Bone Morphogenetic Protein 7 Effect on Human Glioblastoma Cell Transmigration and Migration
Glioblastoma, World Health Organization—grade IV, is the most malignant glioma type and it is still an incurable tumor due to the high level of heterogeneity and uncontrolled metastatic nature. In addition to the tumorigenicity-suppressing activity, bone morphogenetic protein 7 (BMP7) has recently b...
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MDPI AG
2021-07-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/11/7/708 |
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| author | Ting-Chung Wang Sheng-Jie Luo Shun-Fu Chang |
| author_facet | Ting-Chung Wang Sheng-Jie Luo Shun-Fu Chang |
| author_sort | Ting-Chung Wang |
| collection | DOAJ |
| description | Glioblastoma, World Health Organization—grade IV, is the most malignant glioma type and it is still an incurable tumor due to the high level of heterogeneity and uncontrolled metastatic nature. In addition to the tumorigenicity-suppressing activity, bone morphogenetic protein 7 (BMP7) has recently been found for its invasion-promoting role in glioblastoma. However, the detailed and precise mechanism in this issue should have more elucidation. Thus, in this study, we determined the BMP7 effect on glioblastoma transmigration and migration regulations and the underlying mechanisms. Human LN18/LN229 glioblastoma cells were used in this study. Our results showed a higher BMP7/pSmad5 level in human malignant glioma tissues compared to healthy brain tissues. In addition, it was demonstrated that endogenous and exogenous BMP7 stimulation could increase the transmigration and migration capabilities of human LN18/LN229 glioblastoma cells. Moreover, this event is regulated by Smad5 and p75 neurotrophin receptor (p75NTR) signaling. Furthermore, unexpected data are that the Smad1 gene knockdown could lead to the cell death of human LN18 glioblastoma cells. Overall, the present study finds that the invasion-promoting activity of BMP7 might be an autocrine stimulation of glioblastoma and this effect could be regulated by Smad5-p75NTR signaling. |
| first_indexed | 2024-03-10T09:33:59Z |
| format | Article |
| id | doaj.art-06a1ba62ae4b4f8b89601de32e44bd82 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T09:33:59Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-06a1ba62ae4b4f8b89601de32e44bd822023-11-22T04:13:31ZengMDPI AGLife2075-17292021-07-0111770810.3390/life11070708Bone Morphogenetic Protein 7 Effect on Human Glioblastoma Cell Transmigration and MigrationTing-Chung Wang0Sheng-Jie Luo1Shun-Fu Chang2Department of Neurosurgery, Chiayi Chang Gung Memorial Hospital, Chiayi 613, TaiwanDepartment of Neurosurgery, Chiayi Chang Gung Memorial Hospital, Chiayi 613, TaiwanDepartment of Medical Research and Development, Chiayi Chang Gung Memorial Hospital, Chiayi 613, TaiwanGlioblastoma, World Health Organization—grade IV, is the most malignant glioma type and it is still an incurable tumor due to the high level of heterogeneity and uncontrolled metastatic nature. In addition to the tumorigenicity-suppressing activity, bone morphogenetic protein 7 (BMP7) has recently been found for its invasion-promoting role in glioblastoma. However, the detailed and precise mechanism in this issue should have more elucidation. Thus, in this study, we determined the BMP7 effect on glioblastoma transmigration and migration regulations and the underlying mechanisms. Human LN18/LN229 glioblastoma cells were used in this study. Our results showed a higher BMP7/pSmad5 level in human malignant glioma tissues compared to healthy brain tissues. In addition, it was demonstrated that endogenous and exogenous BMP7 stimulation could increase the transmigration and migration capabilities of human LN18/LN229 glioblastoma cells. Moreover, this event is regulated by Smad5 and p75 neurotrophin receptor (p75NTR) signaling. Furthermore, unexpected data are that the Smad1 gene knockdown could lead to the cell death of human LN18 glioblastoma cells. Overall, the present study finds that the invasion-promoting activity of BMP7 might be an autocrine stimulation of glioblastoma and this effect could be regulated by Smad5-p75NTR signaling.https://www.mdpi.com/2075-1729/11/7/708bone morphogenetic protein 7glioblastomap75 neurotrophin receptorSmad5 |
| spellingShingle | Ting-Chung Wang Sheng-Jie Luo Shun-Fu Chang Bone Morphogenetic Protein 7 Effect on Human Glioblastoma Cell Transmigration and Migration Life bone morphogenetic protein 7 glioblastoma p75 neurotrophin receptor Smad5 |
| title | Bone Morphogenetic Protein 7 Effect on Human Glioblastoma Cell Transmigration and Migration |
| title_full | Bone Morphogenetic Protein 7 Effect on Human Glioblastoma Cell Transmigration and Migration |
| title_fullStr | Bone Morphogenetic Protein 7 Effect on Human Glioblastoma Cell Transmigration and Migration |
| title_full_unstemmed | Bone Morphogenetic Protein 7 Effect on Human Glioblastoma Cell Transmigration and Migration |
| title_short | Bone Morphogenetic Protein 7 Effect on Human Glioblastoma Cell Transmigration and Migration |
| title_sort | bone morphogenetic protein 7 effect on human glioblastoma cell transmigration and migration |
| topic | bone morphogenetic protein 7 glioblastoma p75 neurotrophin receptor Smad5 |
| url | https://www.mdpi.com/2075-1729/11/7/708 |
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