Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions
Peripheral nerve injury (PNI) is a neurological disorder that causes more than 9 million patients to suffer from dysfunction of moving and sensing. Using biodegradable polymers to fabricate an artificial nerve conduit that replicates the environment of the extracellular matrix and guides neuron rege...
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MDPI AG
2022-05-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/13/2/58 |
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| author | Yansheng Zhou Ying Li Daqing Li Yidan Yin Fenglei Zhou |
| author_facet | Yansheng Zhou Ying Li Daqing Li Yidan Yin Fenglei Zhou |
| author_sort | Yansheng Zhou |
| collection | DOAJ |
| description | Peripheral nerve injury (PNI) is a neurological disorder that causes more than 9 million patients to suffer from dysfunction of moving and sensing. Using biodegradable polymers to fabricate an artificial nerve conduit that replicates the environment of the extracellular matrix and guides neuron regeneration through the damaged sites has been researched for decades and has led to promising but primarily pre-clinical outcomes. However, few peripheral nerve conduits (PNCs) have been constructed from controllable biodegradable polymeric materials that can maintain their structural integrity or completely degrade during and after nerve regeneration respectively. In this work, a novel PNC candidate material was developed via the electrospinning of polyhydroxy butyrate/chitosan (PHB/CS) composite polymers. An SEM characterisation revealed the resultant PHB/CS nanofibres with 0, 1 and 2 wt/v% CS had less and smaller beads than the nanofibres at 3 wt/v% CS. The water contact angle (WCA) measurement demonstrated that the wettability of PHB/CS electrospun fibres was significantly improved by additional CS. Furthermore, both the thermogravimetric analysis (TGA) and differentiation scanning calorimetry (DSC) results showed that PHB/CS polymers can be blended in a single phase with a trifluoracetic solvent in all compositions. Besides, the reduction in the degradation temperature (from 286.9 to 229.9 °C) and crystallinity (from 81.0% to 52.1%) with increasing contents of CS were further proven. Moreover, we found that the degradability of the PHB/CS nanofibres subjected to different pH values rated in the order of acidic > alkaline > phosphate buffer solution (PBS). Based on these findings, it can be concluded that PHB/CS electrospun fibres with variable blending ratios may be used for designing PNCs with controlled biodegradability. |
| first_indexed | 2024-03-09T23:25:00Z |
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| id | doaj.art-069875e670384cd8b85caa9e67158290 |
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| issn | 2079-4983 |
| language | English |
| last_indexed | 2024-03-09T23:25:00Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj.art-069875e670384cd8b85caa9e671582902023-11-23T17:19:29ZengMDPI AGJournal of Functional Biomaterials2079-49832022-05-011325810.3390/jfb13020058Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH ConditionsYansheng Zhou0Ying Li1Daqing Li2Yidan Yin3Fenglei Zhou4Institute for Materials Discovery, Faculty of Mathematical Physical Sciences, University College London, 107 Roberts Building, Malet Place, London WC1E 7JE, UKSpinal Repair Unit, Department of Brain Repair and Rehabilitation, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UKSpinal Repair Unit, Department of Brain Repair and Rehabilitation, UCL Institute of Neurology, Queen Square, London WC1N 3BG, UKDepartment of Chemistry, Christopher Ingold Building, University College London (UCL), 20 Gordon Street, London WC1H 0AJ, UKInstitute for Materials Discovery, Faculty of Mathematical Physical Sciences, University College London, 107 Roberts Building, Malet Place, London WC1E 7JE, UKPeripheral nerve injury (PNI) is a neurological disorder that causes more than 9 million patients to suffer from dysfunction of moving and sensing. Using biodegradable polymers to fabricate an artificial nerve conduit that replicates the environment of the extracellular matrix and guides neuron regeneration through the damaged sites has been researched for decades and has led to promising but primarily pre-clinical outcomes. However, few peripheral nerve conduits (PNCs) have been constructed from controllable biodegradable polymeric materials that can maintain their structural integrity or completely degrade during and after nerve regeneration respectively. In this work, a novel PNC candidate material was developed via the electrospinning of polyhydroxy butyrate/chitosan (PHB/CS) composite polymers. An SEM characterisation revealed the resultant PHB/CS nanofibres with 0, 1 and 2 wt/v% CS had less and smaller beads than the nanofibres at 3 wt/v% CS. The water contact angle (WCA) measurement demonstrated that the wettability of PHB/CS electrospun fibres was significantly improved by additional CS. Furthermore, both the thermogravimetric analysis (TGA) and differentiation scanning calorimetry (DSC) results showed that PHB/CS polymers can be blended in a single phase with a trifluoracetic solvent in all compositions. Besides, the reduction in the degradation temperature (from 286.9 to 229.9 °C) and crystallinity (from 81.0% to 52.1%) with increasing contents of CS were further proven. Moreover, we found that the degradability of the PHB/CS nanofibres subjected to different pH values rated in the order of acidic > alkaline > phosphate buffer solution (PBS). Based on these findings, it can be concluded that PHB/CS electrospun fibres with variable blending ratios may be used for designing PNCs with controlled biodegradability.https://www.mdpi.com/2079-4983/13/2/58electrospinningPolyhydroxybutyrate (PHB)chitosan (CS)pH valuedegradation |
| spellingShingle | Yansheng Zhou Ying Li Daqing Li Yidan Yin Fenglei Zhou Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions Journal of Functional Biomaterials electrospinning Polyhydroxybutyrate (PHB) chitosan (CS) pH value degradation |
| title | Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions |
| title_full | Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions |
| title_fullStr | Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions |
| title_full_unstemmed | Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions |
| title_short | Electrospun PHB/Chitosan Composite Fibrous Membrane and Its Degradation Behaviours in Different pH Conditions |
| title_sort | electrospun phb chitosan composite fibrous membrane and its degradation behaviours in different ph conditions |
| topic | electrospinning Polyhydroxybutyrate (PHB) chitosan (CS) pH value degradation |
| url | https://www.mdpi.com/2079-4983/13/2/58 |
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