Anti-Inflammatory and Antioxidant Properties of <i>Malapterurus electricus</i> Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects
The protective and therapeutic anti-inflammatory and antioxidant potency of <i>Malapterurus electricus</i> (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints....
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MDPI AG
2022-10-01
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| author | Abeer H. Elmaidomy Esraa M. Mohamed Hanan F. Aly Eman A. Younis Shams Gamal Eldin Shams Faisal H. Altemani Mubarak A. Alzubaidi Mohammed Almaghrabi Adnan Al Harbi Faisal Alsenani Ahmed M. Sayed Usama Ramadan Abdelmohsen |
| author_facet | Abeer H. Elmaidomy Esraa M. Mohamed Hanan F. Aly Eman A. Younis Shams Gamal Eldin Shams Faisal H. Altemani Mubarak A. Alzubaidi Mohammed Almaghrabi Adnan Al Harbi Faisal Alsenani Ahmed M. Sayed Usama Ramadan Abdelmohsen |
| author_sort | Abeer H. Elmaidomy |
| collection | DOAJ |
| description | The protective and therapeutic anti-inflammatory and antioxidant potency of <i>Malapterurus electricus</i> (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints. Serum uric acid, TNF-α, IL-1β, NF-𝜅B, MDA, GSH, catalase, SOD, and glutathione reductase levels were all measured. According to the findings, FE significantly reduced uric acid levels and ankle swelling in both protective and therapeutic groups. Furthermore, it has anti-inflammatory effects by downregulating inflammatory cytokines, primarily through decreased oxidative stress and increased antioxidant status. All the aforementioned lesions were significantly improved in protected and treated rats with FE, according to histopathological findings. iNOS immunostaining revealed that protected and treated arthritic rats with FE had weak positive immune-reactive cells. Phytochemical analysis revealed that FE was high in fatty and amino acids. The most abundant compounds were vaccenic (24.52%), 9-octadecenoic (11.66%), palmitic (34.66%), stearic acids (14.63%), glycine (0.813 mg/100 mg), and alanine (1.645 mg/100 mg). Extensive molecular modelling and dynamics simulation experiments revealed that compound <b>4</b> has the potential to target and inhibit COX isoforms with a higher affinity for COX-2. As a result, we contend that FE could be a promising protective and therapeutic option for arthritis, aiding in the prevention and progression of this chronic inflammatory disease. |
| first_indexed | 2024-03-09T19:53:44Z |
| format | Article |
| id | doaj.art-0698e4fee16b46489b0d4409184c7d8d |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T19:53:44Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-0698e4fee16b46489b0d4409184c7d8d2023-11-24T01:01:23ZengMDPI AGMarine Drugs1660-33972022-10-01201063910.3390/md20100639Anti-Inflammatory and Antioxidant Properties of <i>Malapterurus electricus</i> Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective EffectsAbeer H. Elmaidomy0Esraa M. Mohamed1Hanan F. Aly2Eman A. Younis3Shams Gamal Eldin Shams4Faisal H. Altemani5Mubarak A. Alzubaidi6Mohammed Almaghrabi7Adnan Al Harbi8Faisal Alsenani9Ahmed M. Sayed10Usama Ramadan Abdelmohsen11Department of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62511, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, MUST, Giza 12566, EgyptDepartment of Therapeutic Chemistry, Pharmaceutical and Drug Industries Research Institute, National Research Centre, El Bouhouth St., Dokki, Giza 12622, EgyptDepartment of Therapeutic Chemistry, Pharmaceutical and Drug Industries Research Institute, National Research Centre, El Bouhouth St., Dokki, Giza 12622, EgyptDepartment of Therapeutic Chemistry, Pharmaceutical and Drug Industries Research Institute, National Research Centre, El Bouhouth St., Dokki, Giza 12622, EgyptDepartment of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk 71491, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaPharmacognosy and Pharmaceutical Chemistry Department, Faculty of Pharmacy, Taibah University, Al Madinah Al Munawarah 42353, Saudi ArabiaClinical Pharmacy Department, College of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Pharmacognosy, College of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptThe protective and therapeutic anti-inflammatory and antioxidant potency of <i>Malapterurus electricus</i> (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints. Serum uric acid, TNF-α, IL-1β, NF-𝜅B, MDA, GSH, catalase, SOD, and glutathione reductase levels were all measured. According to the findings, FE significantly reduced uric acid levels and ankle swelling in both protective and therapeutic groups. Furthermore, it has anti-inflammatory effects by downregulating inflammatory cytokines, primarily through decreased oxidative stress and increased antioxidant status. All the aforementioned lesions were significantly improved in protected and treated rats with FE, according to histopathological findings. iNOS immunostaining revealed that protected and treated arthritic rats with FE had weak positive immune-reactive cells. Phytochemical analysis revealed that FE was high in fatty and amino acids. The most abundant compounds were vaccenic (24.52%), 9-octadecenoic (11.66%), palmitic (34.66%), stearic acids (14.63%), glycine (0.813 mg/100 mg), and alanine (1.645 mg/100 mg). Extensive molecular modelling and dynamics simulation experiments revealed that compound <b>4</b> has the potential to target and inhibit COX isoforms with a higher affinity for COX-2. As a result, we contend that FE could be a promising protective and therapeutic option for arthritis, aiding in the prevention and progression of this chronic inflammatory disease.https://www.mdpi.com/1660-3397/20/10/639<i>Malapterurus electricus</i>anti-inflammatoryCOX-2molecular dynamics simulation |
| spellingShingle | Abeer H. Elmaidomy Esraa M. Mohamed Hanan F. Aly Eman A. Younis Shams Gamal Eldin Shams Faisal H. Altemani Mubarak A. Alzubaidi Mohammed Almaghrabi Adnan Al Harbi Faisal Alsenani Ahmed M. Sayed Usama Ramadan Abdelmohsen Anti-Inflammatory and Antioxidant Properties of <i>Malapterurus electricus</i> Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects Marine Drugs <i>Malapterurus electricus</i> anti-inflammatory COX-2 molecular dynamics simulation |
| title | Anti-Inflammatory and Antioxidant Properties of <i>Malapterurus electricus</i> Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
| title_full | Anti-Inflammatory and Antioxidant Properties of <i>Malapterurus electricus</i> Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
| title_fullStr | Anti-Inflammatory and Antioxidant Properties of <i>Malapterurus electricus</i> Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
| title_full_unstemmed | Anti-Inflammatory and Antioxidant Properties of <i>Malapterurus electricus</i> Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
| title_short | Anti-Inflammatory and Antioxidant Properties of <i>Malapterurus electricus</i> Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
| title_sort | anti inflammatory and antioxidant properties of i malapterurus electricus i skin fish methanolic extract in arthritic rats therapeutic and protective effects |
| topic | <i>Malapterurus electricus</i> anti-inflammatory COX-2 molecular dynamics simulation |
| url | https://www.mdpi.com/1660-3397/20/10/639 |
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