Green Extraction-Assisted Pseudo-Targeted Profile of Alkaloids in Lotus Seed Epicarp Based on UPLC-QTOF MS with IDA
Lotus seed epicarp, a byproduct of lotus, is commonly discarded directly or burned in the cropland, resulting in waste of resources and environmental pollution. In this work, a green ultrasonic-assisted extraction method with ethyl lactate as the extraction solvent was established to extract alkaloi...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/7/1056 |
| _version_ | 1827623466961993728 |
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| author | Xiaoji Cao Xupin Lin Congcong Wu Minghua Zhang Mingwei Wang |
| author_facet | Xiaoji Cao Xupin Lin Congcong Wu Minghua Zhang Mingwei Wang |
| author_sort | Xiaoji Cao |
| collection | DOAJ |
| description | Lotus seed epicarp, a byproduct of lotus, is commonly discarded directly or burned in the cropland, resulting in waste of resources and environmental pollution. In this work, a green ultrasonic-assisted extraction method with ethyl lactate as the extraction solvent was established to extract alkaloids from lotus seed epicarp. The extraction conditions were optimized by response surface methodology. Under the optimal extraction conditions, the extraction of alkaloids from 1 g lotus seed epicarp was accomplished with only 10 mL of extraction solvent within 15 min. Combined with ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry with information-dependent acquisition mode, a total of 42 alkaloids were annotated in the lotus seed epicarp extracts. Among them, 39 alkaloids were reported in lotus seed epicarp for the first time. According to quantitative analysis, the distributions and trends of alkaloids in the lotus seed epicarp were found to be similar to those of lotus leaves. The five growth stages of lotus seed epicarp could be successfully distinguished based on the ten representative alkaloids. This study demonstrates that ultrasonic-assisted extraction with ethyl lactate as extractant solvent was efficient in the extraction of alkaloids from lotus seed epicarp, which is a potential renewable resource of bioactive ingredients. |
| first_indexed | 2024-03-09T11:49:05Z |
| format | Article |
| id | doaj.art-069f366388dd404dbada99970c36aca3 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T11:49:05Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-069f366388dd404dbada99970c36aca32023-11-30T23:16:26ZengMDPI AGFoods2304-81582022-04-01117105610.3390/foods11071056Green Extraction-Assisted Pseudo-Targeted Profile of Alkaloids in Lotus Seed Epicarp Based on UPLC-QTOF MS with IDAXiaoji Cao0Xupin Lin1Congcong Wu2Minghua Zhang3Mingwei Wang4Research Center of Analysis and Measurement, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Chemical Engineering, Zhejiang University of Technology, Hangzhou 310014, ChinaLotus seed epicarp, a byproduct of lotus, is commonly discarded directly or burned in the cropland, resulting in waste of resources and environmental pollution. In this work, a green ultrasonic-assisted extraction method with ethyl lactate as the extraction solvent was established to extract alkaloids from lotus seed epicarp. The extraction conditions were optimized by response surface methodology. Under the optimal extraction conditions, the extraction of alkaloids from 1 g lotus seed epicarp was accomplished with only 10 mL of extraction solvent within 15 min. Combined with ultrahigh-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry with information-dependent acquisition mode, a total of 42 alkaloids were annotated in the lotus seed epicarp extracts. Among them, 39 alkaloids were reported in lotus seed epicarp for the first time. According to quantitative analysis, the distributions and trends of alkaloids in the lotus seed epicarp were found to be similar to those of lotus leaves. The five growth stages of lotus seed epicarp could be successfully distinguished based on the ten representative alkaloids. This study demonstrates that ultrasonic-assisted extraction with ethyl lactate as extractant solvent was efficient in the extraction of alkaloids from lotus seed epicarp, which is a potential renewable resource of bioactive ingredients.https://www.mdpi.com/2304-8158/11/7/1056lotus seed epicarpalkaloidsbio-based solventspseudo-targeted profileUPLC-QTOF MS |
| spellingShingle | Xiaoji Cao Xupin Lin Congcong Wu Minghua Zhang Mingwei Wang Green Extraction-Assisted Pseudo-Targeted Profile of Alkaloids in Lotus Seed Epicarp Based on UPLC-QTOF MS with IDA Foods lotus seed epicarp alkaloids bio-based solvents pseudo-targeted profile UPLC-QTOF MS |
| title | Green Extraction-Assisted Pseudo-Targeted Profile of Alkaloids in Lotus Seed Epicarp Based on UPLC-QTOF MS with IDA |
| title_full | Green Extraction-Assisted Pseudo-Targeted Profile of Alkaloids in Lotus Seed Epicarp Based on UPLC-QTOF MS with IDA |
| title_fullStr | Green Extraction-Assisted Pseudo-Targeted Profile of Alkaloids in Lotus Seed Epicarp Based on UPLC-QTOF MS with IDA |
| title_full_unstemmed | Green Extraction-Assisted Pseudo-Targeted Profile of Alkaloids in Lotus Seed Epicarp Based on UPLC-QTOF MS with IDA |
| title_short | Green Extraction-Assisted Pseudo-Targeted Profile of Alkaloids in Lotus Seed Epicarp Based on UPLC-QTOF MS with IDA |
| title_sort | green extraction assisted pseudo targeted profile of alkaloids in lotus seed epicarp based on uplc qtof ms with ida |
| topic | lotus seed epicarp alkaloids bio-based solvents pseudo-targeted profile UPLC-QTOF MS |
| url | https://www.mdpi.com/2304-8158/11/7/1056 |
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