The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA
Many cellular constituents travel along microtubules in association with multiple copies of motor proteins. How the activity of these motors is regulated during cargo sorting is poorly understood. In this study, we address this issue using a novel in vitro assay for the motility of localising Drosop...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2014-04-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/01596 |
| _version_ | 1811200139511988224 |
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| author | Harish Chandra Soundararajan Simon L Bullock |
| author_facet | Harish Chandra Soundararajan Simon L Bullock |
| author_sort | Harish Chandra Soundararajan |
| collection | DOAJ |
| description | Many cellular constituents travel along microtubules in association with multiple copies of motor proteins. How the activity of these motors is regulated during cargo sorting is poorly understood. In this study, we address this issue using a novel in vitro assay for the motility of localising Drosophila mRNAs bound to native dynein-dynactin complexes. High precision tracking reveals that individual RNPs within a population undergo either diffusive, or highly processive, minus end-directed movements along microtubules. RNA localisation signals stimulate the processive movements, with regulation of dynein-dynactin’s activity rather than its total copy number per RNP, responsible for this effect. Our data support a novel mechanism for multi-motor translocation based on the regulation of dynein processivity by discrete cargo-associated features. Studying the in vitro responses of RNPs to microtubule-associated proteins (MAPs) and microtubule ends provides insights into how an RNA population could navigate the cytoskeletal network and become anchored at its destination in cells. |
| first_indexed | 2024-04-12T01:58:44Z |
| format | Article |
| id | doaj.art-06991538df23481ab0cfb757cb1de745 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:58:44Z |
| publishDate | 2014-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-06991538df23481ab0cfb757cb1de7452022-12-22T03:52:43ZengeLife Sciences Publications LtdeLife2050-084X2014-04-01310.7554/eLife.01596The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNAHarish Chandra Soundararajan0Simon L Bullock1Division of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, United KingdomDivision of Cell Biology, MRC Laboratory of Molecular Biology, Cambridge, United KingdomMany cellular constituents travel along microtubules in association with multiple copies of motor proteins. How the activity of these motors is regulated during cargo sorting is poorly understood. In this study, we address this issue using a novel in vitro assay for the motility of localising Drosophila mRNAs bound to native dynein-dynactin complexes. High precision tracking reveals that individual RNPs within a population undergo either diffusive, or highly processive, minus end-directed movements along microtubules. RNA localisation signals stimulate the processive movements, with regulation of dynein-dynactin’s activity rather than its total copy number per RNP, responsible for this effect. Our data support a novel mechanism for multi-motor translocation based on the regulation of dynein processivity by discrete cargo-associated features. Studying the in vitro responses of RNPs to microtubule-associated proteins (MAPs) and microtubule ends provides insights into how an RNA population could navigate the cytoskeletal network and become anchored at its destination in cells.https://elifesciences.org/articles/01596molecular motormRNA localisationin vitro transportmicrotubules |
| spellingShingle | Harish Chandra Soundararajan Simon L Bullock The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA eLife molecular motor mRNA localisation in vitro transport microtubules |
| title | The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA |
| title_full | The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA |
| title_fullStr | The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA |
| title_full_unstemmed | The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA |
| title_short | The influence of dynein processivity control, MAPs, and microtubule ends on directional movement of a localising mRNA |
| title_sort | influence of dynein processivity control maps and microtubule ends on directional movement of a localising mrna |
| topic | molecular motor mRNA localisation in vitro transport microtubules |
| url | https://elifesciences.org/articles/01596 |
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