functions in the colon of patients with spina bifida |
| title_full | Altered epithelial barrier functions in the colon of patients with spina bifida |
| title_fullStr | Altered epithelial barrier functions in the colon of patients with spina bifida |
| title_full_unstemmed | Altered epithelial barrier functions in the colon of patients with spina bifida |
| title_short | Altered epithelial barrier functions in the colon of patients with spina bifida |
| title_sort | altered epithelial barrier functions in the colon of patients with spina bifida |
| url | https://doi.org/10.1038/s41598-022-11289-3 |
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