Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions
Abstract Background The genus Erianthus, which belongs to the “Saccharum complex”, includes C4 warm-season grasses. Erianthus species are widely distributed throughout Southeast Asia, East Asia and South Asia. Erianthus arundinaceus (Retz.) Jeswiet is highly adaptable to the environment, has a high...
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| Format: | Article |
| Language: | English |
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BMC
2022-01-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-021-03418-3 |
| _version_ | 1819261155403628544 |
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| author | Shin-ichi Tsuruta Suparat Srithawong Suchirat Sakuanrungsirikul Masumi Ebina Makoto Kobayashi Yoshifumi Terajima Amarawan Tippayawat Werapon Ponragdee |
| author_facet | Shin-ichi Tsuruta Suparat Srithawong Suchirat Sakuanrungsirikul Masumi Ebina Makoto Kobayashi Yoshifumi Terajima Amarawan Tippayawat Werapon Ponragdee |
| author_sort | Shin-ichi Tsuruta |
| collection | DOAJ |
| description | Abstract Background The genus Erianthus, which belongs to the “Saccharum complex”, includes C4 warm-season grasses. Erianthus species are widely distributed throughout Southeast Asia, East Asia and South Asia. Erianthus arundinaceus (Retz.) Jeswiet is highly adaptable to the environment, has a high percentage of dry matter, and is highly productive. Recently, this species has attracted attention as a novel bioenergy crop and as a breeding material for sugarcane improvement. Such interest in E. arundinaceus has accelerated the collection and conservation of its genetic resources, mainly in Asian countries, and also evaluation of morphological, agricultural, and cytogenetic features in germplasm collections. In Thailand, genetic resources of E. arundinaceus have been collected over the past 20 years and their phenotypic traits have been evaluated. However, the genetic differences and relatedness of the germplasms are not fully understood. Results A set of 41 primer pairs for nuclear simple sequence repeats (SSRs) developed from E. arundinaceus were used to assess the genetic diversity of 121 Erianthus germplasms collected in Thailand; of these primer pairs, 28 detected a total of 316 alleles. A Bayesian clustering approach with these alleles classified the accessions into four main groups, generally corresponding to the previous classification based on phenotypic analysis. The results of principal coordinate analysis and phylogenetic analysis of the 121 accessions on the basis of the SSR markers showed the same trend as Bayesian clustering, whereas sequence variations of three non-coding regions of chloroplast DNA revealed eight haplotypes among the accessions. The analysis of genetic structure and phylogenetic relationships, however, found some accessions whose classification contradicted the results of previous phenotypic classification. Conclusions The molecular approach used in this study characterized the genetic diversity and relatedness of Erianthus germplasms collected across Thailand. This knowledge would allow efficient maintenance and conservation of the genetic resources of this grass and would help to use Erianthus species as breeding materials for development of novel bioenergy crops and sugarcane improvement. |
| first_indexed | 2024-12-23T19:37:18Z |
| format | Article |
| id | doaj.art-069b3c58538d40f0b7050f4785ee5dfe |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-23T19:37:18Z |
| publishDate | 2022-01-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-069b3c58538d40f0b7050f4785ee5dfe2022-12-21T17:33:45ZengBMCBMC Plant Biology1471-22292022-01-0122111610.1186/s12870-021-03418-3Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessionsShin-ichi Tsuruta0Suparat Srithawong1Suchirat Sakuanrungsirikul2Masumi Ebina3Makoto Kobayashi4Yoshifumi Terajima5Amarawan Tippayawat6Werapon Ponragdee7Tropical Agriculture Research Front, Japan International Research Center for Agricultural Sciences (JIRCAS)Department of Agriculture, Khon Kaen Field Crops Research Center (KKFCRC)Department of Agriculture, Khon Kaen Field Crops Research Center (KKFCRC)Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO)Tropical Agriculture Research Front, Japan International Research Center for Agricultural Sciences (JIRCAS)Department of Agriculture, Khon Kaen Field Crops Research Center (KKFCRC)Department of Agriculture, Khon Kaen Field Crops Research Center (KKFCRC)Abstract Background The genus Erianthus, which belongs to the “Saccharum complex”, includes C4 warm-season grasses. Erianthus species are widely distributed throughout Southeast Asia, East Asia and South Asia. Erianthus arundinaceus (Retz.) Jeswiet is highly adaptable to the environment, has a high percentage of dry matter, and is highly productive. Recently, this species has attracted attention as a novel bioenergy crop and as a breeding material for sugarcane improvement. Such interest in E. arundinaceus has accelerated the collection and conservation of its genetic resources, mainly in Asian countries, and also evaluation of morphological, agricultural, and cytogenetic features in germplasm collections. In Thailand, genetic resources of E. arundinaceus have been collected over the past 20 years and their phenotypic traits have been evaluated. However, the genetic differences and relatedness of the germplasms are not fully understood. Results A set of 41 primer pairs for nuclear simple sequence repeats (SSRs) developed from E. arundinaceus were used to assess the genetic diversity of 121 Erianthus germplasms collected in Thailand; of these primer pairs, 28 detected a total of 316 alleles. A Bayesian clustering approach with these alleles classified the accessions into four main groups, generally corresponding to the previous classification based on phenotypic analysis. The results of principal coordinate analysis and phylogenetic analysis of the 121 accessions on the basis of the SSR markers showed the same trend as Bayesian clustering, whereas sequence variations of three non-coding regions of chloroplast DNA revealed eight haplotypes among the accessions. The analysis of genetic structure and phylogenetic relationships, however, found some accessions whose classification contradicted the results of previous phenotypic classification. Conclusions The molecular approach used in this study characterized the genetic diversity and relatedness of Erianthus germplasms collected across Thailand. This knowledge would allow efficient maintenance and conservation of the genetic resources of this grass and would help to use Erianthus species as breeding materials for development of novel bioenergy crops and sugarcane improvement.https://doi.org/10.1186/s12870-021-03418-3Erianthus arundinaceusSaccharumBioenergyGermplasmSSRGenetic diversity |
| spellingShingle | Shin-ichi Tsuruta Suparat Srithawong Suchirat Sakuanrungsirikul Masumi Ebina Makoto Kobayashi Yoshifumi Terajima Amarawan Tippayawat Werapon Ponragdee Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions BMC Plant Biology Erianthus arundinaceus Saccharum Bioenergy Germplasm SSR Genetic diversity |
| title | Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions |
| title_full | Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions |
| title_fullStr | Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions |
| title_full_unstemmed | Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions |
| title_short | Erianthus germplasm collection in Thailand: genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions |
| title_sort | erianthus germplasm collection in thailand genetic structure and phylogenetic aspects of tetraploid and hexaploid accessions |
| topic | Erianthus arundinaceus Saccharum Bioenergy Germplasm SSR Genetic diversity |
| url | https://doi.org/10.1186/s12870-021-03418-3 |
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